2450MHz微波照射小鼠睾丸对胚胎发育的影响

目的：观察微波局部照射小鼠睾丸后两个月与雌鼠交配对胚胎发育的影响。方法：雄性BALB/c小鼠随机分为1，2，3次照射组和对照组，应用2450MHz微波局部照射小鼠睾丸，待小鼠肛温升至（41&;#177;0．5）℃后维持15min，于雄鼠受照后两个月雌鼠交配，致雌鼠怀孕第18天脱颈处死孕鼠，辨认性别，活胎，死胎，吸收胎并对胎鼠称重，骨骼畸形采取茜素红染色，解剖显微镜观察。结果：照射组中吸收胎和死胎率均略高于对照组，平均每胎的植入数入数在各照射组均稍低于对照组，性别比各组均以雌性略高于雄性，仔鼠畸形的发生率有随着雄鼠受照射次数的增加而增加的趋势，但各指标在照射组和对照组间均无显著性差异（P＞0．05），结论：微波局部照射小鼠睾丸对生殖细胞有潜在的远后效应，一定程度上影响胚胎的发育，注意微波对生殖细胞的过量照射。     

The effects of integrin β1 antisense oligodeoxynucleotide on the expression of F-actin in ECV-304 cells exposed to infrasound

Objective To study the effect of integrin β1 antisense oligodeoxynucleotide(ASODN)on the expression of F-actin in ECV-304 ceils exposed to infrasound,and to explore the pathway of infrasound signal transfer into the cells.Methods integrin β1 oligodeoxynucleotides were embedded in cationic liposome lipofectamine 2000 reagent and transfected into ECV-304 cells.Laser scanning confoeal microscopv was used to observe F-actin's expression when the cells were exposed to 16 Hz infrasound at 130 dB for 2 h.The cells' average fluorescence was examined using spectrofluorimetric quantification after immunofluorescent staining. Results Among the three false exposure groups,most fluorescein-labelled substances in the cells were in a diffuse state.There were few actin filaments,and they were tenuous and short, without direction.The cells' fluorescence intensities were almost the same.In a group exposed to infrasound,F-actin was thick and long with a longitudinal arrangement.Fluorescence intensities were strong.However,less F-actin expression was observed in the ceils of the ASODN group exposed to infrasound compared with the others similarly exposed.Correspondingly.F-actin expression in the ASODN group exposed to infrasound was almost the same as in the other exposed group.Conclusion F-actin expression can be increased in ECV-304 cells by infrasound exposure,but integrin β1 ASODN may partially inhibit its expression.The extraeellular matrix-integrin-cytoskeleton may be one of the transduetion pathways for infrasound signals into the cells.     

The effects of integrin β1 antisense oligodeoxynucleotide on the expression of F-actin in ECV-304 cells exposed to infrasound

Objective To study the effect of integrin β1 antisense oligodeoxynucleotide(ASODN)on the expression of F-actin in ECV-304 ceils exposed to infrasound,and to explore the pathway of infrasound signal transfer into the cells.Methods integrin β1 oligodeoxynucleotides were embedded in cationic liposome lipofectamine 2000 reagent and transfected into ECV-304 cells.Laser scanning confoeal microscopv was used to observe F-actin's expression when the cells were exposed to 16 Hz infrasound at 130 dB for 2 h.The cells' average fluorescence was examined using spectrofluorimetric quantification after immunofluorescent staining. Results Among the three false exposure groups,most fluorescein-labelled substances in the cells were in a diffuse state.There were few actin filaments,and they were tenuous and short, without direction.The cells' fluorescence intensities were almost the same.In a group exposed to infrasound,F-actin was thick and long with a longitudinal arrangement.Fluorescence intensities were strong.However,less F-actin expression was observed in the ceils of the ASODN group exposed to infrasound compared with the others similarly exposed.Correspondingly.F-actin expression in the ASODN group exposed to infrasound was almost the same as in the other exposed group.Conclusion F-actin expression can be increased in ECV-304 cells by infrasound exposure,but integrin β1 ASODN may partially inhibit its expression.The extraeellular matrix-integrin-cytoskeleton may be one of the transduetion pathways for infrasound signals into the cells.     

电离辐射相关低密度寡核苷酸基因芯片的制备

目的：构建一种适用于辐射研究领域的低密度寡核苷酸基因芯片．方法：根据以往的研究报告,筛选参与辐射生物效应的基因和相关基因,从GenBank获取mRNA序列,使用Mprobe软件设计特异的探针,构建低密度辐射相关的基因芯片．在不同辐射敏感性肺癌细胞系中对该芯片的灵敏度和特异性进行评价,并对一些差异表达基因进行RT—PCR验证,以确认芯片检测结果的可信性．结果：构建出了辐射相关的寡核苷酸基因芯片,该芯片共含有143个基因,即127个辐射相关基因和16个对照基因．在不同辐射敏感性的A549和NCI—H446细胞中,筛选出18个差异表达基因．经RT—PCR对4个基因的验证,结果与芯片资料较一致．结论：辐射相关低密度寡核苷酸基因芯片可以检测不同辐射敏感性肿瘤细胞的差异表达基因,为辐射领域的研究提供了一种技术平台．     

电磁幅射致儿童淋巴瘤的机制及研究现状

当今社会电磁污染日益严重 ,越来越多的研究表明电磁幅射与儿童淋巴瘤的发病率具有相关性。本文就其流行病学和科学实验进行分析 ,从细胞水平、基因水平和信号传导方面综述了电磁场诱发儿童淋巴瘤的可能机制     

研究生微循环学教学改革初探

为了深化医学教育改革,全面推进素质教育,培养高层次医学人才,对硕士研究生微循环学教学,以强化素质教育为切入点,在教学内容、教学方法等方面进行了改革,取得了较好的教学效果。     

微波照射小鼠对微核的影响

微波辐射是否可引起遗传物质的改变，尚存在争议。应用２４５０ＭＨ２微波，分别一次全身照射ＢＡＬＢ／ｃ小鼠３００，６００，９００ｍＷ／ｃｍ＾２，以多核淋巴细胞检测法，观察了微波辐射对黄嘌呤－鸟嘌呤核糖转移酶基因的影响。结果表明，９００ｍＷ／ｃｍ＾２照射组和对照间出现非常显著的差异（Ｐ〈０．０１）。证明微波辐射对遗传物质的影响是明确存在的。     

大剂量~(60)Co照射对大鼠红细胞胰岛素受体的影响

受体是外界信息进入细胞的门户,是细胞生命活动过程中的始发点。受体正常功能的发挥,要求受体和激素都必须有正常的质和量,否则其生理功能就无法实现。在辐射对受体影响的研究中,多数是观察淋巴细胞受体,其主要反映辐射对免疫功能的影响,辐射对激素受体的影响尚未见报道。     

氮氧自由基R-1对人胃上皮细胞GES的辐射防护作用

目的探讨氮氧自由基R-1(下称R-1)对人胃上皮细胞GES电离辐射的防护作用。方法应用MTT法检测R-1对GES细胞24 h、48 h和72 h的毒性作用。给予0、1、2、4、8Gy的60Co-γ射线辐照后72 h和12 d分别采用MTT法和克隆形成实验,检测不同浓度R-1对GES细胞辐射防护作用。选取防护效果最佳的0.125μmol/L浓度组对GES细胞进行预处理,给予4Gy的60Co-γ射线照射后的24 h、48 h、72 h分别采用Hoechst33258荧光染色法和流式细胞仪观察GES细胞凋亡。结果与0μmol/L浓度组相比,当R-1的浓度低于1μmol/L时,GES细胞各时间点的吸光值无明显变化(P>0.05),而浓度高于2μmol/L时,其吸光值随浓度的增高下降(P<0.05)。选用0.1、0.125、0.25、0.5、1μmol/L的浓度组检测辐射防护作用,与0μmol/L组相比,R-1各浓度组的吸光值和克隆形成率提高(P<0.05)。故采用辐射防护效果最佳的0.125μmol/L浓度组处理后续实验,与照射组相比,药物+照射组的GES细胞数量明显增多、折光性强、轮廓清晰、微核和核碎裂数量减少、凋...