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BACKGROUND: Reperfusion injury salvage kinase (RISK) pathway plays an important role in protective mechanism against ischemia reperfusion injury (IRI) induced by both ischemic pre- and post-conditioning. Many researches have been carried out on RISK pathway mechanism underlying ischemic post-conditioning conferring cardioprotection against IRI; however, there is less research about its effect on IRI in the skeletal muscle.
OBJECTIVE: To investigate the protective effect of an optimized protocol of ischemic post-conditioning on IRI in rat skeletal muscle and its underlying mechanism.
METHODS: Eighteen male Sprague-Dawley rats were equivalently randomized into IRI, ischemic post-conditioning and control groups. Rats were given occlusion or disocclusion of the right femoral artery of the right lower limb. Subsequently, the IRI group rats were subjected to 24 hours of reperfusion; the ischemic post-conditioning group immediately given 4 cycles of 30 seconds reperfusion/30 seconds ischemia, followed by 24 hours of reperfusion; the control group given no intervention.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining showed that in the ischemic post-conditioning group, the morphology of muscle fibers changed little, with fewer inflammatory lesions and milder edema compared with the IRI group. The infarct size with TTC staining in the ischemic post-conditioning group was smaller than that in the IRI group. Western blot analysis revealed that the expressions of phospho-Akt and phosphorylated endothelial nitric oxide synthase-S1177 were significantly increased, but the expression of phosphorylated type endothelial nitric oxide synthase-Thr495 was much decreased in the ischemic post-conditioning group compared with the IRI group. The measurement of mitochondrial permeability transition pore opening with Ca2+ induction showed that the absorbance values in the ischemic post-conditioning group were significantly lower than those in the IRI group (P < 0.05). These results indicate that ischemia-reperfusion injury can be improved by applying an optimal protocol of ischemic post-conditioning in rat skeletal muscle. The underlying mechanism may be associated with the activation of RISK signaling pathway to inhibit opening of mitochondrial permeability transition pore, thereby contributing to the enhanced tolerance to IRI in rat skeletal muscle.
中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程 相似文献
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目的探讨甲状腺功能亢进症患者血清IL-17的水平及其FT3、FT4的相关性。方法采用酶联免疫吸附试验测定30例健康对照者和30例甲亢患者血清中IL-17的水平,化学发光法检测FF3、FT4,并分析IL-17与FT3、FT4的相关性。结果甲亢组血清中的IL-17(1089.5±101.9)pg/L与正常对照gg(725.4±94.6)pg/L比较明显升高(P〈0.05);同时相关性分析表明甲亢患者血清中IL-17的表达水平与FIB、FT4的浓度呈明显的正相关[r(FT3)=0.68,r(FT4)=0.56,P〈0.05]。结论甲亢患者血清中IL—17的高表达可能与FT3、FT4激素分泌有关。 相似文献
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目的 探讨半开放护理模式对精神发育迟滞患者的影响。方法 选取2019年6月—2020年6月住院的54例精神发育迟滞患者作为研究对象,采用住院患者观察量表和Barthel指数评定量表分析半开放式护理模式对患者生活自理能力、社会能力、社会兴趣、精神病表现、退缩、抑郁等情况的影响。结果 与干预前相比,运用半开放式护理模式1年后,患者激惹、迟缓、社会兴趣、个人整洁、抑郁、精神症状、总积极得分、总消极得分以及自我照顾得分的差异有统计学意义(P<0.05)。结论 半开放式护理模式能改善患者的退缩行为,促使患者情绪平稳,降低患者冲动行为的发生率,提升患者的生活自理能力。 相似文献
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目的探讨脊椎钉棒内固定系统治疗在胸腰椎骨折中的应用效果。方法对我院2007年1月至2011年7月收治的63例胸腰椎骨折患者行脊椎钉棒内固定系统治疗,分析其临床治疗效果。结果我院收治的63例胸腰椎骨折患者经过脊椎钉棒内固定系统治疗后,46例(73.02%)患者感觉运动肌力完全恢复,13例(20.63%)患者感觉运动肌力部分恢复,4例(6.35%)患者感觉运动肌力无明显改变。对所有患者进行6个月随访,优良率93.65%,患者复位良好,效果满意。结论脊椎钉棒内固定系统对治疗胸腰椎骨折,并对具有脊髓压迫症状的患者,具有良好的稳定脊椎的作用,患者康复情况良好,并发症少,临床治疗效果良好,具有重要的临床应用价值。 相似文献
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肝豆状核变性又称Wilson病(WD),是一种常染色体隐性遗传铜代谢障碍性疾病,致病基因(ATP7B基因)定位并克隆于13号染色体长臂染色体(13q14-q21)[1],ATP7B基因突变导致机体铜代谢异常,过量的铜沉积在肝脏和脑等组织中,引起不同程度的肝脏损伤和神经功能障碍。由于本病发病隐匿,侵及多组织器官,临床症状多样、不典型,不易早期诊断,易造成误诊、漏诊。现将我院2001年1月—2010年1月收治的45例WD患者的临床资料进行分析,以加深对该病的认识。 相似文献
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目的探讨有机磷中毒合并呼吸衰竭的危险因素。方法以226例口服有机磷中毒患者为研究对象,在中毒24 h内统计性别、年龄、口服量、开始洗胃时间、洗胃量、开始血液净化时间等20项指标。以有机磷中毒患者是否合并呼吸衰竭为因变量,上述20个观察指标中单因素分析差异有统计学意义的指标为自变量,采用二分类Logistic回归进行多因素分析。结果开始洗胃时间、消化道出血、尿隐血、肌酸激酶4个因素对有机磷中毒合并呼吸衰竭的发生有统计学意义。结论开始洗胃时间、消化道出血、尿隐血、肌酸激酶4个因素是有机磷中毒合并呼吸衰竭的独立危险因素。 相似文献