Effect of allitridi on calcium current in rat ventricular myocytes

Background Allitridi is an active compound that is extracted from the garlic. It has effects of anti-atherosclerosis, anti-arrhythmias and lowering blood pressure. But the controversy about the effect on cardiac contractility still exists. Methods Whole-cell patch clamp recording technique was used to record ICa,Lin single cell isolated rat ventricular myocytes. The nifedipine- sensitive L-type calcium current was recorded in the rat ventricular myocytes. Results Allitridi decreased the calcium channel current in a dose-dependent and voltage-dependent manner in ventricular myocytes of rats. The current-voltage curve was shifted upwards, on which active potential,peak potential and reverse potential showed no significant changes. The inactivation curve was shifted to more negative potential, but the activation curve and recovery curve were not altered. Allitridi had no effect on frequent-dependency of calcium current. Conclusion These results show that allitridi could concentration-dependently decrease calcium channel current in ventricular myocytes of rats.     

应用Carto系统指导射频消融治疗希氏束附近间隔部心动过速

目的　探讨应用Carto系统指导射频消融(radiofrequencyablation ,RFA)治疗希氏束附近间隔部心动过速 (septaltachycardia ,ST)的价值。方法　 2 1例ST患者 ,包括房室结折返性心动过速 (AVNRT) 12例、间隔部房室旁道(SAP) 4例、房间隔下部房性心动过速 (AT) 5例 ,应用该系统 ,建立三维电解剖图 ,标识希氏束及放电部位 ,并测量两者之间的距离 ,消融时实时观察导管位置、方向。结果　 2 1例均消融成功 ;试放电部位、有效靶点与希氏束之间的距离分别为 (8 9± 3 5 )mm、(8 7± 3 3)mm ;AVNRT12例有效放电过程中均出现间歇性结性心律 ,1例AT心动过速终止后偶见结性逸搏 ;无房室传导阻滞 (AVB)并发症 ;随访 3～ 16个月 ,无心动过速发作。结论　应用Carto系统指导RFA治疗ST ,可清楚显示靶点与希氏束之间的距离 ,实时观察导管的位置及方向 ;通过标识有效放电部位 ,避免出现无效放电 ,降低发生AVB的危险性     

电解剖标测与常规方法射频消融治疗房性快速心律失常的对比研究

电解剖标测与常规方法射频消融治疗房性快速心律失常的对比研究     

The Observation on Efficacy of Angio-seal Closure Device in the Femoral Arterial Puncture Site after PCI

Objective To evaluate reliability and safety of Angio-seal hemostasis device applied to the femoral arterial puncture site after percutaneous coronary intervention (PCI). Methods In 40 patients after PCI in our institute during the period between May 2002 and December 2003, Angio-seal device were used to seal the femoral arterial puncture site. Results All the Angioseal devices were successfully deployed in 40 patients ( successful rate; 100%); the mean time to hemostasis was 45±12 sec; the mean time to ambulate after angioseal closure was 1.9 ?.5 hours. No major groin and systemic complication was observed. There was minor groin oozing in 2 cases and small hemotoma in 1 patient. Conclusions Angio-seal closure device of the femoral artery puncture site after a percutaneous coronary procedure is safe. It can shorten the time to hemostasis, leads to early mobilization, and reduce groin complication. The disadvantage is relatively expensive.     

原发性肺动脉高压合并妊娠1例报告

患者，女性，22岁，初次妊娠30周。1年前出现劳力性气促，妊娠后加重，近17天不能手卧，伴咯鲜血，每次约10～30ml。体检：BP13／7kPa，半坐卧位，无紫组，无颈静脉怒张，肺动脉瓣区第二心音亢进，三尖瓣听诊区闻及收缩期杂音11／VI级，两肺未闻及干湿性罗音。心电图示窦性心动过速，电轴右偏，心脏重度顺钟向转位。二维超声心动图示右房、右室明显增大，分别为52nun、57mm，肺动脉增宽，为29mm，三尖瓣重度关闭不全。胸片示肺动脉隆凸，肺动脉分支增粗，左肺上叶前段肺梗塞。Swan－Ganz飘浮导管监测及血氧饱和度测定提示无房室水平左向…     

先天性心脏病外科手术后切口性房性心动过速三维电磁导管标测及射频消融

目的；探讨天先性心脏病外科手术后切口性房性心动过速（房速）三维电磁导管（即Carto)标测特点及射频消融价值。方法：5例切口性房速患者，应用Carto系统标则右心房，实时重建心腔三维电解剖图，标识瘢痕区，观察电热图，传导图，于折返所经过的关键峡部位线性消融，结果：4例房速呈持续性，1例（三房心）术中不能诱发，电势图示低电压区主要分布于右房游离壁，在右房中侧壁下侧壁分别标测到瘢痕区1（S1）和瘢痕区2（S2）。4例持续性房速发生机理与折返有关，折返环位于低电压区，中心解剖障碍区均为中侧壁瘢痕区1，3例折返经过S1－S2，1例经过S1－三尖瓣环之间的关键峡部，于上述关键峡部消融，均获成功，三房心患者（未诱导心动过速）于S1－S及三尖瓣环－下腔静脉之间行线性消融，无并发症，随访2－24个月，其中1例（三房心）于术后1个月出现不典型心房扑动，结论：提示先天性心脏病术后房速的发生机理与折返有关，应用Carto系统标测可清楚地显示折返途径，消融折返所经过的关键峡部可望达到根治目的。     

治疗浓度的雷诺嗪对人心房肌细胞电活动的影响

目的:探讨治疗浓度的雷诺嗪对人心房肌细胞电活动的影响。方法:从冠状动脉旁路移植术患者右心耳分离心房肌细胞,在治疗浓度的雷诺嗪作用下,使用膜片钳技术记录动作电位(APD)及峰钠电流、L型钙通道电流(LCa,L)、瞬时外向钾电流(Ito)、超快速延迟整流钾电流(IKur),观察给药前后的变化。结果:10μmol/L雷诺嗪使APD50和APD90分别延长27.5%和14.2%,而对静息电位和动作电位的幅度无明显影响;使钠电流失活曲线V0.5从(-92.9±7.8)mV左移至(-103.5±3.9)mV,对ICa,L、Ito、IKur的电流-电压曲线无明显影响。结论:10μmol/L雷诺嗪延长APD50和APD90,并使快钠通道失活增加。     

致心律失常性右室心肌病高危患者相关危险因素分析

目的探讨致心律失常性右室心肌病(ARVD/C)高危患者相关危险因素。方法根据1994年ARVD/C诊断标准,纳入43例ARVD/C先证者。分组标准:有晕厥病史并记录到室性心动过速(简称室速)为高危病人;记录到室性早搏(简称室早)、室速但无晕厥病史及其他临床情况定为低危病人。收集参数包括:①心电图V1～3QRS波时限≥110 ms、V1～3导联S波升支时限≥55 ms、Epsilon波、T波倒置、(V1+V2+V3)/(V4+V5+V6)QRS波时限≥1.2、QRS波离散度≥40 ms、QT离散度≥65 ms;②信号平均心电图记录晚电位参数;③Holter记录室早或室速;④超声记录双房、双室及右室流出道、流入道内径大小。Logistic回归分析高危患者ARVD/C病人的相关危险因素。结果心室晚电位阳性、右室射血分数<0.40与高危ARVD/C显著相关。结论晚电位阳性、右心功能不全是ARVD/C的高危因素。     

肌球蛋白重链7基因来源的miR-208b-3p促进心肌成纤维细胞中纤维化相关基因表达

【目的】探究肌球蛋白重链7基因来源的微小RNA-208b-3p(miR-208b-3p)对心肌成纤维细胞纤维化表型的调控作用。【方法】通过miRNA表达谱芯片分析18周龄的糖尿病db/db小鼠和db/m对照小鼠心肌中差异的miRNAs。通过实时荧光定量PCR(RT-qPCR)检测miR-208b-3p在血管紧张素Ⅱ（AngⅡ）和高糖/葡萄糖氧化酶（G/Go）处理的C57BL/6乳小鼠心肌细胞（NMVCs）和心肌成纤维细胞（mCFs）中的表达;利用CCK8细胞增殖实验、流式细胞术和纤维化相关蛋白（包括COL1A1、COL3A1和α-SMA）表达检测来了解miR-208b-3p对mCFs纤维化表型的影响;双萤光素酶报告基因实验检测miR-208b-3p与潜在靶基因Mtf2和Pgrmc1 3′端非翻译区（3′-UTR）的结合作用;利用RT-qPCR和Western blot法分别检测miR-208b-3p转染的mCFs中Mtf2和Pgrmc1表达;通过小干扰RNA(siRNA)抑制Mtf2和Pgrmc1表达,并检测对mCFs中纤维化相关蛋白表达的作用。【结果】miRNA表达谱和RTqPCR结...