Sub-nanomolar detection of thrombin activity on a microfluidic chip

Bioluminescence resonance energy transfer (BRET) is a form of Förster resonance energy transfer. BRET has been shown to support lower limits of detection than fluorescence resonance energy transfer (FRET) but, unlike FRET, has not been widely implemented on microfluidic devices for bioanalytical sensing. We recently reported a microscope-based microfluidic system for BRET-based biosensing, using a hybrid, high quantum-efficiency, form of BRET chemistry. This paper reports the first optical fiber-based system for BRET detection on a microfluidic chip, capable of quantifying photon emissions from the low quantum-efficiency BRET² system. We investigated the effects of varying core diameter and numerical aperture of optical fibers, as well as varying microfluidic channel design and measurement conditions. We optimized the set-up in order to maximize photon counts and minimize the response time. The optimized conditions supported measurement of thrombin activity, with a limit of detection of 20 pM, which is lower than the microscope-based system and more than 20 times lower than concentrations reported to occur in plasma clots.     

Developmental dyslexia,cerebellar/vestibular brain function and possible links to exercise-based interventions: a review

The Phonological Processing Deficit (PPD) hypothesis remains the most influential theory to explain why some children fail to acquire appropriate reading skills. However, current research suggests that there may be other deficits operating, and that the phonological processing deficit may be just one manifestation of a deeper underlying anatomical syndrome that originates in the cerebellar or vestibular areas of the brain. Claims that exercise regimes or programmes of vestibular stimulation may provide a ‘cure’ for developmental dyslexia (specific reading difficulties) prompt scepticism among the scientific community and raise concerns about the exploitation of vulnerable parents. The paper provides a review of research into the causes of specific reading difficulties at the behavioural, cognitive and biological level of explanation, and considers whether or not there is any theoretical basis for the use of exercise-based intervention programmes. Following consideration of recent findings from a range of scientific disciplines, it is concluded that such ‘unconventional’ methods of intervention may draw some theoretical support from the scientific literature, although researchers are cautioned to be wary of comorbidity issues. It is also concluded that more rigorous and independent evaluations of the success (or otherwise) of exercise-based interventions are needed.     

Conceptualizing curriculum management in an effective secondary school: a Western Australian case study

ABSTRACT

This paper investigates the linkages between management processes and the curriculum using an academically effective secondary school as a case study. The aims of the study are twofold. First, in view of the acknowledged importance governments and others place on the quality of school leadership and management on the one hand and curriculum, teaching and learning reforms on the other, there is a need for empirical data as to how school leaders and managers work to secure improvements in curriculum, teaching and learning. This study purports to provide such data. The second aim acknowledges the need to develop conceptual frameworks and data collection instruments as aids to better understanding of complex relationships within school organizations.two deputy principals do play an indirect and important part in promoting academic performance by reinforcing school values in a wide variety of ways. Third, curriculum management is the responsibility of senior teachers and there is tight linkage between both senior teachers and teachers and high quality teaching and learning. Finally, the loose linkage between departments, and also between departments and senior management, provides little opportunity for a school‐wide approach to curriculum matters. It is evident that a whole‐school curriculum management perspective is absent in this school.     

“Forget birth pains,we haven't got past the conception stage yet”. A reply to Frank Coffield's ‘Britain's continuing failure to train: the birth pangs of a new policy’

This article is a response to Frank Coffield's recent reflections in this journal on the Performance and Innovation Unit's project on workforce development in Britain. It questions the extent to which the PIU's findings represent a ‘new understanding’ of the UK's skills problem and asks why academics have been so reluctant to spell out what a demand‐side strategy for tackling the problem would involve.