痰热清注射液对AECOPD患者肺功能和炎症因子的影响

目的探讨痰热清注射液对慢性阻塞性肺疾病急性加重期（AECOPD）患者血浆细胞因子IL-10、C反应蛋白（CRP）及肺功能（FEV1％、FEV1／FVC）的影响,并观察其临床疗效。方法将100例AECOPD患者随机分为两组,均予常规治疗,治疗组加用痰热清注射液;两组疗程均为14d。结果治疗组总有效率高于对照组;治疗组血浆细胞因子IL-10、CRP、FEV1％改善均显著优于对照组。结论痰热清注射液能明显降低AECOPD患者血细胞因子CRP含量,提高血IL-10含量和患者治疗效果,并能显著改善肺功能。     

复感儿GH-IGF-I轴功能水平与正常儿的比较

目的:通过复感儿生长激素(GH)-胰岛素样生长因子I(IGF-I)轴(GH-IGF-I轴)功能水平与正常儿的比较,了解反复呼吸道感染对儿童生长发育的影响.方法:临床确诊的复感儿在非急性感染期采血,采用酶联免疫吸附法(ELISA)测定IGF-I、胰岛素样生长因子结合蛋白-3(IGFBP-3),并与健康儿童组对照.结果:复感儿在非急性感染期GIGF-I、IGFBP-3水平与正常儿相比较,差异有统计意义(P<0.05或P<0.01).结论:复感儿在非急性感染期GH-IGF-I轴功能水平并未受到抑制,相反,与正常儿相比较,复感儿在非急性感染期的生长速度更活跃,有追赶性生长的现象.     

经尿道气化加电切术治疗高危前列腺增生症40例分析

目的:提高经尿道电气化术和电切术治疗高危前列腺增生症(BPH)的安全性和有效性.方法:对40例70～92岁高危BPH患者采用经尿道前列腺电气化加电切术治疗.结果:平均手术时间60 min,无尿失禁和死亡病例,2例输血.术后随访1～12个月,国际前列腺症状评分(IPSS)8分,最大尿流率15 mL·s-1.结论:应用气化切割和电切修切,可以快速去除增生前列腺组织,安全有效,并发症少,值得推广.     

HPLC法测定丹参颗粒中丹酚酸B的含量

目的建立丹参养心颗粒中丹酚酸B含量的测定方法。方法采用HPLC法测定丹酚酸B的含量,色谱柱：OSD C18反相色谱柱（5μm,4.6×250 mm）;流动相：乙腈-2.5%甲酸（20：80）;流速：1.0 ml/min;检测波长：286 nm;柱温：35℃。结果平均回收率为99.55%,RSD%=0.42%。结论方法简便可行,重现性好,可很好地控制丹参养心颗粒的内在质量。     

高浓度氧对早产鼠Ⅱ型肺泡上皮细胞增殖和细胞周期的影响

目的探讨高浓度氧（简称高氧）对早产鼠Ⅱ型肺泡上皮细胞（AECⅡ）增殖和细胞周期的影响。方法原代培养早产大鼠AECⅡ，建立高氧细胞损伤模型。血球计数板计数法对培养细胞计数，台盼蓝拒染法检测细胞活力，流式细胞术检测细胞周期和Ki67表达。结果空气组AECⅡ在培养后其数目不断增加，高氧组给氧后48和72h，细胞数目减少、细胞活力降低。高氧使G0／G1期细胞比例显著增多，S和G2／M期细胞比例明显减少。高氧组给氧后24、48及72h，Ki67阳性细胞的表达率及荧光指数较同时间点空气组均明显降低（P〈0．05或P〈0．01）。结论原代培养的早产大鼠AECⅡ暴露在高氧环境中发生G1期阻滞，Ki67表达减少，细胞增殖受抑，这种增殖受抑与早产儿慢性肺损伤的发生密切相关。     

新生儿ABO血型鉴定及与异型血交叉配血结果分析

<正>人类ABO血型系统抗原在胚胎早期(约37d)就开始发育,但其抗原强度直至出生也不一定明显增加,自身ABO血型抗体也尚未完全产生(出生3~6个月后逐渐产生合成),故新生儿ABO血型血清学检测有其独特性。本文回顾性分析我院部分新生儿ABO血型鉴定及异型交叉配血结果。1临床资料2009年1月-2012年12月于泸州医学院附院住院的新生儿1 095例,日龄1~28 d,其中早产儿153例,足月儿908例,过期儿34例。男645例,女450例。所有新生儿和献血者标本不规则抗体筛查均为阴性。血型鉴定卡、交叉配血卡及不规则抗体筛查卡均由丹麦戴安娜公司提供;抗体筛查细胞由瑞士达亚美公司提供。所有操     

环戊硫酮治疗干眼的临床疗效评价

Objective To investigate anethol trithione therapic efficiency on dry eye. Methods It was a prospective random double-blind controlled study. Eighty cases diagnosed dry eye in Ocular Surface Out-patient Clinic of Xiamen University Affiliated Xiamen Eye Center from 2006 to 2008 were divided into two groups: anethol trithione group and control group, 40 cases in each group. Every group was then divided into two subgroups: weak dry eye subgroup, middle and severe dry eye subgroup. All groups had been added with 0.05% refresh drops. All patients had been detected and evaluated by subjective symptoms of dry eye, visual acuity,corneal fluorescent staining(F1), break-up time(BUT) and Schirmer Ⅰ test (SⅠT) at pre-therapy and 3,7,28 d of post-therapy. All groups had been compared and analyzed by F test and sample mean difference (SMD) or median difference (MD) comparison between pre-therapy and post-therapy. Results Except of tear and red eye, the other subjective symptoms of dry eye, F1, BUT and SⅠT of weak dry eye subgroup of both groups had been improved at 7 d after therapy. Only those of middle and severe dry eye subgroup of anethol trithione group had been improved at 7 d after therapy compared with those of pre-therapy: SMD=0.96 (visual tiredness), 1.26 (dry and unsmooth sensation), 0.82 (foreign body sensation), 1.28 (burning sensation), 1.05 (photophobia), 1.48 ( pain ) ; MD=0.30 (visual acuity), 4.00 (F1) ,5.00 (BUT), 5.00 (SⅠT) [F=15.30 (visual tiredness), 15.68 (dry and unsmooth sensation ), 13.56 (foreign body sensation), 20. 91 ( burning sensation ), 18.90 (photophobia), 27.22 ( pain ), 10.54 (visual acuity), 188.21 (F1) ,261.76 (BUT) ,269.05 (SⅠT) ;P<0. 05]. Those of middle and severe dry eye subgroup of control group hadn't significantly been improved at 28 d after therapy: SMD=0.10 (visual tiredness) ,0.16 (dry and unsmooth sensation) ,0.09 (foreign body sensation) ,0.38 ( burning sensation ), 0.24(photophobia) ,0.36 (pain) ,0.23 (red eye) ; MD=0.10 (visual acuity) ,0.50(F1) ,0.50(BUT), 0.50(SⅠT) [F=1.76 (visual tiredness), 1.61 (dry and unsmooth sensation), 1.02 (foreign body sensation),2.39 (burning sensation), 2.42 (photophobia), 2.73 ( pain ), 2.55 ( red eye ), 1.46 ( visual acuity) ,2.35 (F1) ,2.90 (BUT) ,2.76 (SⅠT) ; P>0.05]. SⅠT of anethol trithione group had been improved more significantly after therapy (F=13.77, P<0.05). Conclusion Anethol trithione could significantly improve middle and severe dry eye patients' symptoms and signs whose lacrimal gland function survival and it has clinical application value.     

LASIK术后非结核分枝杆菌性角膜炎1例

0引言随着激光原位角膜磨镶术(LASIK)手术的广泛开展,LASIK术后并发症也在增加,术后感染性角膜炎的发病率为0.1%~0.2%,而真实的发病率可能远超过所报道过的病例[1]。我们在LASIK术后3d发现1例非典型性分枝杆菌性角膜炎,现报告如下。1病例报告患者,男,35岁,因双眼视物不清20a+于2008-03-16在外院行LASIK手术,术后常规使用糖皮质激素滴眼。术后第1d复查,裸视力右1.2,左1.0,无不适感,术后检查未发现异常;第3d双眼轻度畏光,流泪,右眼角膜未见异常,左眼视力明显下降,裂隙灯检查显示:角膜水肿,角膜瓣下有散在圆点状上皮下浸润,边界不清(图1);第4d,双眼畏光     

ICL植入术矫正超高度近视的临床效果观察

目的:评估有晶状体眼后房型人工晶状体(ICL)矫正超高度近视的疗效及安全性。方法:对超高度近视患者20例40眼接受普通型ICL或散光型后房型人工晶状体(TICL)治疗,术前屈光度球镜-10.0～-18.0D,柱镜-0.25～-3.00D,等效球镜-15.32D,术后随访12mo,观察指标包括UCVA,BCVA、角膜地形图、主观和客观验光、角膜内皮细胞计数、眼压测量、裂隙灯检查。结果:术后12mo,裸眼视力≥0.8者占80.0%(32眼)。30.0%(12眼)的术眼BCVA较术前提高1行,15.0%(6眼)的术眼BCVA较术前提高2行。术后12mo术眼屈光度在±0.50D者达70.0%(28眼)。术前患者平均眼压为16±2.8mmHg,术后6mo平均眼压为17±3.4mmHg,术前术后相比较差异无显著性(t=0.518,P=0.776),术前患者角膜内皮细胞计数平均为2823±243.6个/mm2,术后6mo平均为2709±273.2个/mm2,术前术后比较无显著性差异(t=0.794,P=0.422)。未发生继发性青光眼、视网膜脱离及并发性白内障。结论:ICL植入术矫正超高度近视具有良好的疗效和安全性,远期效果有待进一步观察。