Durable Mesenchymal Stem Cell Labelling by Using Polyhedral Superparamagnetic Iron Oxide Nanoparticles

Small polyhedral superparamagnetic iron oxide (SPIO) nanoparticles (<10 nm) coated with a thin layer of silica were prepared (SPIO@SiO₂ and SPIO@SiO₂‐NH₂). Surface modification of the small polyhedral silica‐coated SPIO nanoparticles with amines led to substantially higher mesenchymal stem cell (MSC) labelling efficiency without the use of additional transfecting agents. Therefore, amine surface‐modified nanoparticles (SPIO@ SiO₂‐NH₂) appeared to be the preferred candidate for MSC labelling. In vitro studies demonstrated that controlled labelling of SPIO@SiO₂ and SPIO@SiO₂‐NH₂ did not cause MSC death or proliferation inhibition. MSCs labelled with SPIO@SiO₂‐NH₂ nanoparticles retained differentiation potential and showed osteogenic, adipogenic and chondrogenic differentiations. The noncytotoxic polyhedral SPIO@SiO₂‐NH₂ nanoparticle‐labelled MSCs were successfully implanted in rabbit brain and erector spinae muscle, and demonstrated long‐lasting, durable MRI labelling efficacy after 8–12 weeks.     

基于平板振动精确化方程求解动应力集中问题

基于文献[8]给出的平板弯曲振动精确化方程,对含圆孔平板中弹性波散射与动应力集中问题进行了研究.文中给出了分别基于Mindlin板与精确化板方程在不同参数下圆孔动弯矩集中系数的数值结果,并对结果进行了对比分析和讨论.结果表明:在较低频率和薄板情况下,基于文献[8]的方程与基于Mindlin板理论得到的动弯矩结果是基本一致的;在较高频率和厚板情况下,基于文献[8]的方程与基于Mindlin板理论的动弯矩结果相差较大,最大值超出可达16%.由于文献[8]给出的平板振动精确化方程是在没有任何工程假设条件下得到的,因此其分析计算结果更精确一些.      

Scattered waves and motions of marine vessels advancing in a seaway

The steady forward motion of a marine vessel modifies the incident and scattered waves as well as its dynamic characteristics in a seaway. Small amplitude assumptions of the surface waves and vessel dynamic responses lead to linearization of the potential flow problem and its solution in the frequency domain. The mathematical formulation adopts a translating coordinate system at the vessel forward speed. The free surface boundary condition accounts for the modification of the uniform current around the vessel and a new radiation condition takes into account the Doppler shift of the scattered waves. A boundary element model, based on the Rankine source distribution, describes the steady and oscillatory flows around the vessel. Stokes’ theorem allows evaluation of the surface integrals involving the so-called m-terms due to oscillation of the vessel in a current. Through a numerical experiment with a Wigley hull form, we establish the convergence of the numerical model, verify the radiation condition, and examine the scattered wave patterns for a full range of forward speeds. Previous laboratory data provides validation of the computed hydrodynamic coefficients and dynamic response as well as the potential flow model for general seakeeping applications.     

Exceptionally strong multiphoton-excited blue photoluminescence and lasing from ladder-type oligo(p-phenylene)s

We report the synthesis and investigation of multiphoton absorption properties of a novel series of diphenylamino-end-capped ladder-type oligo(p-phenylene)s which exhibit greatly enhanced and efficient multiphoton (from two- to five-photon) upconverted blue photoluminescence with which the record-high intrinsic three-photon absorption cross-section of 4.56 × 10(-76) cm(6) s(2) in the femtosecond regime has been obtained. Exceptionally efficient two- to five-photon-excited lasing in the blue region has also been demonstrated in which the highest two-photon-excited lasing efficiency of 0.34% has been achieved.     

Poitou–Tate duality over extensions of global fields

In this paper, we are interested in the Poitou–Tate duality in Galois cohomology. We will formulate and prove a theorem for a nice class of modules (with a continuous Galois action) over a pro-p ring. The theorem will comprise of the Tate local duality, Poitou–Tate duality and the Poitou–Tate?s exact sequence.     

1.54 and 1.75 μm infrared luminescence of Y2O3:Er

A new 1.75 μm infrared emission transition of Y₂O₃:Er³⁺ is assigned to the ⁴S_3/2 → ⁴I_9/2 transition of Er³⁺ ions situated at the C₂ sites of cubic RE₂O₃ (RE = Y, Gd, Lu). The intensities of features in the 1.54 μm ⁴I_15/2–⁴I_13/2 absorption transition due to Er³⁺ at S₆ and C₂ sites are consistent with the site occupation ratio and the relative magnetic dipole–electric dipole intensity contributions of Er³⁺ at the different sites. The 1.54 μm emission lines are predominantly from Er³⁺ ions at C₂ sites. The different behaviours of the emission intensities 1.75 and 1.54 μm groups with change in Er³⁺ dopant ion concentration, preparation technique, Yb³⁺ co-doping, temperature change and different excitation line are rationalized.     

Comparative Hydrophobic Core Dynamics Between Wild-Type Amyloid-β Fibrils,Glutamate-3 Truncation,and Serine-8 Phosphorylation

Post-translational modifications (PTMs) of amyloid-β (Aβ) species are implicated in the modulation of overall toxicities and aggregation propensities. We investigated the internal dynamics in the hydrophobic core of the truncated ΔE3 mutant fibrils of Aβ_1–40 and compared them with prior and new data for wild-type fibrils as well as with phosphorylated S8 fibrils. Deuteron static solid-state NMR techniques, spanning line-shape analysis, longitudinal relaxation, and chemical exchange saturation transfer methods, were employed to assess the rotameric jumps of several methyl-bearing and aromatic groups in the core of the fibrils. Taken together, the results indicate the rather significant influence of the PTMs on the hydrophobic core dynamics, which propagates far beyond the local site of the chemical modification. The phosphorylated S8 fibrils display an overall rigidifying of the core based on the higher activation barriers of motions than the wild-type fibrils, whereas the ΔE3 fibrils induce a broader variety of changes, some of which are thermodynamic in nature rather than the kinetic ones.     

Adiabatic Landau–Zener transitions at avoided level crossings with fast noise

Effects of a fast classical noise on adiabatic   Landau–Zener (LZ) transitions between the (2S+1)$(2S+1)$ Zeeman multiplets (diabatic states) of an arbitrary spin S$S$ at an avoided level crossing are investigated. The spin system is simultaneously coupled to a slow regular magnetic field and a fast   random field with Gaussian realizations. In the longitudinal direction, the magnetic field changes its sign at the degeneracy point (and is unbounded at large positive and negative times t=±∞$t=\pm \infty$ far from the degeneracy point) while in its single transverse direction, it remains of constant amplitude. The noise is considered in the limit where its characteristic correlation time (decay time) is small enough compared to the characteristic time of adiabatic LZ transitions. With these considerations, the condition for adiabatic evolution allows us to analytically evaluate the populations of diabatic levels and coherence factors. The study is first implemented for two- (S=1/2$S=1/2$) and three- (S=1$S=1$) state systems and finally extended to arbitrary S$S$. A numerical study is implemented allowing us to check/confirm the range of validity of our analytical solutions. We found a satisfactory quantitative agreement between numerical and analytical data.