海口地区超广谱β-内酰胺酶阳性菌检测及其流行病学分析

目的为了解海口地区超广谱β-内酰胺酶(ESBLs)阳性菌的种类及其分布,为控制其扩散、预防暴发流行提供科学依据. 方法采用双纸片琼脂扩散法,测定3年中从临床各类标本分离的革兰阴性菌的ESBLs,比较菌种、病房及标本间ESBLs阳性率差异. 结果产ESBLs的菌种已达28种,阳性率最高达60%,常见菌的阳性率均达约50%,且3年间的变化多数无统计学意义;ESBLs阳性菌分离率在病室间的差异不大,门诊患者相对住院患者较低,在标本间的分布存在一定差异. 结论 ESBLs阳性菌已在海口地区广泛存在,并已成为医院及社区感染的主要病原菌,控制其进一步发展已成为一项迫在眉睫的工作.     

巧按穴位 预防感冒

春季是感冒的高发季节。在日常生活中，试着坚持按摩以下穴位，可以预防或减轻患感冒的概率。     

健康教育对腔隙性脑梗死患者生活方式的干预作用

目的:探讨危险意识性健康教育对腔隙性脑梗死患者生活方式的影响。方法:将116例腔隙性脑梗死患者随机分为研究组(n=58例)和对照组(n=58例),两组均给予相对统一的常规治疗、护理,在此基础上研究组进行危险意识性健康教育,对照组进行指示性健康教育,观察干预前后两组生活方式的转变情况。结果:研究组干预后生活方式较对照组有显著改变(P0.01)。结论:危险意识性健康教育使腔隙性脑梗死患者充分认识到生活方式对健康的重要性,建立并保持了健康的生活方式,有效控制了危险因素,是一种重要的辅助治疗措施,值得在临床上推广使用。     

顽固性心律失常采用胺碘酮静脉用药并口服治疗的效果探讨

目的对胺碘酮静脉用药并口服治疗顽固性心律失常的临床效果进行探讨。方法随机选择30例于2012年1月至2013年7月间在我院进行顽固性心律失常治疗的患者资料进行回顾性研究和分析,对全部患者进行胺碘酮静脉用药并口服治疗,观察和分析治疗效果。结果21例患者治疗显效,7例患者治疗有效,2例患者治疗无效,治疗总有效率为93%,1例患者血压下降,1例患者心慌气短,不良反应发生率为7%。结论对顽固性心律失常患者进行胺碘酮静脉用药并口服治疗能够取得理想的临床治疗效果,降低不良反应发生率,改善和优化患者的身体素质和生活质量。     

鼓浪屿综合疗养对高原脱适应官兵凝血功能的影响分析

目的探讨鼓浪屿疗养因子对高原脱适应官兵凝血功能的影响。方法试验组:高原人群分别于入院的第2、11天清晨空腹采血,检测凝血酶原时间(PT)、凝血酶时间(TT)、活化部分凝血活酶时间(APTT)、纤维蛋白原(FIB)。对照组:久居海滨人员于高原人群入院的第2天检查凝血功能。结果试验组高原官兵前后10 d的海滨疗养PT、TT、APTT、FIB比较差异无统计学意义(P0.05),试验组疗养前后与对照组比较差异有统计学意义(P0.05)。结论试验组高原脱适应官兵10 d海滨疗养对凝血功能无影响。     

抑制白血病K562细胞FoxM1表达可增强细胞对高三尖杉酯碱的敏感性

目的:探讨抑制白血病K562细胞叉头框蛋白M1(Fox M1)是否增强细胞对高三尖杉酯碱(HHT)的敏感性。方法:HHT以不同浓度(0、0.015、0.030和0.045μmol/L)和最低起效浓度不同时间(0.015μmol/L,0、24、48和72 h)作用于K562细胞,real-time PCR和Western blot检测Fox M1 mRNA和蛋白表达;以0.015μmol/L HHT作用K562细胞后转染Fox M1 siRNA,观察沉默K562细胞Fox M1后细胞对HHT的敏感性、细胞增殖和凋亡效应以及Fox M1相关靶分子c-Myc和Sp1表达状况。结果:随着HHT浓度增加和时间延长Fox M1表达逐渐降低,说明HHT抑制K562细胞Fox M1表达;HHT处理K562细胞后转染Fox M1 siRNA,细胞生长和克隆形成显著下降,细胞凋亡增加,因此抑制Fox M1可增加K562细胞对HHT的敏感性;Fox M1 siRNA组c-Myc和Sp1表达显著降低,表明Fox M1可正性调控c-Myc和Sp1表达。结论:HHT可以抑制白血病K562细胞Fox M1表达,干扰Fox M1可增强细胞对HHT的敏感性。     

Association of IL33/ST2 Signal Pathway Gene Polymorphisms with Myocardial Infarction in a Chinese Han Population

This study investigated the relationship between IL-33/ST2 signal pathway gene polymorphisms and myocardial infarction(MI) in Han Chinese. A case-control association analysis was performed on a total of 490 MI patients(MI group) and 929 normal subjects(NC group). Sequenom Mass Array and Taqman genotyping technique were used to analyze the tag single nucleotide polymorphisms(SNPs) in the genes encoding IL-33, ST2, and IL-1Ra P(rs11792633, rs1041973 and rs4624606). The results showed that the frequencies of rs4624606 genotypes AA, TT, AT were 0.031, 0.647, 0.322 in MI group and 0.026, 0.712, 0.263 in NC group, and the allele frequencies of A and T were 0.192, 0.808 in MI group and 0.157, 0.843 in NC group. There were significant differences in rs4624606 genotypes and allele frequencies between MI group and NC group(P<0.05). For rs11792633, the allele frequencies of C and T were 0.45, 0.55 in MI group and 0.454, 0.546 in NC group with no significant differences found between the two groups. Compared with genotype CC+TC, rs11792633 genotype TT had an increased risk of hypertension(P<0.05). However, there were no significant differences in the frequencies of rs11792633 genotypes between the two groups. No significant differences were noted in the frequencies of rs1041973 genotype and allele between the two groups. Logistic regression analysis showed that rs4624606 genotypes AT and AA+AT were both significantly associated with MI(AT: OR=1.325, P=0.029, 95% CI=1.03–1.705; AA+AT: OR=1.316, P=0.028, 95% CI=1.03–1.681) after factors such as age, gender, smoking, drinking, body mass index(BMI), triglyceride(TG) and cholesterol were adjusted. Those carrying rs4624606 genotype AT or AA+AT had an increased risk of MI. No associations were found between the polymorphisms of the other two loci with MI. It was concluded that, in the IL33/ST2 signal pathway, the A allele of rs4624606 polymorphism of IL-1Ra P gene is a potential independent risk factor for MI, and the genotypes AA+AT and AT are associated with the incidence of MI.     

BP网络在麻醉深度监测算法上的应用

麻醉是临床手术中必不可少的环节,但麻醉的过深或过浅可能给病人带来伤害,因而对麻醉深度的监测具有较高的临床价值。脑电是目前检测麻醉深度最有潜力的方法,首先通过滤波等处理方式得到较为纯净的脑电信号,分析时域和频域的特征,计算相应的参数,并将该参数作为前向反馈神经网络的输入参数,选择合适的BP神经网络拟合得到一个能够评价麻醉深度的无量纲常数。使用BP神经网络拟合结果来表征麻醉深度准确率普遍在90%以上,反映了BP神经网络在麻醉深度监测上具有较高的应用价值。