Inhibitory effects and mechanism of rhBMP-2 on the proliferation of SHG44 human glioma cells

Bone morphogenetic protein (BMP) has been denominated by its artivity of inducing bone formation. Re-cently, it was found to be a multifunctional factor andhave a key role in the regulation of elnbmpis andembryo deVelopment, hssue and cell ~ation andPndiferation. BMP could also inhibit many kinds of tumorcell growth and induce their apoptOSisL',']. the of BMPgenes were successop cloned and ~ssed in aam,such as BMP-2, BMP4 and BMP-7. The bone ~C Pmtein-2 is a htllnan P~ exPansed in vi…     

An experimental study on the expression of SV40 large tumor antigen in human brain tumors

In recent years, it has become a novel hot spotof oncology that the research on the relationship between simian virus 40 (SV40) and the etiology ofhuman brain tumors as well as other types of tumors.However, the presence of SV40 and the expression ofoncoprotein large tumor antigen (Tag) in human braintumors remain a controversial issue. It is known thathuman cells are only semipermissive for SV40 infection, even if SV40 is in human brain tumors indeed,whether SV40 is associated with human …     

代谢型谷氨酸受体在脑损伤后的表达及意义

目的：探讨弥漫性脑损伤（DBI）后大脑皮质代谢性谷氨酸受体（mGluRs)的变化及其意义。方法：SD大鼠随机分为对照组和DBI组,采用Marmarou加速性DBI模型,在伤后不同时间取样行原位杂交和病理学研究。结果：与对照组相比,DBI组大脑皮质I、Ⅲ组mGluRs(除mGluR6外）阳性神经元表达在伤后12小时开始增加,24小时增加显著（P＜0．01）。病理研究提示,DBI后24小时大脑皮质神经元损伤严重（P＜0．01）,与mGluRs表达变化的时间吻合。结论：I、Ⅲ组mGluRs作用不同,分别具有神经元损害和保护作用;脑损伤后,I组mGluRs表达增加,参与DBI引起的神经元损伤,Ⅲ组mGluRs表达增加,具有神经元保护作用,这为阐明DBI的损伤机制及运用mGluRs激动剂和（或）拮抗剂治疗提供了理论依据。     

抑癌基因PTEN诱导人脑胶质瘤SHG—44细胞凋亡

目的 研究外源性PTEN基因对人脑胶质瘤细胞系SHG－44凋亡的影响,探讨PTEN基因抑制肿瘤细胞增殖的机制。方法 以携有人PTEN基因的真核表达载体体外转染SHG－44细胞,筛选阳性转染的细胞克隆并扩增培养。以原位杂交和免疫组化染色法检测PTEN基因的表达。采用透射电镜、流式细胞仪和核DNA琼脂糖凝胶电泳,检测转染PTEN基因后SHG－44胶质细胞的凋亡。观察导入入PTEN基因对SHG－44细胞裸鼠致瘤能力的影响。结果 获得PTEN基因稳定转染的胶质瘤细胞SHG－44,并检测到PTEN基因和蛋白的表达。透射电镜下可见转染PTEN基因后细胞核染色质浓缩边集、胞浆浓缩、核碎裂及凋亡小体形成等典型的凋亡表面。流式细胞仪显示,细胞周期从G1期到S期发生抑制,并在G1期峰前出现1个明显的凋亡峰（12．9％）。细胞核DNA琼脂糖凝胶电泳显示凋亡细胞特朋的梯状条带。转染空载体及未转染的SHG－44细胞未见的凋亡表现。转染PTEN基因后,SHG－44细胞对裸鼠瘤能力明显降低。结论 将外源必PTEN基因导入SHG－44肿瘤细胞后,可诱导细胞凋亡,这可能是PTEN基因抑制肿瘤细胞增殖的重要机制之一。     

绿色荧光蛋白转染胶质瘤细胞的体内实验研究

 目的　利用体外转染绿色荧光蛋白 (Enhanced Green Fluorescent Protein,EGFP)基因的胶质瘤细胞建立大鼠移植模型 ,并探索利用该模型研究肿瘤体内侵袭的可行性。方法　携有EGFP基因的 p EGFP- N3质粒体外转染 C6胶质瘤细胞 ,筛选稳定表达 GFP的细胞克隆并以立体定向法植入 SD大鼠脑实质内建立肿瘤移植模型。 4周后处死大鼠并制作连续石蜡切片 ,相邻切片分别作 HE、免疫组化染色和荧光显微镜检测。结果　GFP于体内稳定表达 ,在荧光显微镜下可较容易区分肿瘤细胞和非肿瘤细胞 ,并能检测到侵袭至远端的单个肿瘤细胞 ,其检测效率明显高于HE和免疫组化染色。结论　EGFP基因体外转染 C6胶质瘤细胞并建立大鼠脑内肿瘤移植模型 ,是研究胶质瘤体内侵袭的有效模型和方法。     

SIGNIFICANCE OF THE EXPRESSION OF GREEN FLUORESCENT PROTEIN ON DETECTION OF GLIOMA INVASION IN VIVO

The study on biological character of glioma is rapidly expanding day by day. However, there are some shortcomings in present experimental model and examination methods in vivo, which could not easily permit the visualization of tumor invasion, especially for the single invasive tumor cell. Identification of small numbers or individual invading tumor cells against background of host CNS cells may be impossible by standard HE staining. The deficiency of specific antibody against glioma cell de…     

人脑胶质瘤组织内微血管定量的临床意义

目的．;探讨人脑胶质细胞瘤组织内微血管定量（ＭＶＱ）的临床意义。方法：应用免疫组织化学技术在６３例人脑胶质瘤组织中对第Ⅷ因子相关抗原进行表达,并计数肿瘤ＭＶＱ。结果：Ⅲ、Ⅳ级胶质瘤ＭＶＱ显著高于Ⅰ、Ⅱ级;术后１８个月内复发和生存期〈３年者ＭＶＱ分别 术后１８个月无复发及生存期〉３年者;ＭＶＱ与患者性别、年龄、３啼生长部位无明显相关。结论;人脑胶质瘤ＭＶＱ与病理分级、术后复发及生存期关系密切,可作为     

血管抑素K(1～3)基因片段的克隆与序列测定

我们用自己设计的一对引物,应用ＰＣＲ法成功获得血管抑素(ａｎｇｉｏｓｔａｔｉｎ)Ｋ(1～3)基因片段,为进一步研究其表达和实体瘤抗血管生成治疗创造条件,现将结果报道如下。一、材料和方法1.菌种、载体及模板:大肠杆菌Ｅ.ＣｏｌｉＤＨ5α,基因型ｓｕｐＥ44,ｈｓｄＲ17ｒｅｃＡｌｅｎｄＡｌｇｙｒＡ96ｔｈｉ1ｒｅｌＡ1,载体ｐＧＥＭ3Ｚｆ( )为本室保存;人白细胞ｃＤＮＡ文库购自美国Ｃｌｏｎｔｅｃｈ公司。2.引物设计　克隆血管抑素Ｋ(1～3)Ｎ端功能区的引物如下,在其末端引入ＥｃｏＲＩ和ＢａｍＨＩ酶切位点,引物由上海生物工程公司合成。…     

Cloning and sequencing of the fragment of angiostatin K(13) gene

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