制膏 30年规范不断创新

一谈到中医膏方．人们习惯想到药店可买到的北瓜膏、十全大补膏、葆春膏等。这类传统膏方由专门厂家生产具有国家批准的药品生产批准文号．处方固定，疗效确切，批量生产被称为成方膏方。     

干扰素栓联合微波治疗宫颈糜烂疗效观察

慢性宫颈炎是女性生殖系统最为常见的炎症性疾病,约有50%育龄妇女患有此病,也是诱发宫颈癌的高危因素[1]。近年来的研究提示宫颈糜烂的发生可能与单纯疱疹病毒、人乳头状瘤病毒的感染密切相关[2]。宫颈糜烂采用微波治疗效果虽好,但存在术后水样白带多、创面愈合时间较长的缺点。2002年1月—2003年12月,本院采用干扰素栓加微波治疗宫颈糜烂取得良好效果,现报道如下。1临床资料1·1一般资料选择上述时期在本院就诊的宫颈糜烂患者132例,年龄24~52岁,平均36岁。治疗前常规白带检查排除滴虫、霉菌感染,且宫颈刮片或活组织病理检查排除恶性病变。…     

他莫昔芬对单侧输尿管梗阻大鼠肾间质纤维化的保护作用

目的:探讨他莫昔芬对肾组织纤维化的影响及作用机制.方法:雌性SD大鼠,建立单侧输尿管梗阻模型(UUO),设:①模型组(n=6),行左侧输尿管结扎术,0.3 ml无菌生理盐水灌胃,每天2次;②治疗组(n=6),他莫昔芬5 rmg/kg(0.3 ml无菌生理盐水配置)灌胃,每天2次;③假手术组(n=6).术后第9天处死各组大鼠.采用光镜和免疫组化观察肾组织纤维化和α平滑肌肌动蛋白(α-SMA)表达的变化.结果:①治疗组大鼠肾间质纤维化程度较模型组明显减轻.②UUO模型第9天时大鼠肾组织α-SMA表达明显增强,采用他莫昔芬治疗后,肾组织α-SMA表达的异常增强能得到有效抑制.结论:昔芬类药物具有减轻肾组织纤维化的作用,而这一作用与其能有效阻抑肾组织上皮细胞-间充质细胞转分化(EMT)有关.     

HPLC法测定调中生血丸中橙皮苷的含量

调中生血丸是上海岳阳中西医结合医院研制的治疗缺血性贫血的纯中药制剂，由降矾和陈皮两味中药组成，组方简明、目标明确。以降矾补铁，重用陈皮，理气健脾，减少补铁药降矾对肠胃的刺激作用，调中和胃促进铁的吸收，达到补血的功效。为有效控制制剂的质量，作采用了HPLC法测定调中生血丸中的主要有效成分橙皮苷的含量。     

老年急性脑血管病合并多器官功能衰竭血浆中ET和CGRP变化的意义

目的检测老年急性脑血管病(CVD)并发多器官功能衰竭(MOF)患者血浆ET-1、CGRP的水平,探讨其在疾病发生和治疗中的作用。方法采用放免分析法检测脑出血(CH)、脑梗死(CI)、MOF和对照组血浆中ET-1、CGRP水平。结果MOF组血浆中ET-1(89.73±21.42pg/ml)和CGRP(63.71±18.79pg/ml)显著高于非MOF CVD组和正常对照组,P<0.01。MOF在CH组占93%,在CI组占50.94%,P<0.05。在不同的防治时间与疾病预后的研究中发现,早期防治组发生MOF以25%低于非早期防治组MOF的91%,且预后好,P<0.05。结论(1)ET-1增高可能在引起MOF中起一定作用。(2)对ET-1和CGRP增高者应早期采取有效的防治措施方可降低MOF的发生率。     

Expression of the novel gene NM23-H1B in ovarian cancer

Objective：To study the expression of the human novel gene NM23-H1B in ovarian cancer.Methods: Totally 24 samples from patients with epithelial ovarian tumor at different clinical stages and 4 from normal ovaries were examined for NM23-H1B mRNA expression by RT-PCR and Northern blot. Results: All samples expressed NM23-H1B mRNA through RT-PCR, while the level of expression in ovarian tumor was higher than that of normal ovary. The results of Northern blot showed that NM23-H1B was overexpressed in ovarian cancer while lowexpressed in normal ovary or low malignant potential (LMP).The level of expression at early stage cancer (stage Ⅰ and Ⅱ ) was higher than those in advanced cancer(stage Ⅲ and Ⅳ ). In early stage carcinoma, the expression level was involved in the differentiation of tumor cell, and well-differentiated cancer expressed NM23-H1B mRNA in comparatively higher level. Conclusion: The novel gene NM23 N1B is closely correlated with the ovarian cancer.     

复方二精灵多糖对谷氨酸诱导的海马神经元凋亡的保护作用

目的探讨中药复方二精灵多糖(EJL)预防阿尔茨海默病的机制。方法实验分为正常对照组、模型(100μmol.L-1谷氨酸诱导)组、阳性对照组(50μmol.L-1多奈哌齐)及EJL实验组(0.5,1.0,2.0及3.0g.L-1)。体外培养9d的海马神经元先用各药物预处理一定时间后,加入谷氨酸。采用MTT法、Hoechst 33258荧光染色、流式细胞术和Western蛋白质印迹法等方法检测细胞凋亡。结果MTT检测发现,随着EJL浓度的增加,细胞的存活率也增加;与模型组〔(59.6±4.6)%〕比较,EJL2.0及3.0g.L-1组细胞存活率明显升高〔(82.8±2.8)%和(89.4±4.1)%;n=3,P<0.05〕。荧光染色观察形态学变化发现,EJL预处理组凋亡细胞明显减少。FITC-Annexin Ⅴ和PI双染,在直方图中可以发现,模型组的凋亡率为32.33%,EJL预处理组则分别为24.33%,22.01%及12.12%。Western蛋白质印迹法结果显示,与模型组比,EJL2.0g.L-1预处理可以显著提高Bcl-2蛋白表达量,减少Bax蛋白表达量。结论EJL对海马神经元有一定的保护作用。     

慢性盆腔炎的中医治疗临床观察

本文统计了2005年—2009年3年中我院妇产科门诊应用中西医结合的方法治疗慢性盆腔炎80例疗效观察,发现中药重楼汤、红藤败酱汤、乌头暖宫散在治疗慢性盆腔炎时通过不同途径给药连续治疗1—5个疗程,平均3个疗程,治愈率85%,基本治愈10%,好转5%,总有效率100%。结果提示,采用中医辨证施治手段,运用中医的清热利湿、活血化瘀、软坚消症之法必要时配以西药治疗,能明显提高慢性盆腔炎的治愈率。     

小切口超薄皮瓣法治疗腋臭180例体会

腋臭是美容门诊的常见病，在我国发病率高达6．41％[11。目前用于治疗腋臭的手术方法很多，但各有利弊。我科2000年4月-2007年6月采用小切口超薄皮瓣法治疗腋臭180例，效果较好，现报道如下。 1 资料与方法 1．1 临床资料本组共180例，其中男46例，女134例；年龄14～45岁，平均25岁。均为双侧发病，其中8例患者曾在外科行过单侧或双侧腋毛区切除术，     

一种定量评估化学物质致癌性的新方法：比值评估法

Based upon the results of battery of mutagenesis tests, a new approach, ratio estimate method (REM), for predicting carcinogenicity of chemicals quantitatively is described. If the tests are composed of n mutually independent experiments, a result's set is constituted:M = [M1,M2,..., Mn]. According to the formulas of probability, we have: P (C+/M)/P(C-/M) = [P(C+)/P(C-)]. (formula; see text) where P(C+/M)/P(C-/M) is the ratio of the probability of a carcinogen to a non-carcinogen with the result's set of M. P(C+) and P(C-) signify the probability of a carcinogen and a non-carcinogen. P(Mi/C+) and P(Mi/C-) represent the probability with which the result Mi can be obtained to a carcinogen or a non-carcinogen. Let (formula; see text) then beta = gamma/alpha. The value gamma can be derived from the sensitivity and specificity of mutagenesis tests. It is difficult to get the exact value of alpha but one can give its suitable range. For example, let a less than or equal to alpha less than or equal to b, then gamma/gamma + b less than or equal to P(C+/M less than or equal to gamma/gamma + a. Thus, the mean value P(C+/M) = gamma/2 (1/gamma + b + 1/gamma + a) could be taken as an estimated probability to predict the carcinogenicity. The application of REM to predict the carcinogenicity of a carcinogenic and a non-carcinogenic polycyclic aromatic hydrocarbon was illustrated. The error range of the value estimated by REM was also discussed in detail. The major advantage of REM is that it only demands to give an experiential range of P(C-)/P(C+) without pre-determining its exact value and the calculating process is simple and feasible to the toxicologists.