病毒白细胞介素-10转基因在造血干细胞中的表达

目的　观察逆转录病毒转病毒白细胞介素 10 (vIL 10 )基因在体内的表达。方法　用MSCVneo vIL 10重组体在体外转导CBA (H 2 K)小鼠的造血干细胞 (HSCs) ,给经致死照射(90 0rads)的 2 0只同基因CBA(H 2 K)小鼠注入经MSCVneo vIL 10转染的HSCs ,2× 10 6HSCs/只。酶联免疫吸附测定 (ELISA)、逆转录 聚合酶链反应 (RT PCR )、Westernblot分析vIL 10的表达。结果　移植MSCVneo vIL 10转染HSCs的 2 0只小鼠 ,移植后 8周用ELISA检测 ,其中 15只小鼠血清的vIL 10浓度为 :2 70～ 13 40ng/L ,5只小鼠血清的vIL 10为阴性。 12周后有 2只小鼠vIL 10测不出 ,13只小鼠长时间表达vIL 10达 6个月。对照组小鼠血清vIL 10均为阴性。RT PCR和Westernblot证实小鼠的器官均有vIL 10的mRNA和蛋白的表达。结论　逆转录病毒能有效地将vIL 10基因导入造血干细胞并在体内长时间表达。     

正常成人脑外段视神经的MRI研究

目的 评价正常成人视神经-眼平面(NOP)视交叉以外段颅神经的MRI表现,建立正常标准。方法 随机选择正常成人志愿者50例,采用GB1.5T MR/i echo spood plus超导型磁共振成像仪及相控阵头线圈,成像序列包括自旋回波T1加权成像(SET1WI)和自旋回波T2加权成像(SET2WI);扫描方位以NOP平面为基准扫描。分别测量两组视神经眼球后神经膨大段直径、眶内段视神经直径、长度,管内段视神经直径及该段视神经起始至同侧视交叉的长度,后者与眶内段视神经长度之和即代表颅外段视神经的长度。结果 NOP平面上,正常人眼球后神经膨大段直径为4.2±0.56mm,眶内段直径为3.1±0.45mm、长度为24.6±2.88mm,管内段视神经直径为3.2±0.49mm,至同侧视交叉的长度为18.7±2.40mm,颅外段视神经的长度为43.3±3.83mm。统计分析,正常成人组内性别和年龄、双侧视神经之间无显著差异。 结论 NOP平面评价视神经有很大优势,MRI可以很好显示视神经的形态,是研究其解剖和疾病的有效检查手段。     

Cyclin E, p27 and mutant p53 do not predict the prognosis in AJCC stage II colorectal carcinomas.

BACKGROUND/AIMS: Carcinogenesis is characterized by the abnormal regulation of cell cycle. The abnormal expression of the regulators of cell cycle may be related to the prognosis. Since the clinical significance of the expression of the three proteins in colorectal carcinomas is still controversial, we evaluated the prognostic value of the expression of cyclin E, p27 and mutant p53 in stage II colorectal cancer. METHODS: The expression levels of cyclin E, p27 and mutant p53 proteins in 41 patients with stage II colorectal carcinomas were analyzed by immunohistochemistry. RESULTS: In the univariate analysis, the level of CEA at diagnosis was associated with disease relapse. In the multivariate analysis, the clinicopathological variables such as age, gender, site of primary tumor, tumor size, state of tumor differentiation and preoperative plasma CEA level were not associated with disease relapse. When Kaplan-Meier survival curves were constructed to determine the prognosis, cyclin E, p27 and mutant p53 expressions did not predict poor prognosis. CONCLUSIONS: Our results suggested that the expression of cyclin E, p27 and mutant p53 proteins did not predict the clinical outcome in the stage II colorectal carcinomas.     

肝移植患者术后早期精神症状的观察

目的探讨肝脏移植术后早期精神系统并发症发生的原因和防治经验。方法回顾性分析 12 5例原位肝脏移植患者的临床资料 ,以术后 2周作为观察时点 ,分析肝脏移植术后早期精神系统并发症发生的原因 ,总结防治经验。结果有症状组和无症状组在性别、年龄、肝功能以及血环孢素A浓度方面无明显差异 ;但有症状组的无肝期时间 (93 74± 2 8 98)min和手术时间 (4 14 6 5±6 1 92 )min却长于无症状组 (P <0 0 5 ) ;另外 ,术前有无肝性脑病、术后感染以及静脉使用免疫抑制剂和术后精神症状的发生明显相关。结论肝脏移植术后早期精神系统并发症发生的原因是多方面的 ,通过积极的对症支持治疗 ,预后良好。     

某医院传染性非典型肺炎疫情分析

目的：分析某医院SARS疫情的发生、发展及控制过程，为疫情控制提供一定的参考依据。方法：依照卫生部推荐的统一个案调查表和临床诊断标准，描述疫情的三间分布以及发放防护用品的数量与病例数的关系。结果：某医院疫情暴发曲线由3个暴发高峰组成，前两个峰较大，后一个峰较小，呈散发。提示病例传染共形成“三代”流行。结论：采用严密的综合防护措施，能够彻底控制SARS疫情在医院的暴发。     

腹股沟疝不同手术方式修补的比较

目的探讨腹股沟疝不同修补手术方法的优缺点.方法75例腹股沟疝行腹腔镜疝修补术,96例腹股沟疝行Bassini疝修补术,记录手术时间、术后下床活动时间、住院时间及各种并发症并进行比较.结果腹腔镜组手术全部成功完成,8例病人同时行阑尾切除术、4例病人同时行胆囊切除术.腹腔镜组手术时间、术后下床活动时间、住院时间较Bassini组少(P＜0.05).Bassini组术后并发症较腹腔镜组多,(χ 2=14.3339,P＜0.01).腹腔镜组1例(1.33%)复发,Bassini组4例(4.25%)复发,差异无显著性(P＞0.05).结论腹腔镜疝修补术是一种安全而有效的无张力疝修补手术,较Bassini组手术后恢复时间短,术后并发症少.     

剖宫产术中大出血填塞纱条止血53例临床分析

目的观察宫腔填塞纱条对剖宫产术中大出血的止血效果。方法剖宫产术中大出血患者53例,常规处理方法无效后,采用宫腔填塞纱条止血,术中宫体注射缩宫素。以同期剖宫产50例为对照组,采用宫体注射缩宫素20U。结果53例经宫腔填塞纱条后,52例能迅速控制出血,有效率98.1%。与对照组比较,两组术后24h出血量、术后5d、42d子宫径线和无显著差异(P>0.05)。结论宫腔填塞纱条治疗剖宫产术中大出血有积极作用。     

体外冲击波碎石术后石街的处理(附41例报告)

目的总结体外冲击波碎石(ESWL)术后石街的处理经验。方法回顾性分析2001年8月至2006年3月我院41例上尿路结石行ESWL术后形成石街患者的临床资料。结果9例石街采用逆行引流体位后自行排净,25例再次行ESWL术后石街排净,5例改行输尿管镜碎石术,2例行开放手术。结论采用患侧卧位的逆行引流体位,再次行ESWL术和输尿管镜碎石术等方法处理,能有效提高石街的排净率。     

阿尔茨海默病中肿瘤坏死因子-α基因多态性及表达

目的探讨肿瘤坏死因子-α(TNF-α)基因多态性与广东地区汉族人群散发性阿尔茨海默病(SAD)发病易感性的关系。方法采用聚合酶链反应-序列特异性引物(PCR-SSP)方法,检测44例SAD病人(AD组)及45例正常老年人(对照组)TNF-α基因的TNF-α1及TNF-α2等位基因频率。按比值比(OR)作疾病关联分析。用放免法检测两组的血清TNF-α含量。结果AD组的TNF-α2基因频率为0．182,明显高于对照组的0．078(OR=2．635,P=0．039)；AD组血清TNF-α含量为(567．85±102．43) ng/L,明显高于对照组的(389．73±56．68)ng/L(P＜0．05)。结论广东地区汉族人群中,TNF-α基因多态性与SAD有关联．TNF-α2等位基因与SAD的易感性有关。     

Clinical usefulness of the frontal assessment battery (FAB) for elderly in memory clinic]

To investigate the usefulness of the frontal assessment battery (FAB) as a brief assessment for diagnosis in patients with dementia, we examined cognitive function using the FAB, Mini-mental state examination (MMSE), Kana-hiroi test, and Raven's colored progressive matrices (RCPM). Subjects were 82 senile patients having amnesia as a complaint including 28 Alzheimer's disease (AD), 14 frontotemporal dementia (FTD), 17 vascular dementia (VD), 13 mild cognitive impairment (MCI), and 10 healthy persons. We discussed the difference of scores in different types of dementia. In the FAB, the scores of healthy persons and MCI were much higher compared with the scores of all types of dementia. There were high correlation between FAB and MMSE, Kana-hiroi test, and RCPM. Although some subjects showed normal score in MMSE and/or RCPM, they showed low score in FAB. Based on this, FAB is efficient for the differential diagnosis of dementia as brief screening tool.