不稳定性心绞痛及急性心肌梗塞病人血浆PAI—1活性的观察

对ＵＡ和ＡＭＩ病人血浆ＰＡＩ－１活性水平及其动态变化进行了观察研究。结果显示：血浆ＰＡＩ－１活性ＵＡ组（９３２±２.６０ＡＵ／ｍｌ）及ＡＭＩ组（1０.２±１.５９ＡＵ／ｍｌ）均高于对照组（６.７５±２.６０ＡＵ／ｍｌ）（Ｐ＜０.０５）；ＡＭＩ病人血浆ＰＡＩ－ｌ活性在治疗早期有一过性下降，以后均高于正常水平。     

山莨菪三防治筋膜间室综合征实验研究

筋膜间室综合征颇常见,它可严重影响肢体功能,甚至威胁生命。手术切开方法确能有效地防治筋膜间室综合征,但有不少缺点,如加重原有创伤,增加感染机会,使伴有的骨折处理更加困难,创口难以愈合,常需二期处理,疤痕影响美观,降低肌肉功能等,对可疑的和室压介于争议区病例确定是否手术亦较难。所以近年来非手术治疗方法有所发展,杨迪生等报道了山莨菪硷合并甘露醇治疗筋膜间室综合征获得一定效果⁽¹⁾,本研究是参照Sheridan筋膜间室综合征兔模型⁽²⁾,通过动物实验观察山莨菪碱防治筋膜间室综合征的作用及机制。     

活血化瘀等中药对培养心肌细胞搏动的影响

实验表明:青皮、附子、鸡血藤、川芎、麝香、益母草、红花、丹皮、赤芍、丹参、刘寄(?)、郁金等以活血化瘀为主的十二味药物,对培养心肌细胞搏动节律的影响,可分为加快、减慢和不影响节律三种情况。本文分别比较了以上不同药物的作用强弱,讨论了几种药物对心肌细胞α、β受体的影响及毒性,为临床选用这些药物提供参考。     

血管内皮细胞对增生性瘢痕成纤维细胞增殖的影响

目的：观察人脐静脉血管内皮细胞对增生性瘢痕成纤维细胞增殖活性的影响。方法：收集培养的人脐静脉血管内皮细胞上清条件培养液，加入培养的增生性瘢痕成纤维细胞之中，采用MTT法检测增生性瘢痕成纤维细胞增殖率，利用流式细胞仪分析细胞周期。结果：加入内皮细胞上清液的增生性瘢痕成纤维细胞增殖比对照组明显增加，且S期比例增高。结论：在瘢痕形成的过程中，内皮细胞被激活，激活的内皮细胞可能分泌某些活性物质，促进成纤维细胞增殖，刺激其进入细胞DNA合成期S期。     

新疆不同民族食管癌血浆代谢组学差异分析

Objective To investigate the metabonomic variation between patients with esophageal cancer (EC) and healthy controls, and to analyze the variation between patients with EC. Methods 1H-MR and orthogonal partial least-squares discriminant analysis (OPLS-DA) was performed on 108 plasma specimens from EC patients and 50 health controls, and the metabonomic variation between patients with EC and healthy controls analyzed. Results OPLS-DA analysis might correctly separate all plasma specimens from health controls and patients with EC ,leucine(0. 0043±0. 0006,0. 0040±0. 0006) ,alanine(0. 0039±0. 0007,0. 0033±0. 0006), isoleucine (0. 0067±0. 0010,0. 0063±0. 0009), valine (0. 0037±0. 0005,0. 0035±0. 0006) ,glycopretein(0. 0123±0. 0043,0. 0102±0. 0022), lactate(0. 0342±0. 0113,0. 0258±0. 0085), acetone (0. 0027±0. 0023,0. 0017±0. 0008), acetate (0. 0007±0. 0001,0. 0006±0. 0001),choline(0.0035±0.0006, 0.0029±0.0007), isobutyrate (0.0020±0.0004, 0.0018±0.0003),unsaturated lipid (0. 0072±0. 0013,0. 0059 + 0. 0018), VLDL (0. 1209±0. 0589,0. 0879±0. 0269), LDL (0. 0885±0. 0328,0. 0785±0. 0288), 1-methylhistidine(O. 0005±0. 0001,0. 0004±0. 0005)decreased in EC patient' s plasma with statistical significance (r total>0. 27, P<0. 05), dimethylamine (0. 0004±0. 0001,0. 0005±0. 0001), α-glucose (0. 0079±0. 0013,0. 0081±0. 0016), β-glucose (0. O139±0. 0024,0. 0142±0. 0029) ,citric acid (0. 0044±0. 0008,0. 0106±0. 0058) increase in the EC patient's plasma (r total<-0. 27 ,P<0. 05). There were clear variation between Han and Kazak patients,alanine(0. 0031±0. 0005,0. 0029±0. 0004), glutamine (0. 0010±0. 0001,0. 0009±0. 0001), tyrosine (0. 0009±0. 0001,0. 0008±0. 0001), 1-methylhistidine (0. 0005±O. 0001,0. 0004±0. 0001) increased in the Han patients (r>0. 35, P>0. 05), carnitine (0. 0028±0. 0006) was higher in Kazak patients than in Han patients (0. 0025±0. 0004), which had statistical siginificance (r=-0. 40, P<0. 05). Unsaturated lipid (0. 0059±0. 0018,0. 0047±0. 0011), isoleucine (0. 0062±0. 0011,0.13058±0. 0007), alanine (0.0032±0. 0007, 0. 0028±0. 0004) ,glycoprotein (0. 0096±0. 0019,0. 0086±0. 0011) ,glutamine (0. 0011±0. 0001,0. 01309±0. 0001) ,tyrosine(0. 0009±0. 0001, 0. 0008±0. 0001), 1-methylhistidine (0. 0005±0. 0001, 0. 0004±0. 0001)were increased in Uygnr patients as compared with Kazak patients, having statistical significance (r>0. 33 ,P<0. 05), carnifine (0. 0027±0. 0005) was higher in Kazak patients than in Uygur patients (0.0025±0.0004)(r=-0.36,P<0.05). Conclusion The results indicate that1H-MR spectra of plasma analyzed by OPLS-DA statistical methods might completely separate the EC patients from health controls. The metabonomic approach should be helpful in screening of EC patients.     

尿路上皮肿瘤患者外周血Uroplakin Ⅱ基因检测的意义

目的　探讨尿路上皮特异性跨膜蛋白UroplakinⅡ (UP Ⅱ )表达在尿路上皮肿瘤血行播散及判断化疗效果中的意义。　方法　采用RT PCR法检测 5 4例尿路移行上皮癌 (TCC)患者外周血标本UP Ⅱ表达 ,并对 4例接受化疗的患者进行追踪检测。　结果　表浅肿瘤患者外周血UP ⅡmRNA无阳性表达 ,浸润性肿瘤患者阳性表达率 2 0 .8% (5 / 2 4 ) ,局部淋巴结转移者 2例中阳性表达 1例 ,有远处转移者 2例均阳性表达。 7例接受化疗者治疗前UP Ⅱ均呈阳性表达 ,2～ 3个疗程后 3例转阴。　结论　UP Ⅱ检测对于判断尿路上皮肿瘤的血行播散、转移及治疗反应有重要意义。     

过滤型高分子季铵盐消毒剂的研究

过滤型高分子季铵盐消毒剂[1、2、3]是强碱性阴离子交换树脂的三碘化合物,是一类新型的交联不溶性消毒剂。它们遇到细菌能释放出杀菌物质,能广谱杀菌和杀病毒,可以用作饮用水和工业用水的消毒剂。这类消毒剂具有杀菌效率高,经其处理过的水中不含残留药物,并且水的色、味、嗅不受影响等优点,比常用的饮水消毒剂优越。Taylor报道[3]Ionac