Reduced expression of CD69 and adhesion molecules of T lymphocytes in asthmatic children receiving immunotherapy

T lymphocytes play a fundamental role in the initiation and regulation of chronic inflammatory responses in patients with asthma. CD69 is an early marker of T‐cell activation. The levels of intercellular adhesion molecule‐1 (ICAM‐1, CD54) and L ‐selectin have been reported to increase in patients with allergic diseases and asthma. The present study was therefore undertaken to investigate the expression of CD69, CD54, and L ‐selectin by T lymphocytes of children with asthma, before and after immunotherapy. Eighteen children newly diagnosed with asthma, 11 good and nine poor responders to immunotherapy, and 16 normal subjects, were enrolled in this study. The percentages of CD69⁺, CD54⁺, and CD62L⁺ cells in T lymphocytes were measured by using flow cytometry. The levels of CD69, CD54, and CD62L in serum and culture supernatants were determined by using enzyme‐linked immunosorbent assay (ELISA). The expression of CD69 and CD54 on CD3⁺ T lymphocytes was significantly higher in children with asthma than in control patients. All the patient groups expressed (spontaneously and following stimulation with phorbol myristate acetate and ionomycin together with mite‐extract proteins) greater amounts of CD69 and CD54 than did control subjects. With long‐term immunotherapy, the percentages of CD69⁺ and CD54⁺ T lymphocytes were significantly lower in patients with a good response to immunotherapy. Our results also showed significantly lower serum L ‐selectin levels following immunotherapy. In conclusion, successful immunotherapy resulted in decreased expression and production of CD69 and CD54. These results may explain, in part, the clinical efficacy of immunotherapy.     

脑创伤致迟发性脑梗死临床分析

目的探讨脑创伤后迟发脑梗死的发生机制,临床诊断及救治措施。方法对32例经影像学证实为颅脑创伤后迟发脑梗死患者的临床资料进行回顾分析。结果出院时按GOS标准评价:恢复良好12例、中残5例、重残4例、植物生存3例,死亡8例,其中非手术治疗20例,存活14例,死亡6例,死亡率30%;手术治疗12例,存活10例,死亡2例,死亡率17%。结论及时诊断与合理有效的治疗是提高脑创伤后迟发性脑梗死的治愈率及提高患者生存质量的关键。     

某部性病基本知识调查分析

目的　探讨部队性病防治情况。方法　设计 10个与性病有关的问题 ,对随机抽样某师 4个营官兵现场无记名调查。结果　有关性病的基本知识已被大部分官兵所认知 ,但也有一部分官兵对性病缺乏了解 ,甚至误解。结论　在官兵中加强性病基本知识的宣传 ,强调性病的危害对部队防治性病很有必要。     

安徽省农村居民哮喘病现况调查

本文通过对安徽省农村居民哮喘病的现况调查发现，哮喘病患病率为１．３３％，男性为１．４８％，女性为１．１９％；成人患病率为１．３４％，儿童为１．３２％。儿童哮喘患病率随年龄增长而下降，而成人哮喘病患病率随年龄增长而增加；儿童大部分婴幼儿起病，各年龄组中男性起病较女性晚，成人女性５０％起病２０岁前，而男性５０％起病３０岁前。农村哮喘以感染型为主，发病以冬季多见，发作主要诱因是感冒，哮喘病具有明显家族聚集性。     

Effect of Captopril on Antithrombus Function of Endothelium

ＥｆｆｅｃｔｏｆＣａｐｔｏｐｒｉｌｏｎＡｎｔｉｔｈｒｏｍｂｕｓＦｕｎｃｔｉｏｎｏｆＥｎｄｏｔｈｅｌｉｕｍＸＩＯＮＧＹｉ－ｌｉ（熊一力）；ＺＨＡＯＨｕａ－ｙｕｅ（赵华月）（ＤｅｐａｒｔｍｅｎｔｏｆＭｅｄｉｃｉｎｅ，ＴｏｎｇｊｉＨｏｓｐｉｔａｌ，Ｔｏｎｇ...     

等密度硬膜下血肿的CT诊断(附47例分析)

本文分析了47例等密度硬膜下血肿的CT表现,观测的各种CT征象,发现(1)bolus增强扫描显示的血肿、血肿膜及表面移位血管;(2)中线结构的特征性偏移;(3)表面脑回聚拢内移;(4)脑灰白质界面内移;(5)侧脑室变形和脑—脑室指数变小,对等密度硬膜下血肿的诊断和鉴别诊断有重要意义。     

人晶体 γ-晶体蛋白的研究:Ⅳ.近紫外线对胚 γ_2-晶体蛋白的光氧化作用

对人胚胎晶体γ_2-晶体蛋白溶液用近紫外线(γmax 365nm)照射,不同时间取样分析。利用巯基(-SH)特异性试剂 DACM[N-(7-甲基氨基-4-甲基-3-邻羟苯烯基)-马来酰亚胺],经 SDS-聚丙烯酰胺凝胶电泳观察光照射后的γ_2-晶体蛋白及其聚合物的游离巯基,同时检测照射后的蛋白溶液的紫外吸收光谱、光散射强度、色氨酸荧光发射光谱,以及丙烯酰胺对色氨酸荧光的猝灭效应。结果显示:人胚胎γ_2-晶体蛋白经紫外线照射,使其游离-SH 减少,蛋白聚合和分解,蛋白溶液的光散射增强,其二聚体不含游离-SH,γ_2-晶体蛋白的色氨酸荧光减少,这些变化随照射时间的延长而更明显;而对丙烯酰胺对蛋白分子中的色氨酸荧光猝灭效应影响不大。说明人胚胎γ_2-晶体蛋白通过光氧化,损伤及含游离-SH 的氨基酸残基、色氨酸残基,光氧化促使蛋白聚合和分解.提出通过进一步定量分析,可能在较深层次揭示紫外光氧化损伤含-SH 氨基酸、色氨酸残基,进而影响整个蛋白质分子结构的差异。眼科学报 1993;9:85-89。     

Non-tuberculous mycobacterial keratitis: a study of 22 cases.

AIM: To investigate causes and clinical findings of non-tuberculous mycobacterial keratitis, and to study its response to topical antibiotic therapy and surgical extirpative keratectomy. METHOD: A single centre, retrospective review of 22 patients with non-tuberculous mycobacterial keratitis seen in a 3 year period. Laboratory diagnoses were established with Ziehl-Nielsen acid fast staining and Löwenstein-Jensen cultures. RESULTS: In 20 patients (91%), there was an antecedent history of foreign body eye trauma (18 patients) or elective surgery (two patients). There were 19 cases of Mycobacterium chelonei, and three of M fortuitum. Clinical signs included epithelial defects, satellite or ring stromal infiltrates, crystalline keratopathy, and hypopyon. For topical antibiotic therapy, 20 patients received amikacin, while one patient received rifampin and another received ciprofloxacin, each in accordance with the results of the in vitro drug sensitivities. An extirpative keratectomy was performed in 15 cases; four of these cases additionally required a temporary conjunctival flap in order to finally eradicate the infection. At the end of the follow up period (median 18 months; range 3 months to 3 years) all eyes were stable and free of infection, with 19 (86%) having final visual acuities of 20/200 or better. CONCLUSION: Early clinical recognition and prompt laboratory diagnosis, together with aggressive topical antibiotic therapy and early keratectomy, may shorten morbidity and improve the clinical outcome of non-tuberculous mycobacterial keratitis.     

羊水细胞培养宫内诊断的应用(附120例分析)

报告120例羊膜腔穿刺羊水细胞培养结果:在成功的99例中,发现二例染色体异常,核型为47,xy,+18和46,xx/47,xx,+F,在21例失败中出现一例眼球畸形。培养成功率为82.5%;染色体异常检出率为2.02%。无一例因羊膜腔穿刺而导致流产,但出现一例轻度羊水栓塞合并症。并对羊水细胞培养在产前宫内诊断中应用价值及术后并发症进行了讨论。