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101.
Protein kinase C gamma interneurons in the rat medullary dorsal horn: Distribution and synaptic inputs to these neurons,and subcellular localization of the enzyme 下载免费PDF全文
Cédric Peirs Sudarshan Patil Rabia Bouali‐Benazzouz Alain Artola Marc Landry Radhouane Dallel 《The Journal of comparative neurology》2014,522(2):393-413
The γ isoform of protein kinase C (PKCγ), which is concentrated in interneurons in the inner part of lamina II (IIi) of the dorsal horn, has been implicated in the expression of tactile allodynia. Lamina IIi PKCγ interneurons were shown to be activated by tactile inputs and to participate in local circuits through which these inputs can reach lamina I, nociceptive output neurons. That such local circuits are gated by glycinergic inhibition and that A‐ and C‐fibers low threshold mechanoreceptors (LTMRs) terminate in lamina IIi raise the general issue of synaptic inputs to lamina IIi PKCγ interneurons. Combining light and electron microscopic immunochemistry in the rat spinal trigeminal nucleus, we show that PKCγ‐immunoreactivity is mostly restricted to interneurons in lamina IIi of the medullary dorsal horn, where they constitute 1/3 of total neurons. The majority of synapses on PKCγ‐immunoreactive interneurons are asymmetric (likely excitatory). PKCγ‐immunoreactive interneurons appear to receive exclusively myelinated primary afferents in type II synaptic glomeruli. Neither large dense core vesicle terminals nor type I synaptic glomeruli, assumed to be the endings of unmyelinated nociceptive terminals, were found on these interneurons. Moreover, there is no vesicular glutamate transporter 3‐immunoreactive bouton, specific to C‐LTMRs, on PKCγ‐immunoreactive interneurons. PKCγ‐immunoreactive interneurons contain GABAAergic and glycinergic receptors. At the subcellular level, PKCγ‐immunoreactivity is mostly concentrated on plasma membranes, close to, but not within, postsynaptic densities. That only myelinated primary afferents were found to contact PKCγ‐immunoreactive interneurons suggests that myelinated, but not unmyelinated, LTMRs play a critical role in the expression of mechanical allodynia. J. Comp. Neurol. 522:393–413, 2014. © 2013 Wiley Periodicals, Inc. 相似文献
102.
高血压病胰岛素抵抗与红细胞内钠,钾,镁离子变化的研究 总被引:2,自引:1,他引:1
测定30例高血压病(EH)患者和16例正常血压(NT)对照组禁食及75g葡萄糖负荷后30,60,120min血浆葡萄糖和胰岛素浓度,红细胞内钠,钾,镁离子浓度。结果表明EH患者禁食30,60min血浆葡萄糖和禁食30,60,120min血浆胰岛素浓度和Na^+显著高于NT对照组,而K^+,Mg^2+显著低于NT对照组。简单相关,分析显示EH组空腹血浆胰岛素与体重,体重指数,臀围显著正相关,胰岛素曲 相似文献
103.
104.
N. Charfi N. Smaoui M. Turki M. Maâlej Bouali S. Omri J. Ben Thabet N. Zouari L. Zouari M. Maâlej 《Revue d'épidémiologie et de santé publique》2019,67(1):13-20
Background
Although the increase of media interest for psychoactive substances use, especially alcohol, among young people, since the revolution, Tunisia has few epidemiological studies on this subject, which does not allow having a clear idea of the importance of the problem and its different determinants.Aims
To assess the prevalence of alcohol consumption among adolescents in the region of Sfax (Tunisia) and to determine its relations to the two personality dimensions: sensation seeking and impulsivity.Methods
This was a cross-sectional study involving 317 middle and high school students in Sfax (Tunisia). We used the Alcohol Use Disorders Identification Test (AUDIT) to assess risky alcohol-consumption, the Barratt Impulsiveness Scale (BIS-11) to assess the degree of impulsivity and the Sensation Seeking Scale (SSS-V) to assess the level of sensation seeking.Results
The average age of students was 15.79 ± 1.5 years with a sex-ratio of 1.07. The prevalence of experimenters (who had drunk alcohol at least once in their lifetime) was 19.6% while that of current consumers (who had drunk alcohol more than one time during the past 12 months before the survey) was 8.8%. Among those who reported alcohol use during the last year, 42.8% were alcohol-dependent, according to the AUDIT. The analysis of personality dimensions showed a significant association between current alcohol consumption and sensation seeking (P < 0.001) in particular on the dimensions of disinhibition (DIS), experience seeking (ES) and boredom susceptibility (BS) (P respectively: <0.001; 0.002 and 0.001). Total impulsivity and motor and attention impulsivity were associated with current alcohol consumption (respective P: 0.001, 0.005 and 0.015).Conclusion
Our study of schooled Tunisian adolescents shows that sensation seeking and impulsivity are associated with the development of alcohol use. Such a behavior is worrisome because it appears to be quite frequent and often problematic. 相似文献105.
Ben Thabet Jihene Charfi Nada Dalhoum Wafa Yaich Sourour Maâlej Bouali Manel Omri Sana Zouari Nasreddine Zouari Lobna Damak Jamel Maâlej Mohamed 《The Psychiatric quarterly》2019,90(1):29-40
Psychiatric Quarterly - Schizophrenia is often accompanied by somatic comorbidities, which make the management challenge of such patients more difficult. In this study, we proposed to identify the... 相似文献
106.
Sennana Sendi H Elghezal H Temmi H Gribaa M Laatiri A Ben Abid H Ben Abdeladhime A Elloumi M Hafsia A Saad A 《La Tunisie médicale》2002,80(9):531-535
A group of 139 patients with de novo acute myeloid leukemia were investigated to determine the prognostic significance of karyotype on early death, complete remission, continuous complete remission and survival. There were 27 children and 112 adults. Mean age was 32 years. t(15;17) was found associated with a high rate of early death and a diploid karyotype with long continuous complete remission. The presence of a structural change was predictive of shorter survivals. The study of the prognostic impact of recurrent anomalies reveals a good prognostic impact for normal karyotype (1 year survival probability: 40%), followed by t(8;21) (1 year survival probability: 24%), and by t(15;17) (1 year survival probability: 9%). 相似文献
107.
Ndoye A Bouali S Dolivet G Leroux A Erbacher P Behr JP Berg K Guillemin F Merlin JL 《International journal of oncology》2004,25(6):1575-1581
p53 is frequently mutated in head-and-neck squamous cell carcinoma. Wild-type p53 gene transfer induces apoptosis in vitro and tumor regression in vivo and clinical investigations of p53 gene therapy have been reported, mostly using viral vectors. Non-viral vectors are increasingly being used as an alternative to viral vectors and photochemical internalisation (PCI) of non-viral vectors has been reported to yield high gene transfer efficiency. The p53-mutated status of FaDu human pharynx carcinoma cell line was first assessed by DNA sequencing and the cells were transfected using tetraglucosylated polyethylenimine (PEI-Glu4) in conjunction with photochemical internalisation (PCI). The green fluorescent protein (GFP) was used as a reporter for determination of the transgene expression kinetics with or without PCI. p53 gene transfer was performed in these optimised conditions, and subsequent induction of apoptosis was investigated by flow cytometric determination of the phosphatidylserine externalization. Long-term cell death was assessed using colony forming assays. DNA sequencing in FaDu cells showed a G/T point mutation at codon 248 in exon 7 of p53 gene, resulting in an arginine-to-leucine substitution. As a consequence, P53 was shown to be expressed in >90% of untreated cells using immunocytochemistry. Using PEI-Glu4 as vector, PCI was found to significantly enhance GFP gene transfer whatever the formulation solution. Transfection efficiency was significantly increased with PCI. GFP expression kinetics (24-144 h) demonstrates that PCI induces sustained transgene expression with >10% of cells remaining transfected after 144 h. In such conditions, p53 gene transfer using PEI-Glu4 and PCI, resulted in spontaneous induction of apoptosis. As a consequence, long-term cell death was significantly enhanced after wt-p53 gene transfer when PCI was used, reaching up to 50% cell death. Wild-type p53 gene transfer using PEI-Glu4/DNA complexes and PCI, yields sustained transgene expression and induces cell death in p53-mutated FaDu cells. 相似文献
108.
There is little information on the epidemiology characterisation of HCV isolates in Tunisia. Previous report showed predominance of genotype 1b. In this study, 32 HCV isolates from genotypes 1a (n = 10), 1b (n = 14), 2 (n = 4), 3a (n = 3) and 4 (n = 1) were genotyped by sequencing and phylogenetic analysis on the non-structural 5b (NS5b) region. The isolates originated from 14 patients with chronic hepatitis, 10 haemophiliacs and eight healthy blood donors. NS5b sequence grouping was concordant with previous 5' untranslated region (5'UTR) genotyping results in 91% of cases. Most of the Tunisian isolates were closely related to the European ones, except for genotype 4 which seems to be related mostly to isolates from Egypt. Isolates from genotype 1a obtained from haemophiliacs showed distinct clustering and nucleic divergence from those obtained from non-haemophiliac patients, this underlines the particular mode of contamination of this group of patients. 相似文献
109.
Ben Amara F Idrissi A Khemiri H Ben Amar S Guermazi M Bouzid F Rekik S 《La Tunisie médicale》2004,82(2):223-228
We report 7 cases of leiomyosarcoma of the uterus observed in the obstetrical and gynaecological department of Sfax (Tunisia). It's a rare tumour with difficult diagnosis and poor prognosis. It requires mainly a surgical treatment and radiotherapy. 相似文献
110.