超声介导下重组腺相关病毒载体转染心肌的最佳滴度的实验研究

Objective To study the best titer of recombinant adeno-associated virus serotype 2 (rAAV2) delivered to myocardium by targeted ultrasound microbubbles. Methods Twenty one adult SD rats were divided into seven groups. SonoVue attached with different titer [1.5 × 109~ 3.0 × 1011 vg/ml (virus genome/ml)] of rAAV2-EGFP was infused into the tail vein of rats, following ultrasound mediated microbubbles destruction,as experiment groups. Normal saline was infused into the tail vein of rats as the control group (without rAAV2). Rats were killed after 14 days and hearts were harvested. GFP protein expression which showed rAAV2 transfer was observed under fluorescence microscope in frozen section and integrate optical density(IOD) was measured by Image Pro Plus software. Results When the titer of rAAV2 was 1.5 × 1011 vg/ml infused into the tail vein of rats there was much more GFP expression in myocardium than lower titers (P <0.01). Conclusions The best titer of rAAV2 delivered to myocardium by targeted ultrasound microbubbles is 1.5 × 1011 vg/ml in the tail vein infusion of rats.     

左心室下壁心内膜下囊肿超声表现1例

患者男,53岁。平时无特殊不适,常规体检,超声发现心内膜下囊肿1周入院。超声检查:各房室腔径正常,左室壁未见肥厚,左室下壁二尖瓣后叶瓣环下方距瓣环约 1.3 cm处心内膜下心肌层内可见 1.9 cm×2.8 cm的无回声区,无分隔,包膜完整,且回声较强(图1)。超声诊断:左室下壁心内膜下囊肿。CT检查未见明显异常。手术所见:心脏正常大小,左室后壁表面无特殊;探查左心腔,在二尖瓣后叶下方靠近瓣环约1 cm处,可见白色纤维组织膜,切开探查到一 2 cm×3 cm 大囊腔,向外靠近左室后壁仍有较厚的肌层,囊腔壁似纤维结缔组织。术后诊断:左室下壁心内膜下囊肿…     

叶酸干预对老年高血压患者血管内皮功能的影响

目的探讨叶酸对老年高血压患者血管内皮功能的影响。方法42例轻、中度老年高血压患者,随机分为叶酸干预组22例,对照组20例。采用高分辨率超声检测治疗前及治疗12周后肱动脉血流介导的血管内皮依赖性舒张功能与硝酸甘油介导的非内皮依赖性舒张功能。结果治疗前后两组间及组内肱动脉基础内径差异无统计学意义,叶酸干预治疗12周后与治疗前和对照组比较肱动脉血流介导的血管内皮依赖性舒张功能(11.59%±4.79%比7.15%±3.20%和8.14%±3.01%)、肱动脉硝酸甘油介导的非内皮依赖性舒张功能(19.73%±5.80%比16.69%±4.75%和17.55%±6.05%)均有明显改善(P<0.05)。结论叶酸对老年高血压患者血管内皮功能损害有一定的改善作用。     

急性心肌梗死延迟经皮冠状动脉成形术左室收缩功能的观察

目的观察急性心肌梗死(AMI)延迟PrCA术后左心室收缩功能. 方法25例AMI溶栓成功的患者观察1个月内及满6个月时左心舒张末期内径(LVED)和射血分数(MVEF),同时对25例AMI溶栓成功后延迟PTCA术(2～4周内手术获得TIMI血流Ⅲ级者)观察同样指标,二者进行比较.     

超声诊断急性特发性阴囊水肿1例

<正>患儿男,1岁,因左侧阴囊昆虫叮咬后红肿、疼痛1 d来我院就诊。超声首次检查考虑左侧腹股沟疝,临床予以否认,故再次复查。检查过程中明确患儿有昆虫叮咬病史且阴囊肿痛程度较前明显好转,超声表现:右侧睾丸大小1.6 cm×0.8 cm,左侧睾丸大小1.6 cm×0.8 cm;双侧睾丸回声均匀,彩色血流未见异常。双侧附睾头、体、尾部均未肿大。左侧阴囊皮肤厚约1.0 cm(图1),右侧阴囊皮肤正常(图2),左侧阴囊皮肤增厚呈蜂窝状,内部血     

巨噬细胞极化的转录调控及其对相关疾病影响的研究进展

巨噬细胞作为固有免疫和适应性免疫的重要组成成分,在全身的新陈代谢、血管形成、细胞凋亡和抗肿瘤等方面具有重要的调节作用,这与其可塑性和异质性有关联。当受到环境刺激时,巨噬细胞可分化成具有不同功能的表型(即极化)。由于巨噬细胞极化对相关疾病的发生发展具有重要影响,调控巨噬细胞极化可为一些临床疾病的治疗提供方法,因此本文就巨噬细胞极化的转录调控及其与相关疾病的关系进行综述。     

心肌梗死的超声应变率显像研究

目的　探讨梗死心肌超声应变率的变化特点。方法　 2 0例陈旧性前壁心肌梗死患者 (OMI)与 2 0例正常对照者 (Nor) ,获取左室前壁长轴方向的心肌应变率曲线 ,分别测定收缩期、舒张早期和舒张晚期的峰值应变率 (SR)。结果　 (1) OMI组梗死节段心肌的 SR低于正常组相应节段的 SR (P<0 .0 5 )。 (2 )与相邻非梗死节段心肌相比 ,梗死节段心肌的 SR降低 (P<0 .0 5 )。结论　梗死心肌的 SR降低 ,与相邻正常心肌相比 SR的显著下降 ,是梗死心肌超声应变率的特征性改变。     

斑点追踪显像对心尖肥厚型心肌病左室功能的评价分析

目的 探讨斑点追踪显像评价心尖肥厚型心肌病左室收缩功能的改变.方法 记录18例心尖肥厚型心肌病患者(HCM组)和20例健康对照者(对照组),获取二维灰阶图像,分析各个心肌节段收缩期峰值的纵向应变(SL)、径向应变(SR)和周向应变(SC).结果 HCM组心肌整体SL、SR及SC收缩期峰值应变均明显低于对照组(P＜0.05).HCM组所有节段的收缩期峰值SL均明显低于对照组;与对照组相比,左房室瓣及乳头肌水平大多数节段的SR降低,左房室瓣及乳头肌水平个别节段的SC降低,心尖水平所有节段的SC及SR均明显降低(P＜0.05).结论 HCM患者左室长轴和短轴收缩功能均降低,以长轴功能减弱明显,局部心肌SL、SR及SC的变化可反映心肌的功能改变.     

超声介导下重组腺相关病毒载体转染心肌的最佳滴度的实验研究

Objective To study the best titer of recombinant adeno-associated virus serotype 2 (rAAV2) delivered to myocardium by targeted ultrasound microbubbles. Methods Twenty one adult SD rats were divided into seven groups. SonoVue attached with different titer [1.5 × 109~ 3.0 × 1011 vg/ml (virus genome/ml)] of rAAV2-EGFP was infused into the tail vein of rats, following ultrasound mediated microbubbles destruction,as experiment groups. Normal saline was infused into the tail vein of rats as the control group (without rAAV2). Rats were killed after 14 days and hearts were harvested. GFP protein expression which showed rAAV2 transfer was observed under fluorescence microscope in frozen section and integrate optical density(IOD) was measured by Image Pro Plus software. Results When the titer of rAAV2 was 1.5 × 1011 vg/ml infused into the tail vein of rats there was much more GFP expression in myocardium than lower titers (P <0.01). Conclusions The best titer of rAAV2 delivered to myocardium by targeted ultrasound microbubbles is 1.5 × 1011 vg/ml in the tail vein infusion of rats.     

轻度二尖瓣反流二尖瓣瓣叶和瓣环形态与功能变化的三维超声研究

目的 探讨经食管实时三维超声心动图(real time three dimensional transesophageal echocardiography,RT 3D-TEE)测量轻度二尖瓣反流(mitral regurgitation,MR)患者二尖瓣瓣叶和瓣环参数在二尖瓣反流机制中的作用.方法 对57例MR患者和28例无二尖瓣反流的对照组,分别行RT-3D-TEE检查,应用MVQ(mitral valve quantification)软件进行后处理,测量收缩末期前后叶闭合时瓣环参数:高度与联合直径比(H/DAIPm)、椭圆度(E2D)、主动脉瓣环与二尖瓣环平面夹角(θAv-Mv)、瓣环非平面角度(瓣环θnpa);瓣叶参数:小叶穹隆面积(A3DE)、投影平面中的对合长度( L2DAIPm)、瓣叶A1-A3区穹隆体积(VA1-3tent)、瓣叶P1-P3区穹隆体积(VP1-3tent)、瓣叶穹隆容积(Vtent)、瓣叶穹隆高度(Htent)、瓣叶非平面角度(瓣叶θnpa).比较两组结果,筛检出能够揭示MR机制的参数.结果 MR组:瓣环θnpa和L2DAIPm均较对照组增大,VA3tent较对照组减小,瓣叶θnpa与MR独立相关.结论 轻度二尖瓣反流组瓣环几何形态趋于平面化,瓣叶局部向瓣环方向的运动增强,瓣叶θnpa与轻度二尖瓣反流独立相关.