膀胱压、胃内压与腹内压的相关性研究

目的 探讨利用膀胱压、胃内压来间接监测腹内压的可行性．以便于腹腔间隔室综合征(ACS)的诊断。方法 对24例行腹腔镜胆囊摘除术(LC)患者进行前瞻性对照研究。在行LC时，在腹内压为10、15、20及25mmHg时同时测定膀胱压和胃内压。结果 腹内压与膀胱压的回归方程为Y=-10.193 1.228X．相关系数为0.941；腹内压与胃内压的回归方程为Y=-7.408 1.478X，相关系数为0.996。由此表明．腹内压与膀胱压和胃内压呈显著正相关。结论 腹内压与胃内压和膀胱压有很好的相关性，可以利用胃内压和膀胱压来反映实际腹内压。     

葛根素双相动态提取工艺优化研究

目的:研究葛根中葛根素的最佳双相动态提取工艺。方法:以葛根素提取率为主要指标,出膏率为次要指标,采用L9(34)正交试验设计对提取工艺进行优化,考查硫酸浓度、溶剂倍数、提取时间、提取次数对葛根素含量的影响。结果:葛根素的最佳提取工艺为浓度2.0%的硫酸,12倍量溶剂1,00℃,提取2次,提取时间1.5 h。结论:优选得到的工艺稳定,重复性好,便于质量控制和工业化生产。     

鼠痘病毒感染机制及其检测方法研究进展

概述了鼠痘病毒(ECTV)入侵动物机体后感染传播的机制,包括小鼠的天然抗病机理、机体免疫应答以及病毒感染的致病机理等。同时总结了检测ECTV的试验动物法、病理学方法、血清学方法和核酸诊断方法等的优缺点。通过系统了解ECTV的感染途径和诊断方法 ,有助于防控ECTV的发生及传播。     

小鼠肝炎病毒N蛋白基因在杆状病毒中的表达

目的 利用Bac-to-Bac杆状病毒表达系统表达小鼠肝炎病毒N基因. 方法 根据GenBank发表的小鼠肝炎病毒A59株N基因序列（AY700211）,设计一对特异性引物,利用RT-PCR方法扩增出N基因的ORF,将目的 片段克隆入pFastBacHTa中,构建重组转移载体pFastBHa-N.将重组质粒转化DH10Bac感受态细菌,与Bacmid发生位点特异性转座作用,获得重组穿梭载体Bacmid-N.通过脂质体将其转染昆虫细胞Sf9. 结果 获得了重组杆状病毒rBN,并通过Westernblot和IFA分析表明证实N蛋白在昆虫细胞中获得正确表达,且表达产物具有抗原性. 结论 利用Bac-to-Bac杆状病毒表达系统成功表达了小鼠肝炎病毒N蛋白,为进一步进行建立诊断学方法的研究奠定了基础.     

急性重症胰腺炎腹内压、腹围与APACHE评分的相关性研究

目的通过对ICU急性重症胰腺炎患者腹内压的监测数据,来推断它在急性重症胰腺炎的严重程度和疗效分析中的临床意义。方法根据我科2005年5月～2009年5月收治急性重症胰腺炎28例（死亡组10例,治愈组18例）的腹内压监测数据,分别比较两组的腹内压监测和腹围与APACHE-II评分的相关性。结果治愈组腹内压与A-PACHE-Ⅱ评分的回归方程是Y=1.406＋0.690X,相关系数r=0.646,P〈0.001,有明显相关性;腹围与APACHE的相关系数r=0.175,P=0.048,有一定相关性。死亡组结果：腹内压与APACHE-Ⅱ评分的回归方程是Y=14.767＋0.471X,相关系数r=0.387,P=0.008,有明显相关性;腹围与APACHE-Ⅱ的相关系数r=0.279,P=0.061没有相关性。结论腹内压监测是急性重症胰胰炎较好的临床疗效评估参数。     

内固定应用于脊柱结核治疗的安全性探讨

Objective To investigate the efficiency and safety of instrumentation to treat spinal tu-berculosis. Methods Fifty-one patients of spinal tuberculosis were treated with one-stage or two-stage de-hridement, strut autografting, and anterior or posterior instrumentation with screw rod system, combined with one-year triple agents postoperative antituberculous chemotherapy from April 1985 to May 2005. There were 6 in cervical spine, 19 in thoracic spine, 16 in thoracolumbar spine and 10 in lumbosacral spine. The level of the lesion were 6 cases for single vertebrae, 30 for two vertebrae, 11 for three vertebrae and 4 for more than four vertebrae. All patients were given triple agents antituberculous chemotherapy at least two weeks before operation. The operative procedures included cervical anterior approaches in 6 cases for debridement, fusion and plate-screw fixation, upper lateral transthoracic procedures for debridement, fusion and screw rod fixation in 11, posterior debridement, fusion and transpedicular screw system fixation in 6, two-stage anterior and posterior operation in 18 cases. The instrumentation included 6 Luque, 3 Z-plate, 8 TSRH, 5 Ventrofix, 4 Kenada, 5 Moss-Miami, 6 Isola, 3 CDH, 2 Caspaz, 2 C-D, 1 Zielke, 1 Dick, 1 Oriell, 1 Ozion, 1 Zephir, 1 Tenor, and 1 USS. Results All patients were followed up prospectively for 3.2 to 23.5 years postoperatively. The back pain was obviously relieved postoperatively. Patients with neurological function deficiency im-proved. The solid fusion was achieved in all patients, except one patient due to tuberculosis recurrence. The major complication included one sinus formation and one case with temporary deterioration of neurological function, recovered with appropriate treatment. Average preoperative kyphosis angle was 34.17°, and that was 10.45° immediately after surgery. There was a 3.2° loss of kyphosis correction during follow-up period. Con-clusion Spinal tuberculosis treated with instrumentation is effective and safe.     

内固定应用于脊柱结核治疗的安全性探讨

Objective To investigate the efficiency and safety of instrumentation to treat spinal tu-berculosis. Methods Fifty-one patients of spinal tuberculosis were treated with one-stage or two-stage de-hridement, strut autografting, and anterior or posterior instrumentation with screw rod system, combined with one-year triple agents postoperative antituberculous chemotherapy from April 1985 to May 2005. There were 6 in cervical spine, 19 in thoracic spine, 16 in thoracolumbar spine and 10 in lumbosacral spine. The level of the lesion were 6 cases for single vertebrae, 30 for two vertebrae, 11 for three vertebrae and 4 for more than four vertebrae. All patients were given triple agents antituberculous chemotherapy at least two weeks before operation. The operative procedures included cervical anterior approaches in 6 cases for debridement, fusion and plate-screw fixation, upper lateral transthoracic procedures for debridement, fusion and screw rod fixation in 11, posterior debridement, fusion and transpedicular screw system fixation in 6, two-stage anterior and posterior operation in 18 cases. The instrumentation included 6 Luque, 3 Z-plate, 8 TSRH, 5 Ventrofix, 4 Kenada, 5 Moss-Miami, 6 Isola, 3 CDH, 2 Caspaz, 2 C-D, 1 Zielke, 1 Dick, 1 Oriell, 1 Ozion, 1 Zephir, 1 Tenor, and 1 USS. Results All patients were followed up prospectively for 3.2 to 23.5 years postoperatively. The back pain was obviously relieved postoperatively. Patients with neurological function deficiency im-proved. The solid fusion was achieved in all patients, except one patient due to tuberculosis recurrence. The major complication included one sinus formation and one case with temporary deterioration of neurological function, recovered with appropriate treatment. Average preoperative kyphosis angle was 34.17°, and that was 10.45° immediately after surgery. There was a 3.2° loss of kyphosis correction during follow-up period. Con-clusion Spinal tuberculosis treated with instrumentation is effective and safe.     

益气固肾汤治疗乳糜尿50例

益气固肾汤治疗乳糜尿５０例李玉杰胡建华白其昌（安徽省阜阳市第一人民医院２３６０００）关键词：益气固肾汤;乳糜尿;尿浊乳糜尿属中医尿浊范畴,目前中西医对此症治疗效果尚不理想。笔者１９８０～１９９５年以自拟益气固肾汤治疗５０例,取得满意的效果。１临床资...     

女性青少年特发性脊柱侧凸患者的椎体形态学测量

突距离进行对比,并进行统计学分析.结果 AIS组10～14岁患者前柱椎体高度大于后柱,椎体前后中心径相对较长,椎体存在楔形变,凸凹侧椎弓根及凸凹侧上下关节突距离均不对称,胸腰椎前后柱的比例明显不同于对照组(P<0.01):而15～18岁患者两组前后柱比例比较差异无统计学意义.结论 AIS椎体冠状面存在楔形变,10～14岁AIS患者椎体高度较高,后方结构相对较小,椎体前后径相对较长,椎体相对细长,这可能是导致侧凸的形成以及发展的原因.     

原发性高血压合并2型糖尿病患者心律失常发病情况分析

目的:探讨原发性高血压(PH)合并2型糖尿病(T2DM)患者心律失常的发生特点。方法:收集PH(及PH合并T2DM)并发心律失常的患者200例,按其是否合并T2DM,将其分为合并糖尿病组(66例)和无糖尿病组(134例),应用统计学方法分析两组病例心律失常构成比的差异。结果:合并糖尿病组心房颤动(房颤)、房室传导阻滞(AVB)发生率较高,而无糖尿病组室性早搏发生率较高。结论:PH合并T2DM时心律失常以房颤最多见,无糖尿病合并症时则以室性早搏最多见。