排序方式: 共有61条查询结果,搜索用时 167 毫秒
21.
为使微流控荧光PCR扩增循环的实际流速与理论设计流速吻合,保证芯片的PCR扩增过程与步骤的准确完成,必需对微通道中的实际工作流体流速情况进行实时测量和流速调控.利用在39个循环微通道的生物PCR微流控芯片中,充满强阳性荧光PCR试剂微通道的荧光信号值为空微通道的荧光信号值的4倍左右,为充满阴性荧光PCR试剂微通道荧光信号值的10倍左右的特性,在芯片上建立荧光实时测速信息反馈系统,对微通道中的实际工作流体流速情况进行实时反馈和控制. 相似文献
22.
23.
24.
25.
To study the stereostructure by X-ray and the technology of extracting acankoreanogenin from the leaves of Acanthopanax graeilistylus W. W. Smith (AGS), the crystal structure was measured with a Bruker APEX-Ⅱ area-detector diffractometer instrument and the technology of extracting in combination hydrolysis in situ (ECHS) was compared with these of traditional methods. The crystal belongs to the monoclinic system, space group P2b with unit cell parameters: a=(8.3652±0.0006) nm, b=(24.721±0.002) nm, and c=(14.5587±0.0011) nm, α=90°, β=97.850 (4) °, γ=90 °, V=2982.51 nm3, Dc= 1.179 mg/m3, and the molecular number (Z) of elementary structures was 2. The comparisons show that the extraction rate of acankoreanogenin with ECHS methods is much higher than that of traditional methods. Then, central composite design-response surface methodology (CCD-RSM) was adopted for optimizing the extraction rate of ECHS methods. The optimized values of extraction parameters are as follows: for the for extraction process of acid hydrolysis are that extraction time 110.8 min, solvent-herb ratio 11.5 and acid content 5.25%; the best extraction process of basic hydrolysis are that extract time 120 min, solvent-herb ratio 8.7 and the alkali content 8.79%. Finally, the extracts were purified with decolorizing carbon after alkali solution and acid-isolation and purity of acankoreanogenin was 98.7%. 相似文献
27.
28.
农村水环境污染产生原因及防治对策 总被引:1,自引:0,他引:1
建设社会主义新农村是我国现代化进程中的重大任务,而农村水环境污染问题已经成为制约新农村建设前进的重要因素。针对目前农村水环境污染问题产生的原因及其危害.以及水污染防治工作中存在的问题,提出了对策和建议,为农村水污染处理提供参考。 相似文献
29.
30.