B超在使用自凝刀治疗子宫肌瘤中的监护作用

我院2002年5月～2004年5月在B超监护下经阴道用自凝刀射频治疗子宫肌瘤、子宫腺肌瘤87例，效果满意，现报告如下。     

泽泻有效部位对肾草酸钙结石模型大鼠肾组织骨桥蛋白表达的影响

目的研究泽泻有效部位对大鼠肾草酸钙结石形成和肾组织骨桥蛋白(OPN)表达的影响,探讨泽泻抑制尿结石形成的机制。方法采用现代植化和生物活性导向分离的方法分离、提取泽泻的有效部位。将30只大鼠随机分成3组对照组、模型组、泽泻组。以乙二醇和1α-羟基维生素D3ig制备大鼠肾草酸钙结石模型。检测各组大鼠血及尿生化、肾Ca2 水平、24h尿草酸分泌量和肾组织病理学改变,并采用免疫印迹(Western blotting)和半定量逆转录聚合酶链反应(RT-PCR)分别观察各组大鼠肾组织OPN蛋白及其mRNA的表达水平。结果ig泽泻有效部位(主要以四环三萜类化合物为主)大鼠的血清肌酐(SCr)、尿素氮(BUN)、肾Ca2 水平、24h尿Ca2 分泌量、肾组织草酸钙晶体的分布、OPN蛋白及其mRNA的表达水平均显著低于模型组(P<0.01)。结论泽泻有效部位能抑制大鼠肾组织OPN的表达,减少肾组织草酸钙结晶的沉积,从而能有效抑制大鼠肾草酸钙结石的形成。     

微创经皮肾镜治疗上尿路结石并感染

目的 探讨微创经皮肾镜治疗榆尿管上段结石及肾结石并感染的疗效.方法 对感染较轻的肾、输尿上段结石并感粢者,经控制感染后Ⅰ期行微创经皮肾镜术9例;合并较严重感染者先期行经皮肾造痿、Ⅱ期微创经皮肾镜碎石取石术32例.结果 Ⅰ期行微创经皮肾铰碎石取石术9例中,2例术后出现高热(＞39℃).2例术后体温为38-390C,其余5例术后体温＜38℃.Ⅱ期经皮肾镜碎石取石术32例.所有病例Ⅱ期经痿道碎石取石术后体温均＜38℃.结论 对于榆尿管上段及肾结石并感染患者,经抗炎治疗后复查,血象、体温正常、无腰痛、估计手术时间不长者,选择Ⅰ期经皮肾镜碎石取石术;反之,选择Ⅱ期经皮肾镜术.     

右心房转移性子宫平滑肌肉瘤1例

心脏转移性肿瘤较少见，而子宫平滑肌肉瘤转移至心脏尚未见报道。本科于2007年11月收治右心房转移性子宫平滑肌肉瘤患者1例，报道如下。     

A Comparative Study on Several Models of Experimental Renal Calcium Oxalate Stones Formation in Rats

In order to compare the effects of several experimental renal calcium oxalate stones formation models in rats and to find a simple and convenient model with significant effect of calcium oxalate crystals deposition in the kidney, several rat models of renal calcium oxalate stones formation were induced by some crystal-inducing drugs (CID) including ethylene glycol (EG), ammonium chloride (AC), vitamin D3 [1α(OH)VitD3, alfacalcidol], calcium gluconate, ammonium oxalate, gen-tamicin sulfate, L-hydroxyproline. The rats were fed with drugs given singly or unitedly. At the end of experiment, 24-h urines were collected and the serum creatinine (Cr), blood urea nitrogen (BUN), the extents of calcium oxalate crystal deposition in the renal tissue, urinary calcium and oxalate ex-cretion were measured. The serum Cr levels in the stone-forming groups were significantly higher than those in the control group except for the group EG L-hydroxyproline, group calcium gluconate and group oxalate. Blood BUN concentration was significantly higher in rats fed with CID than that in control group except for group EG L-hydroxyproline and group ammonium oxalate plus calcium gluconate. In the group of rats administered with EG plus Vitamin D3, the deposition of calcium ox-alate crystal in the renal tissue and urinary calcium excretion were significantly greater than other model groups. The effect of the model induced by EG plus AC was similar to that in the group in-duced by EG plus Vitamin D3. EG plus Vitamin D3 or EG plus AC could stably and significantly in-duced the rat model of renal calcium oxalate stones formation.     

泽泻活性成分对肾结石模型大鼠bikunin表达的影响

目的研究中药泽泻活性成分对结石模型大鼠肾结石形成和bikunin表达的影响,以探讨泽泻防治草酸钙尿石症的作用机理.方法分离提取泽泻的化学活性成分,制作大鼠肾草酸钙结石模型.将Wistar大鼠随机分成对照组、结石模型组、泽泻组,采用半定量逆转录聚合酶链反应检测大鼠肾组织bikunin mRNA的表达水平、镜下观察肾组织草酸钙晶体分布,同时检测大鼠血生化、肾钙含量、24h尿钙和尿草酸的分泌量.结果①泽泻组大鼠肾组织草酸钙晶体分布、血生化等指标均明显低于结石组.②泽泻组和成石组大鼠肾组织bikunin的相对表达水平分别为(0.528±0.170,0.713±0.250)、肾钙含量分别为[(4.70±0.08)mg/g,(9.49±0.45)mg/g]、24h尿钙分泌量分别为[(37.23±1.84)μmol,(61.49±2.06)μmol],各组间差异均有显著性意义(P＜0.05).结论泽泻活性成分能下调bikunin在结石大鼠肾组织的表达,减少肾组织草酸钙晶体的形成,从而抑制大鼠肾结石形成.     

留住青春，女人从30开始

幸福是什么？在林林总总的回答中，大多数人把“健康”因素放在首要位置，是呀！如果连个好身体都没有，怎么去“快乐工作，享受生活”呢？专家建议，女人从30岁开始，就应该关注自己，每年到医院体检一次，让自己的一生都在健康中度过。[编者按]     

黄芪与刺五加水提物对弱精子症患者精子体外运动参数的影响

目的研究黄芪和刺五加水提物对弱精子症患者体外精子运动活力的影响。方法将30例弱精子症患者的精子经上游优化法处理后分为8份,其中两份分别加入刺五加水提物和黄芪水提物,使其终浓度分别为10和20mg.mL-1;另取5份将10 mg.mL-1刺五加水提物和20 mg.mL-1黄芪水提物按体积比为4:1,2:1,1:1,1:2和1:4加入;另外1份加入Ham’s F10培养液作对照。分别于孵育15,30和60 m in后应用计算机辅助的精子分析系统(CASA)观察精子各运动参数。结果刺五加水提物和黄芪水提物联用能不同程度地提高弱精子症患者精子的体外运动能力。加入刺五加和黄芪水提物后,精子活率(Mot)、前向运动精子百分率[(A+B)%]、曲线运动速度(VCL)、直线运动速度(VSL)和平均路径速度(VAP)均较对照组显著提高(均P<0.05)。10 mg.mL-1刺五加水提物和20 mg.mL-1黄芪水提物体积比为1:1,共同孵育60 m in作用效果最明显。结论刺五加和黄芪水提物联用能明显改善弱精子症患者精子的体外运动能力。     

Expression of survivin and E-cadherin in breast cancer

Survivin is a member of the inhibitor of apoptosis (IAP) family, and is involved in the regulation of cell division. Survivin expression is related with poor survival of patients with tumors including neuroblastoma, non-small-cell lung cancer, breast carcinoma, gastric carcinoma, rectal cancer, recurrent ????????????colorectal carcinoma and bladder cancer. It is thought to be an important prognostic marker in cancers[1,2]. E-cadherin functionally belongs to transmembrane glycoproteins family,…     

糖化终末产物受体在大鼠胰腺星形细胞中的表达

目的研究胰腺星形细胞(PSCs)是否表达糖化终末产物受体(RAGE),并探讨糖化终末产物(AGEs)对RAGE表达的调节作用。方法分离培养SD大鼠PSCs,采用半定量逆转录聚合酶链反应(RT-PCR)和免疫细胞化学检测静息和活化的PSCs RAGE在mRNA和蛋白水平的表达,同时用RT-PCR检测AGEs刺激后RAGE表达的变化。结果静息和活化的PSCs均表达RAGE,且活化后表达水平提高;AGEs能上调RAGE的表达。结论静息和活化的PSCs均表达RAGE,活化的PSCs RAGE表达上调,提示PSCs是AGEs作用的靶细胞。