山丘型血吸虫病疫区监测巩固对策及结果分析

山丘型血吸虫病疫区监测巩固对策及结果分析湖北省荆门市地方病防治办公室丁兆军荆门市是一个山丘型血吸虫病流行区，历史累计钉螺面积ｉ０６３．７３万ｍ２，查出病人７７５３人。１９９０年达到消灭血吸虫病标准。１９９１年根据湖北省制订的《消灭血吸虫病地区巩固监测...     

江汉航道对沙洋及汉水地区血吸虫病传播影响探讨

目的　探讨江汉航道工程对沙洋及汉水地区血吸虫病传播的影响。方法　在江汉航道沿岸的沙洋段选择一定数量的居民和流动人口采用间接血凝试验(IHA)和粪检进行查病,并选择2个螺情监测点进行螺情调查。结果　河道上游居民血吸虫IHA≥1∶10达到3.89% ,流动人口血吸虫感染率达到0 .6 3% ,无钉螺分布;下游(紧靠沙洋段河道)钉螺面积16 9.92 hm2 ,钉螺感染率0 .13% ,人群感染率4 .95 %。河道钉螺距沙洋最近处2 .5 km。结论　如不采取有效措施,下游钉螺可能通过航道进入沙洋,可引发沙洋及汉水地区血吸虫病的传播流行。要设立监测点,加强监测。     

神经生长因子及其受体在人胰腺导管癌组织中的表达

Objective To investigate nerve growth factor (β-NGF) and its receptors expression in human pancreatic ductal adenocarcinoma. Methods Expression and distribution of β-NGF, tyrosine kinase A (TrKA) and P75NGFR were detected in operation tissue specimens of pancreatic ductal adenocarcinoma with immunohistochemistry and real-time PCR. Relations of β-NGF and its receptors with clinicalpathological characters, especially nerve invasion were analyzed. Results β-NGF and TrKA expression are higher in pancreatic adenocarcinoma than normal pancreas, and the differences are significant (P < 0. 01). Β-NGF and TrKA expression are associated with the differentiation grades(DG), lymphatic node metastasis, nerve invasion and surgical pathological stages. Poorer of DG and later stages, more expression of β-NGF and TrKA. Β-NGF and TrKA expression have positive correlations. Β-NGF, TrKA and P75NGFR mRNA expression have significantly increased 3.84,4. 23 and 2. 41 times than normal tissues by real-time PCR, respectively. Conclusions β-NGF and TrKA might play potential rules in carcinogenesis for pancreatic cancer,have affinity with clinicopathological characters of pancreatic cancer. Β-NGF and TrKA may have mutual effect in signal transduction leading to perineural invasion of pancreatic carcinoma.     

南水北调中线引江济汉水利工程对血吸虫病流行的影响趋势Ⅲ2007年工程区疫情现状

目的了解南水北调中线引江济汉水利工程对血吸虫病流行的影响,为制定防治对策提供依据。方法制定统一的调查方案,2007年调查工程区内的荆州、沙洋、潜江3个县（市、区）的血吸虫病疫情。结果2007年工程区域内现有钉螺面积48.54 hm^2,慢性血吸虫病人251例。结论工程区钉螺面积、钉螺密度逐年下降。工程区存在着血吸虫病流行的潜在威胁。     

卒中型脑瘤30例临床分析

我院自1989年7月至2002年7月收治30例卒中型脑瘤病人,现就其发病特点、诊断治疗及预后情况分析如下：     

沙洋县历史非流行区1起输入性血吸虫病疫情处理效果

2006年沙洋县发生1起输人性血吸虫病疫情,经疫情监测、传染源控制及健康教育等综合防治后,疫情得到有效控制。     

前列腺干细胞抗原在人胰腺癌神经浸润转移中作用的研究

目的探讨前列腺干细胞抗原(PSCA)在人胰腺癌神经浸润中的作用.方法80例胰腺癌手术标本切片行HE染色,观察记数小血管、小淋巴管、神经纤维浸润数.用鼠抗人PSCA单抗行免疫组化(Elivison二步法)染色,观察肿瘤细胞的阳性情况.结果肿瘤细胞神经浸润阳性与小血管、小淋巴管的浸润呈正相关(P<0.01);肿瘤细胞PSCA表达阳性(53例)与神经浸润阳性(66例)呈正相关(P<0.01);肿瘤的恶性程度与PSCA表达呈正相关;PSCA表达与小血管、小淋巴管浸润无关.结论PSCA可能是胰腺癌的特征性分子之一,可引导胰腺癌细胞向神经纤维趋化和黏附,即起"导航"和"停泊"作用.     

前列腺干细胞抗原在人胰腺癌神经浸润转移中作用的研究

目的探讨前列腺干细胞抗原(PSCA)在人胰腺癌神经浸润中的作用.方法80例胰腺癌手术标本切片行HE染色,观察记数小血管、小淋巴管、神经纤维浸润数.用鼠抗人PSCA单抗行免疫组化(Elivison二步法)染色,观察肿瘤细胞的阳性情况.结果肿瘤细胞神经浸润阳性与小血管、小淋巴管的浸润呈正相关(P<0.01);肿瘤细胞PSCA表达阳性(53例)与神经浸润阳性(66例)呈正相关(P<0.01);肿瘤的恶性程度与PSCA表达呈正相关;PSCA表达与小血管、小淋巴管浸润无关.结论PSCA可能是胰腺癌的特征性分子之一,可引导胰腺癌细胞向神经纤维趋化和黏附,即起"导航"和"停泊"作用.     

Artemin及受体GFRα3对MIA PaCa-2人胰腺癌细胞浸润和转移能力的影响

目的 探讨Artemin及其受体GFRα3对MIA PaCa-2人胰腺癌细胞浸润转移能力的影响.方法 以MIA PaCa-2人胰腺癌细胞为研究对象,采用Transwell Chamber分析不同浓度的Artemin及受体GFRα3对胰腺癌细胞浸润转移能力的影响,MIA PaCa-2胰腺癌细胞经Artemin及受体GFRα3刺激后,采用RT-PCR及Western blot方法定量分析MIA PaCa-2细胞中基质金属蛋白酶-2（MMP-2）、上皮钙粘附素（E-cadherin）表达水平的变化.结果 随着Artemin及受体GFRα3作用浓度的增加,MIA PaCa-2胰腺癌细胞浸润转移能力明显增强,均明显高于对照组[150 ng/ml浓度：Artemin组：107.4±11.4;GFRα3组：94.4±9.3;对照组：34.6±7.3,P〈0.01].150 ng/ml为二者的最佳作用浓度.经150 ng/ml Artemin、GFRα3分别作用后,MIA PaCa-2细胞合成MMP-2明显增加[Artemin组：（2.17±0.05）×108;GFRα3组：（2.02±0.03）×108;对照组：（1.02±0.02）×108,t=6.35,7.32],而E-cadherin明显降低[Artemin组：（0.65±0.04）×108;GFRα3组：（0.74±0.01）×108;对照组：（1.36±0.03）×108,t=4.27,5.61],与对照组比较差异均有统计学意义（P〈0.01）.结论 Artemin及其受体GFRα3可促进胰腺癌细胞的浸润和转移能力,可能与浸润转移相关分子MMP-2表达上调、E-cadherin表达下调有关.     

Effects of Artemin and its receptor GFRα - 3 on the invasion and metastasis of human pancreatic cancer cell line MIA PaCa-2

Objective To investigate the effects of artemin and its receptor GFRα - 3 on the invasion and metastasis of pancreatic cancer cells. Methods Human pancreatic cancer cell line MIA PaCa -2 was used in this study. Transwell cell culture chamber assay in vitro was used to detect the ability of invasion and metastasis of MIA PaCa -2 cells. The influence of artemin and GFRα -3 on the protein expression of MMP-2 and E-cadherin was investigated by Western blot and quantitative real time polymerase chain reaction-analyses (Q-RT-PCR). Results As the increase of artemin and GFRα -3, the invasion and metastasis of MIA PaCa- 2 was markedly increased [ 150 ng / ml concentration: Artemin group: 107.4 ± 11.4;GFRα3 group:94. 4 ± 9. 3 ;control group:34. 6 ± 7. 3, P < 0. 01 ]. With 150 ng / ml artemin and GFR-3,the synthesis of MMP-2 in MIA PaCa 2 cells was significantly increased than that in control group[ Artemin grou: (2. 17 ± 0. 05 ) × 108; GFRα3 group: (2. 02 ± 0. 03 ) × 108; control group: ( 1.02 ± 0. 02 ) × 108, t =6. 35,7. 32 ], while E-cadherin significantly decreased [ Artemin group: ( 0. 65 ± 0. 04 ) × 108; GFRα3 group: (0. 74 ± 0. 01 ) × 108; control group: ( 1. 36 ± 0. 03 ) × 108, t = 4. 27,5.61 ], the difference was statistically significant ( P <0. 01 ). Conclusions Artemin and its receptor GFRα3 could promote pancreatic cancer cell invasion and metastasis. This effect may be related to the up-regulated expression of MMP-2 and down regulated expression of E-cadherin.