Effects of cadmium, zinc and nitrogen status on non-protein thiols in the macroalgae Enteromorpha spp. from the Scheldt Estuary (SW Netherlands, Belgium) and Thermaikos Gulf (N Aegean Sea, Greece)

Enteromorpha prolifera (Scheldt Estuary) and E. linza (Thermaikos Gulf) were incubated at three salinities with 100 and 200microgL(-1)Cd and Zn. The objective was to measure effects of Cd, Zn and nitrogen (N) status on the pools of metal-binding non-protein thiols: glutathione and phytochelatins, (gamma-glutamyl-cysteinyl)(n)-glycine (PC). In E. linza, ammonium pools were higher, but amino acid pools, total N and protein contents were lower than in E. prolifera. Reduced glutathione (GSH) pools were positively correlated with free glutamate and protein contents. In E. linza GSH pools increased and the ratio of reduced to oxidized glutathione (GSH:(GSH+0.5GSSG)), an indicator of oxidative stress, decreased with Cd contents, indicating Cd-induced glutathione oxidation. Total glutathione pools (reduced plus oxidized) ranged from 16nmolSgdwt(-1) in controls (at 0.5micromolCdgdwt(-1)) to 179nmolSgdwt(-1) (at 1.9micromolCdgdwt(-1)) at the highest cadmium dosage. Cadmium stimulated PC synthesis in E. prolifera which suggests that in N-rich algae, glutathione pools were high enough for PC synthesis. In both species GSH and protein increased with Zn contents, whereas GSH:(GSH+0.5GSSG) decreased, which would indicate Zn-induced oxidative stress; in E. linza, at the highest salinity the glutathione redox ratio decreased from 0.61 (at 2.9micromolZngdwt(-1)) to 0.26 (at 4.9nmolSgdwt(-1)) (at 0.5molCdgdwt(-1)). PCs were not synthesized in response to Zn, which may have resulted in Zn-induced GSH oxidation. The presence of both oxidative effects (Cd, Zn) and detoxification (Cd) could be identified by observing the responses of glutathione and PC pools to metal stress.     

Evaluation of the Presence of Methane in Złoczew Lignite: Comparison with Other Lignite Deposits in Poland

Natural Resources Research - Catastrophic cases of methane explosion during exploratory drilling within the Be?chatów ortholignite deposit have led to testing for methane in other Polish...     

Joint modelling of wave spectral parameters for extreme sea states

Characterising the dependence between extremes of wave spectral parameters such as significant wave height (H_S) and spectral peak period (T_P) is important in understanding extreme ocean environments and in the design and assessment of marine structures. For example, it is known that mean values of wave periods tend to increase with increasing storm intensity. Here we seek to characterise joint dependence in a straightforward manner, accessible to the ocean engineering community, using a statistically sound approach.Many methods of multivariate extreme value analyses are based on models which assume implicitly that in some joint tail region each parameter is either independent of or asymptotically dependent on other parameters; yet in reality the dependence structure in general is neither of these. The underpinning assumption of multivariate regular variation restricts these methods to estimation of joint regions in which all parameters are extreme; but regions where only a subset of parameters are extreme can be equally important for design. The conditional approach of Heffernan and Tawn (2004), similar in spirit to that of Haver (1985) but with better theoretical foundation, overcomes these difficulties.We use the conditional approach to characterise the dependence structure of H_S and T_P. The key elements of the procedure are: (1) marginal modelling for all parameters, (2) transformation of data to a common standard Gumbel marginal form, (3) modelling dependence between data for extremes of pairs of parameters using a form of regression, (4) simulation of long return periods to estimate joint extremes. We demonstrate the approach in application to measured and hindcast data from the Northern North Sea, the Gulf of Mexico and the North West Shelf of Australia. We also illustrate the use of data re-sampling techniques such as bootstrapping to estimate the uncertainty in marginal and dependence models and accommodate this uncertainty in extreme quantile estimation.We discuss the current approach in the context of other approaches to multivariate extreme value estimation popular in the ocean engineering community.     

Optimization of two GPS/MEMS-IMU integration strategies with application to sports

The application of low-cost L1 GPS receivers integrated with micro-electro-mechanical system (MEMS) inertial measurement units (IMU) allows the continuous observation of position, velocity and orientation which opens new possibilities for comparison of athletes’ performance throughout a racecourse. In this paper, we compare loosely and closely coupled integration strategies under realistic racing scenarios when GPS is partially or completely masked. The study reveals that both integration approaches have a similar performance when the satellite constellation is completed or the outages are short. However, for less than four satellites, the closely coupled strategy clearly outperforms the loosely coupled approach. The second part of the paper is devoted to the important problem of system initialization, because the conventional GPS/IMU alignment methods are no longer applicable when using MEMS-IMU. We introduce a modified coarse alignment method and a quaternion estimation method for the computation of the initial orientation. Simulations and practical experiments reveal that both methods are numerically stable for any initial orientation of the sensors with the error characteristics of MEMS-IMUs. Throughout the paper, our findings are supported by racing experiments with references provided in both, the measurement and the navigation domains.     

采点和去趋势方法对天山西部云杉上树线树轮宽度年表相关性及其气候信号的影响

针对新疆天山西部伊犁、博州三大山体北坡云杉上树线树木年轮资料,采用区域曲线、负指数曲线、样条函数三种不同生长去趋势方法,研制树轮宽度年表,分析不同采点和树轮去趋势方法对树轮宽度年表在不同频域的相关性及气候信号的影响发现：①采点间的距离和是否位于同一气候区是影响云杉上树线树轮宽度年表间相关性的首要因子。三个采点间的树轮宽度变化,在高频变化方面最为相似,低频变化存在着较大差异,表明它们在揭示和重建大范围的高频气候变化方面可能具有较大的应用潜力。②三种不同去趋势方法对天山西部山脉北坡上树线的云杉树轮宽度年表的研制具有一定影响,负指数和样条函数去趋势方法研制的树轮年表较为相似,而区域曲线去趋势方法研制的树轮宽度年表中则可保留较多的轮宽低频变化信息。③降水是天山西部云杉上树线的树轮宽度生长的主要限制因子,且树轮宽度生长对降水的响应具有显著的滞后性。④在天山西部的云杉上树线采点,使用区域曲线去趋势方法有利于在树轮宽度年表中保留较多的与水分有关的气候信息。     

For your eyes only? Evaluating a coordinated and multiple views tool with a map,a parallel coordinated plot and a table using an eye-tracking approach

Geographic visualization tools with coordinated and multiple views (CMV) typically provide sets of visualization methods. Such configuration gives users the possibility of investigating data in various visual contexts; however, it can be confusing due to the multiplicity of visual components and interactive functions. We addressed this challenge and conducted an empirical study on how a CMV tool, consisting of a map, a parallel coordinate plot (PCP), and a table, is used to acquire information. We combined a task-based approach with eye-tracking and usability metrics since these methods provide comprehensive insights into users’ behaviour. Our empirical study revealed that the freedom to choose visualization components is appreciated by users. The individuals worked with all the available visualization methods and they often used more than one visualization method when executing tasks. Different views were used in different ways by various individuals, but in a similarly effective way. Even PCP, which is claimed to be problematic, was found to be a handy way of exploring data when accompanied by interactive functions.     

Measuring the significance of a divide to local drainage patterns

This article presents a framework for estimating a new topographic attribute derived from digital elevation models (DEMs) called maximum branch length (B _max). Branch length is defined as the distance travelled along a flow path initiated at one grid cell to the confluence with the flow path passing through a second cell. B _max is the longest branch length measured for a grid cell and its eight neighbours. The index provides a physically meaningful method for assessing the relative significance of drainage divides to the dispersion of materials and energy across a landscape, that is, it is a measure of ‘divide size’. B _max is particularly useful for studying divide network structure, for mapping drainage divides, and in landform classification applications. Sensitivity analyses were performed to evaluate the robustness of estimates of B _max to the algorithm used to estimate flow lengths and the prevalence of edge effects resulting from inadequate DEM extent. The findings suggest that the index is insensitive to the specific flow algorithm used but that edge effects can result in significant underestimation along major divides. Edge contamination can, however, be avoided by using an appropriately extensive DEM.     

Palaeoclimatic studies in South Shetland Islands,Antarctica, based on numerous stratigraphic variables in lake sediments

The hitherto longest found lake sediment sequence on Byers Peninsula, Livingston Island, South Shetland Islands, was analysed with respect to lithology, chronology, diatoms, Pediastrum, pollen and spores, mosses, mineralogy, and sediment chemistry. During the ca. 5000 year long development the sediments were influenced by frequent tephra fall-outs. This volcanic impact played a major role in the lake's history during two periods, 4700–4600 and 2800–2500 BP, but was of importance during the lake's entire history with considerable influence on many of the palaeoenvironmentally significant indicators. The large and complex data set was analysed and zonated with different types of multivariate analysis. This resulted in a subdivision of the sequence into 8 time periods and 21 variables. Redundancy analysis (RDA) of this data set, both without and with the tephra periods, and with 4–6 of the variables as explanatory environmental variables, reveal that climatic/environmental signals are detectable. The palaeoclimatic picture that emerged out of the tephra noise suggests that the first 100 years were characterized by mild, humid conditions. This was followed by a less mild and humid climate until ca. 4000 BP when a gradual warming seems to have started, coupled with increased humidity. These mild and humid conditions seem to have reached an optimum slightly after 3000 BP. At ca. 2500 BP a distinct climatic deterioration occurred with colder and drier conditions and long seasons with ice cover. This arid, cold phase probably reached its optimum conditions at ca. 1500 BP, when slightly warmer conditions might have prevailed for a while. Except for the modern sample with rather mild climate, the last 1400 years seem to have been fairly arid and cold, and the effects of the frequent volcanic activity during this period is only vaguely seen in the records.