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151.
152.
本文建立了在常温常速离心条件下进行样品预处理的人血浆三尖杉酯碱高效液相色谱荧光测定法。本法批内CV%为3.08~3.47%,批间CV%为4.36~5.28%,回收率范围为93.6~95%,标准曲线在12.5~500ng/ml范围内线性良好(r=0.998)。方法简单、快速、准确,适于临床应用。 相似文献
153.
An assessment of immunoreactive epidermal growth factor in urine of patients with urological diseases 总被引:1,自引:0,他引:1
N. H. Chow T. -S. Tzai P. -E. Cheng C. -J. Chang J. S. -N. Lin M. -J. Tang 《Urological research》1994,22(4):221-225
To examine the excretion of urinary epidermal growth factor (EGF) in urological diseases and the relationship of EGF urine levels with transitional cell carcinoma (TCC), we measured the concentration of EGF by radioimmunoassay. The series comprised patients with active TCC (n=50), others in tumor-free status (n=29) and with non-neoplastic inflammatory diseases (n=43), and normal controls (n=50). Urinary EGF values were lower in patients with urological diseases of different etiologies than in normal controls (P<0.005). Mean EGF levels of patients who had previous bladder tumor resection (n=21) were not statistically different from normal controls (P=0.2). For patients with active TCC, EGF urine levels showed a significant inverse relationship to increasing tumor grade (P=0.02). In addition, subjects who had received nephrectomy for pelvic carcinoma (n=8) showed significantly lower mean EGF values than those with intact kidneys (n=21), irrespective of sex (P<0.05). Immunostaining of EGF on non-neoplastic kidney (n=9) revealed reactivity in the distal convoluted tubules and thick ascending limbs of Henle. Our results suggest that the kidney is the major source of urinary EGF. Its excretion in urine is decreased in both inflammatory and neoplastic diseases of the urinary tract. EGF may play an important part in the biological activity of TCC. Further study is indicated to investigate the monitoring of EGF urine levels as a marker of recurrence for EGF receptor-positive TCC. 相似文献
154.
Jian-ya Zhou Fa-di Tang Guo-gen Mao Jie Shao Yan Wang Ru-lian Bian 《浙江大学学报(医学版)》2003,32(4):315-8, 326
OBJECTIVE: To study the effect of eucalyptus globulus oil on the activity of nuclear factor-kappaB(NF-kappaB) in THP-1 cell line. METHODS: THP-1 cells were cultured with or without eucalyptus globulus oil at different concentrations (1, 10, 100 mg x L(-1), 30 min) before being stimulated with lipopolysaccharide (LPS, 1 mg x L(-1), 30 min). The location of NF-kappaB p65 subunit (NF-kappaB/p65) in THP-1 cells was detected by indirect immunofluorescence and laser scanning confocal microscope. The expression of NF-kappaB/p65 in nuclei was measured by Western-blot analysis. RESULT: The FITC-label NF-kappaB/p65 was mainly located in the nuclei after THP-1 cells were stimulated with LPS. Whereas, no fluorescence were seen in the nuclei of cells pretreated with eucalyptus globulus oil. This effect on NF-kappaB/p65 nuclear translocation was in a concentration dependent manner. CONCLUSION: Eucalyptus globulus oil inhibits the nuclear translocation of NF-kappaB induced by LPS in THP-1 cells. 相似文献
155.
Xin-ping Tang Kang Zeng Guan-hua Chen Long-yan Bi Long-zhong Fan Chang-fa Shao 《第一军医大学学报》2003,23(8):870-872
OBJECTIVE: To investigate the association of Malassezia furfur with chronic urticaria in the crew members of ships. METHODS: A comparative mycological study of 126 crew members of ships with chronic urticaria and 45 normal control subjects was carried out. The 82 urticaria patients identified as positive for Malassezia furfur were divided into groups A and B to receive treatment with antihistaminics (group A) and antihistaminics combined with 2% ketoconazole shampoo(group A). RESULTS: The carrier rates of Malassezia furfur were significantly higher in urticaria patients than in the normal control subjects (P<0.01), but in view of the case ratios of the final cure or improvement, no significant difference was observed between the two groups by the end of the treatment courses (P>0.05). But 6 to 8 weeks from the end of the treatment course, better therapeutic effect was noted in group B (P<0.01), with higher rate of negative Malassezia furfur findings (P<0.01). CONCLUSION: Malassezia furfur may play an important role in the prevalence of chronic urticaria among the crew members, and anti-fungal treatment may produce better long-term therapeutic effect. 相似文献
156.
目的 探讨异位甲状腺癌的发病特点、诊断和治疗方法。方法 对 2 9例异位甲状腺癌的临床及病理资料进行回顾性分析。结果 2 9例均行手术治疗 ,术后辅以放、化疗。术后随访 ,5年存活率为 5 2 .0 % ,最长 1例已存活 2 4年。结论 早期发现并及时手术治疗是提高异位甲状腺癌患者生存率的关键 ,术后给予辅助性放、化疗有助于提高生存率。 相似文献
157.
高渗盐/甘露醇液对内毒素小鼠脑腺细胞凋亡的保护作用 总被引:2,自引:0,他引:2
为探讨高渗盐/甘露醇法(HSM)对内毒素诱导小鼠胸腺细胞凋亡的影响,取昆明纯种小白鼠24只平分为三组,即(1)对照组,腹腔注射生理盐水;(2)内毒素实验组(LPS组),腹腔注入内毒素;(3)LPS+HSM组,腹腔注入内毒素前后共注HSM二次。结果示,第三组胸腺细胞凋亡百分率及DNA断裂百分率明显低于第二组(P〈0.01),DNA断裂碎片中的琼脂糖凝胶电泳未见典型的梯状带。提示HSM对内毒素诱导的小 相似文献
158.
肾上腺髓质素对内皮素促家兔气道平滑肌细胞增殖的抑制作用 总被引:3,自引:0,他引:3
在体外培养的家兔气道平滑肌细胞(ASMC)上,观察肾上腺髓质素(AM)对内皮素(ET)促ASMC增殖的影响及丝裂素活化蛋白激酶(MAPK)活性的变化。以探讨AM对ASMC增殖的调控。结果显示10-8mol/LET-1显著刺激ASMC3H-TdR参入及MAPK激活(P<0.01)。AM(13-52)呈剂量依赖地抑制ET-1的上述作用(P<0.05,P<0.01)。单独应用AM(13-52)对ASMC3H-TdR参入及MAPK活性无明显影响。表明AM(13-52)可抑制ASMC对ET-1的增殖反应,其机理可能涉及MAPK活性的抑制。 相似文献
159.
目的:研究不同种类和强度基牙材料与瓷冠破折碎裂的内在关系.方法:选用计算机建立上颌中切牙的三维有限元模型,模拟陶瓷层、牙本质、核桩等结构.分析在天然齿基牙、钴铬合金基牙及复合树脂基牙情况下,铸造玻璃陶瓷全冠的瓷层内及残余牙本质的应力分布情况.结果:高弹性模量的钴铬合金能够降低瓷层及残余牙本质内的最大主应力和最小主应力峰值,防止瓷层破折碎裂;而弹性模量与牙本质相近的复合树脂桩降低应力峰值的作用不明显.结论:对于有牙髓活力的牙本质基牙,尽量保存牙髓活力后行全冠修复.对于行根管治疗术后的无髓牙,进行核桩冠修复时,采用高弹性模量的高熔合金,尽量避免使用弹性模量较小的复合树脂核. 相似文献
160.