Influence of leptin on luteinizing hormone and follicle stimulating hormone secreted from cultured rat anterior pituitary cells

BACKGROUND: Leptin may regulate reproductive function via release of hypothalamic neuropeptide Y. However, it is unknown whether this regulatory effect is limited to the hypothalamus. OBJECTIVE: To detect the effect of different dosages of leptin on luteinizing hormone (LH) and follicle stimulating hormone (FSH) secretion from in vitro cultured rat anterior pituitary cells. DESIGN: Contrast study based on cells. SETTING: This study was performed in the Basic Institute of Chengde Medical College, Chengde City, Hebei Province, China from March to June 2007. MATERIALS: Eighteen female Wistar rats of three months of age, weighing 200-220 g, and of clean grade were used. Leptin was provided by Peprotech Company, DMEM culture medium by Invitrogen Company, and the radioimmunological kit by Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. METHODS: Three glandular organs were regarded as one group for culture of anterior pituitary cells. In the control group, saline was added to the culture medium instead of leptin. In the leptin group, leptin was prepared into different concentrations of 1×10-12, 1×10-11, 1×10-9, 1×10-7, and 1×10-6 mol/L for stimulation of cultured cells. The culture supernatant was obtained at three hours after additional of saline/leptin. MAIN OUTCOME MEASURES: Contents of LH and FSH were detected by radioimmunology. RESULTS: Following leptin stimulation, LH release increased with increasing concentrations of leptin up to 1×10-9 mol/L, where LH release peaked. LH release then progressively decreased with increasing leptin concentrations (P<0.01). LH release in the leptin (1×10-12, 1×10-11, 1×10-7, and 1×10-6 mol/L) groups was significantly higher than that in the control group (P<0.01). FSH content in the leptin (1×10-11, 1×10-9, and 1×10-7 mol/L) groups was significantly higher than that in the control group (P<0.01). CONCLUSION: Leptin can directly affect pituitary tissue to promote the secretion of LH and FSH in a dose-dependent manner.     

MRP、P-gp在非小细胞肺癌中的表达及临床意义

目的　观察多药耐药相关蛋白 (MRP)、P-糖蛋白 (P- gp)在肺癌组织中的表达情况及与临床病理的关系 ,以及 MRP与 P- gp之间的相关性 ,探讨 MRP、P- gp与肺癌患者预后的关系。方法　应用 S- P免疫组化法检测非小细胞肺癌 MRP、P- gp的表达情况。结果　 4 2例非小细胞肺癌中 MRP阳性率为 4 5 .2 % ,腺癌 MRP的表达明显高于鳞癌 (P<0 .0 5 )。 P- gp阳性表达率为 4 0 .4 % ,P- gp的表达与组织类型、细胞分化程度、TNM临床分期无关 (P>0 .0 5 )。 MRP、P- gp阴性患者术后 1、3年无病生存期 (DFS)明显高于 MRP、P- gp阳性患者 (P<0 .0 1)。肺癌组织中 P- gp与 MRP的表达具有明显相关性 (P<0 .0 5 )。结论　 MRP、P- gp可作为临床判断化疗疗效的参考指标及预测肺癌患者预后的指标     

益肾降浊汤对脑缺血再灌注大鼠海马EAA含量的影响

目的 :观察益肾降浊汤对脑缺血再灌注大鼠海马组织EAA含量的影响。方法 :用氨基酸自动分析仪测定游离氨基酸浓度。结果 :第 1天模型大鼠海马Glu、Asp含量分别是 ( 8.2 6± 2 .13 ) μmol/g、( 2 .10± 0 .93 ) μmol/g ,第 7天含量分别是 ( 7.2 7± 2 .2 0 ) μmol/g、( 1.87± 2 .0 1) μmol/g ,第 15天含量分别是 ( 9.3 6± 2 .2 4) μmol/g、( 1.42± 1.2 1) μmol/g ,均较正常对照组显著降低 (P <0 .0 5～ 0 .0 1)。而中药组大鼠第 1天脑海马Glu、Asp含量分别是 ( 9.90± 3 .2 0 )μmol/g、( 2 .95± 1.73 ) μmol/g ,第 7天含量分别是 ( 10 .49± 2 .3 4)μmol/g、( 3 .0 3± 1.0 9) μmol/g ,第15天含量分别是 ( 11.0 5± 2 .5 4)μmol/g、( 3 .0 5± 1.41) μmol/g ,均高于同期模型组 (P <0 .0 5 )。结论 :益肾降浊汤可提高缺血再灌注后脑组织细胞内的EAA含量 ,调节突出间隙EAA浓度 ,对缺血神经元有保护作用。     

QT Measurement: A Comparison of Three Simple Methods

Background: QT interval and QT dispersion are useful noninvasive measurements in clinical cardiology and can be measured by several methods. The comparative variability of these methods, however, is not well defined. Methods: We evaluated the intra- and interobserver variability of three simple methods of QT measurement: (1) ruler method: use of a 0.5-mm scale precision ruler to measure QT with end of T wave determined by extrapolating its slope to baseline; (2) caliper method: use of a standard electrocardiogram (ECG) caliper and the standard ECG paper scale with QT determined by visual inspection; (3) computer method: use of a digitized computer software program with QT determined by cursor set manually by the user. QT intervals from 11 patients (total 44 ECG leads) in sinus rhythm without conduction defect were measured by five blinded, trained observers at two time points (a week apart) in a crossover manner. Results: The mean difference in intraobserver measurements were 6 ± 2, 12 ± 12, and 27 ± 2 ms by the ruler, caliper, and computer methods, respectively (P > 0.01, ruler vs caliper or computer). The mean differences in interobserver measurements were 13 ± 3, 16 ± 4, and 29 ± 3 ms for the same methods, respectively (P > 0.01, ruler vs caliper, computer). Enlargement of the ECG to 200% did not reduce the measurement variability. Conclusion: The ruler method as described yielded the lowest variability in QT measurement.     

Treatment of collagen-induced arthritis with an anti-osteopontin monoclonal antibody through promotion of apoptosis of both murine and human activated T cells

OBJECTIVE: To test the effects of a novel monoclonal antibody (mAb) against human osteopontin (OPN) in the prevention and treatment of collagen-induced arthritis (CIA) and to elucidate the underlying mechanisms of these effects. METHODS: DBA/1J mice immunized with type II collagen to induce CIA were monitored to assess the effects of anti-OPN mAb on the clinical severity of the disease, and pathologic changes in the joints were examined histologically. The effects of anti-OPN mAb on survival of activated T cells from arthritic mice and from the synovial fluid of patients with rheumatoid arthritis (RA) were determined by TUNEL assay or annexin V assay. The levels of apoptosis-related proteins (Bim, Bax, and Bcl-2) and NF-kappaB were detected by immunoblot analysis. RESULTS: One anti-OPN mAb, 23C3, was effective in inhibiting the development of CIA and even reversing established disease in DBA/1J mice. Monoclonal antibody 23C3 reduced the levels of serum type II collagen-specific autoantibodies and proinflammatory cytokines, and suppressed T cell recall responses to type II collagen. Mechanistic studies demonstrated that OPN prevented the death of type II collagen-activated murine T cells and synovial T cells from RA patients. Monoclonal antibody 23C3 promoted apoptosis of the activated T cells, particularly CD4+ T cells, by inhibiting activation of NF-kappaB and by altering the balance among the proapoptotic proteins Bim and Bax and the antiapoptotic protein Bcl-2. Screening of a phage display peptide library led to identification of the epitope ATWLNPDPSQKQ as being recognized by this novel antibody. CONCLUSION: Because of its ability to effectively promote apoptosis of activated T cells, mAb 23C3 may be a novel therapeutic agent for the treatment of RA.     

Anti-SIRT1 autoantibody is elevated in ankylosing spondylitis: a potential disease biomarker

Background

Little is known about the presence of specific autoantibodies in ankylosing spondylitis (AS), an immune-mediated inflammatory disease. The object of this study was to explore potential autoantibody profiles in AS patients.

Results

Levels of anti-SIRT1 autoantibodies were significantly higher in AS (P?< 0.001) and psoriatic arthritis (PsA) (P?<?0.01) patients but not rheumatoid arthritis (RA) patients compared with healthy controls. Additionally, titers of anti-NAD-dependent protein deacetylase sirtuin-1(SIRT1) antibodies were significantly higher in AS patients than in RA (P?<?0.05) and PsA (P?<?0.05) patients. Moreover, levels of anti-SIRT1 (P?<?0.001) antibodies were significantly higher during the first year in patients with hip joint involvement. The anti-SIRT1 antibody positivity rate was 18.9% in AS patients.

Conclusions

Our findings indicate that anti-SIRT1 autoantibodies may serve as a marker for diagnosing AS and predicting hip joint involvement at an early stage.

     

丹皮酚对高脂血清所致大鼠主动脉平滑肌细胞增殖的抑制作用

目的 观察丹皮酚（Ｐａｃ）对大鼠主动脉平滑肌细胞增殖的抑制作用。方法 利用体外细胞培养技术,用人高脂血清造成一滑肌细胞（ＳＭＣ）增殖的模型,并通过ＭＴＴ测定、细胞计数及透射电镜下的细胞超微结构的观察等方法,观察丹皮酚对大鼠主动脉平滑肌细胞的保护高脂血清可明显刺激大鼠主动脉平滑肌细胞的增殖并引起其表型的改变,而Ｐａｃ可呈浓度依赖性地抑制平滑肌细胞的增殖并可显著抑制高强有力血甭对ＳＭＣ的促增殖作用。结     

心肌与血管再生的中西医研究进展

动物实验和初步人体临床试验研究表明,经冠状动脉灌注或心肌直接注射自体干细胞或祖细胞能减轻心肌的疤痕形成和纤维化,增加心肌新生血管的形成达到增加心肌血流量.一些中药也可通过刺激心肌缺血区各种血管生成因子和旁分泌达到促进血管生长的目标.     

老年肺癌组织学类型,分化及P53蛋白表达

目的：研究老年肺癌组织学特点及其Ｐ５３蛋白的表达。方法：肺癌组织学类型按ＷＨＯ标准进行分类，Ｐ５３蛋白的和免疫组化技术。结果：老年组肺癌的组织学类型与非老年组不同，差异有显著性；Ｐ５３蛋白总阳性率为４２．８６％，两组间差异没有显著性。     

70例喉乳头状瘤的外科治疗

目的 喉乳头状瘤是常见的吃部良性肿瘤，本文探讨喉乳头状瘤的外科治疗方式。方法 对１９９０￣１９９８年间７０例喉乳头状瘤不同的外科治疗方式进行性回顾分析。结果 儿童喉乳头状瘤容易复发，蝗喉裂开术均留有喉狭窄后遗症。结论 喉乳头状瘤行手术摘除、激光或微波治疗是比较有效的方式，儿童不适宜作喉裂开术。