全文获取类型
收费全文 | 4389篇 |
免费 | 439篇 |
国内免费 | 259篇 |
学科分类
医药卫生 | 5087篇 |
出版年
2024年 | 19篇 |
2023年 | 69篇 |
2022年 | 161篇 |
2021年 | 235篇 |
2020年 | 179篇 |
2019年 | 152篇 |
2018年 | 144篇 |
2017年 | 124篇 |
2016年 | 116篇 |
2015年 | 197篇 |
2014年 | 253篇 |
2013年 | 195篇 |
2012年 | 300篇 |
2011年 | 380篇 |
2010年 | 193篇 |
2009年 | 147篇 |
2008年 | 250篇 |
2007年 | 220篇 |
2006年 | 213篇 |
2005年 | 235篇 |
2004年 | 173篇 |
2003年 | 114篇 |
2002年 | 110篇 |
2001年 | 102篇 |
2000年 | 112篇 |
1999年 | 131篇 |
1998年 | 95篇 |
1997年 | 78篇 |
1996年 | 57篇 |
1995年 | 37篇 |
1994年 | 55篇 |
1993年 | 31篇 |
1992年 | 54篇 |
1991年 | 35篇 |
1990年 | 15篇 |
1989年 | 28篇 |
1988年 | 21篇 |
1987年 | 15篇 |
1986年 | 12篇 |
1985年 | 9篇 |
1984年 | 4篇 |
1983年 | 4篇 |
1982年 | 1篇 |
1981年 | 6篇 |
1980年 | 1篇 |
1979年 | 3篇 |
1969年 | 1篇 |
1928年 | 1篇 |
排序方式: 共有5087条查询结果,搜索用时 93 毫秒
61.
Haoyu Zhao Hongjiang Ruan Yuting Cao Hengfeng Yuan Qinglin Kang 《Orthopaedic Surgery》2022,14(12):3417
ObjectiveThe outcome of congenital clubfoot treatment is still challenging if the feet deformities are not completely corrected. Here we explore a minimal invasive procedure with an eight‐plate implant to correct the residual forefoot adduction deformity after treatment of neglected or relapsed clubfoot.MethodsWe retrospectively reviewed patients with residual forefoot adduction deformity after clubfoot treatment between January 2013 and June 2016. The patients underwent temporary epiphysiodesis of the lateral column of the mid‐foot, which in detail, an eight‐plate was placed on each side of the calcaneocuboid joint. The foot deformities were recorded according to the weight‐bearing radiographic measurements including talo‐first metatarsal angle, calcaneo‐fifth metatarsal angle and medial‐to‐lateral column length.ResultsA total of 13 patients (20 feet) with an average age of 7.8 years old were located with an average duration of 40.8 months follow‐up (range, 28 to 54 months). The average talo‐first metatarsal angle improved from 28.3° (range, 19° to 47°) preoperatively to 8.3° (range, 3° to 18°) and the calcaneo‐fifth metatarsal angle improved from 29.1° (range, 19° to 40°) preoperatively to 8.4° (range, 0° to 21°) at final follow‐up. The mean ratio of the medial‐to‐lateral column length improved from 1.14 ± 0.06 to 1.55 ± 0.09 with statistical significance (t = 3.566; P < 0.001).ConclusionsEight‐plate epiphysiodesis is an easy and effective method for the correction of residual forefoot adduction deformity after clubfoot treatment in growing children without the need of osteotomy. 相似文献
62.
目的:寻找抑制肺动脉高压肺血管重建的药物。方法:体外培养的肺动脉平滑肌细胞(PASMC)加入中药制剂黄芪口服液、前胡口服液、白蒺藜口服液分别大、小两个剂量(大剂量10mg/ml,小剂量1mg/ml)干预,并掺入~3H-胸腺嘧啶脱氧核糖核苷(`3H-TdR),~3H-脯氨酸(~3H-Pro)检测每分钟液闪值(cpm),反映 PASMC 增殖及胶原合成分泌的改变。结果:~3H-TdR掺入值在加入黄芪、前胡、白蒺藜口服液后,无论大、小剂量,PASMC 均被抑制(P<0.001);对~3H-pro 的掺入值,在加入黄芪、前胡口服液后,大剂量、小剂量皆有阻抑作用(P<0.01和 P<0.05),白蒺藜只在大剂量时有作用(P<0.01)。结论:中药制剂黄芪、前胡、白蒺藜口服液可不同程度地阻抑 PASMC 的增殖和胶原合成过程,可能对肺动脉高压肺血管结构的重建起一定防治作用。 相似文献
63.
鲨肝活性肽对对乙酰氨基酚致小鼠急性肝损伤的保护作用 总被引:7,自引:0,他引:7
目的探讨鲨肝活性肽(sHSS)对对乙酰氨基酚(AAP)致小鼠急性肝损伤的保护作用。方法用AAP(200 mg·kg-1,ip)诱导小鼠急性肝损伤,用改良赖氏法测血清ALT和AST,通过光镜和电镜观察肝细胞显微和亚显微结构的变化,用流式细胞仪分析肝细胞凋亡,同时用RT-PCR方法分析Fas mRNA表达水平。结果sHSS 3.0和1.5 mg·kg-1可显著降低肝损伤小鼠血清ALT和AST的水平;改善模型鼠肝组织细胞坏死及炎症反应;高剂量sHSS(3 mg·kg-1)对肝线粒体具有保护作用,下调Fas mRNA的表达水平,并具有抗凋亡作用。结论sHSS对AAP诱导的肝损伤具有明显的保护作用,其机制可能与保护肝线粒体、抑制Fas基因表达及肝细胞凋亡有关。 相似文献
64.
黄附子中性多糖和酸性蛋白多糖的分离、纯化与鉴定 总被引:10,自引:0,他引:10
目的 :探讨黄附子中的糖复合物 ,为深入研究和开发利用附子提供有用资料。方法 :采用热水抽提、乙醇沉淀、DEAE C32及SephadexG75柱层析等方法分离黄附子中的糖复合物 ,用酚 硫酸法、Folin 酚法及硫酸 咔唑法测定其糖、蛋白质、葡萄糖醛酸的含量。结果 :从黄附子中得到两种糖复合物 ,一种为中性多糖 ,含量为 3% ;另一种为酸性蛋白多糖 ,含量为 1% (其中糖含量 97%、蛋白质含量 3%、葡萄糖醛酸含量 9% )。结论 :黄附子中两种糖复合物的获得有助于研究附子抗癌、抗衰老和增强免疫机能的作用。 相似文献
65.
Major depressive disorder (MDD), also referred to as depression, is one of the most common psychiatric disorders with a high economic burden. The etiology of depression is still not clear, but it is generally believed that MDD is a multifactorial disease caused by the interaction of social, psychological, and biological aspects. Therefore, there is no exact pathological theory that can independently explain its pathogenesis, involving genetics, neurobiology, and neuroimaging. At present, there are many treatment measures for patients with depression, including drug therapy, psychotherapy, and neuromodulation technology. In recent years, great progress has been made in the development of new antidepressants, some of which have been applied in the clinic. This article mainly reviews the research progress, pathogenesis, and treatment of MDD. 相似文献
66.
对2型糖尿病机理的研究越来越受到人们的关注.本文就针刺对实验性2型糖尿病作用机理的进展情况从胰岛素抵抗、阿片肽、胰岛组织形态学、机体内环境四个方面进行了综述,并指出不足之处和提出了该领域的研究方向. 相似文献
67.
68.
69.
目的 分析桂枝茯苓胶囊联合西药治疗慢性盆腔炎患者疗效及对患者炎症因子的影响.方法 慢性盆腔炎患者70例随机分为研究组与对照组各35例.研究组采用桂枝茯苓胶囊联合西药治疗,对照组仅采用西药治疗.两组患者治疗疗程均为3个月经周期.结果 研究组治疗总有效率(94.29%)高于对照组(68.75%),差异有统计学意义(P<0.05).研究组患者治疗后血清IL-10[(27.62±4.67)μg/L]高于对照组[(18.42±3.46)μg/L],CRP[(5.42±1.27) mg/L]和TNF-α[(1.56±0.34) pg/ml]低于对照组[(8.13±1.76) mg/L,(2.65±0.54) pg/ml],差异有统计学意义(P<0.05).研究组不良反应发生率(5.71%)低于对照组(25.71%),差异有统计学意义(P<0.05).研究组随访6个月复发率(2.86%)低于对照组(34.29%),差异有统计学意义(P<0.05).结论 桂枝茯苓胶囊联合西药治疗慢性盆腔炎患者疗效显著,且能够增加血清IL-10含量和降低CRP、TNF-α含量,且未见严重用药不良反应、复发率低. 相似文献
70.
Hydrolyzates of silkworm pupae (Bombyx mori) protein is a new source of angiotensin I-converting enzyme inhibitory peptides (ACEIP) 总被引:2,自引:0,他引:2
Wang W Shen S Chen Q Tanga B He G Ruan H Das UN 《Current pharmaceutical biotechnology》2008,9(4):307-314
Silkworm pupae protein is a good source of high quality protein. The hydrolyzates of silkworm pupae protein catalyzed by neutrase, pepsin, acidic protease (Asperqiius usamii NO. 537), flavourzyme, alcalase, and trypsin with inhibitory activity on angiotensin I-converting enzyme (ACE) were identified by HPLC. The hydrolyzates catalyzed by acidic protease exerted the highest inhibitory activity on ACE. The hydrolyzing conditions were optimized by one-factor, factional factorial (FFD), and center composite (CCD) design methods, and response surface methodology (RSM). Statistical analyses showed that regression of the second-order model equation is suitable to describe ACE inhibitory bioactivity. The predicted inhibitory activity of hydrolyzates on ACE was 73.5 % at a concentration of 2.0 mg/ml. Optimized RSM technique decreased IC(50) of hydrolyzates inhibiting ACE to 1.4 mg/mL from 2.5 mg/ml. The molecular weight of the components of the hydrolyzates with inhibitory activity on ACE varied from less than 500 to about 1000 Da by ultra-filter analysis. These studies suggest that hydrolyzates of silkworm protein contain ACE inhibitory activity that could form a potential source of ACE inhibitor drugs. 相似文献