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101.
Protein import into the nucleus is generally considered to involve specific nuclear localization signals (NLS) though it is becoming increasingly clear that efficient and well controlled import of proteins which lack a canonical NLS also occurs in cells. Human immunodeficiency virus type 1 (HIV-1) Vpr is one such protein which does not have an identifiable canonical NLS and yet efficiently localizes to the nuclear compartment. Here, we use confocal microscopy to demonstrate that mutations in the putative central hydrophobic helix of Vpr result in the retention of the protein in highly localized ring-like structures around the nuclear periphery with striking impairment in their ability to enter the nuclear interior. By characterizing other biological activities associated with this protein, such as its ability to incorporate into budding virions and its ability to arrest cells in G2, we show that this helical domain is specific for the nuclear translocation of the protein with very little effect on these other functions. Interestingly, however, perturbation of this helical motif also perturbs the protein's ability to augment viral replication in primary human macrophages indicating that the integrity of this secondary structure is essential for optimal infection in these non-dividing cells. 相似文献
102.
Chiyoko Horike Kazuki Morita Toru H. Okabe 《Metallurgical and Materials Transactions B》2012,43(6):1300-1307
Platinum (Pt) is typically recovered by employing dissolution processes in aqueous solutions; however, these processes require a long processing time and considerable quantities of acids with strong oxidants owing to the high chemical stability of Pt. In order to develop an efficient dissolution process, we studied chlorination treatments for Pt prior to dissolution. Chlorination was carried out at 673?K to 873?K (400?°C to 600?°C) using copper(II) chloride (CuCl2) as a chlorine source. While pure Pt was insoluble in hydrochloric acid (HCl(aq)), the entire Pt component of the treated sample dissolved in HCl(aq) under certain conditions. Therefore, the proposed method can be used as a new, environmental friendly Pt recovery process. 相似文献
103.
Masago Y Katayama H Watanabe T Haramoto E Hashimoto A Omura T Hirata T Ohgaki S 《Environmental science & technology》2006,40(23):7428-7433
Noroviruses are one of the major causes of viral gastroenteritis in Japan. A quantitative risk assessment was conducted to evaluate the health risk caused by this virus in drinking water. A Monte Carlo analysis was used to calculate both the probability of infection and the disease burden using disability-adjusted life years (DALYs). The concentration of noroviruses in tap water was estimated based on qualitative data and a most probable number (MPN) method with an assumed Poisson lognormal distribution. This numerical method was evaluated using two sets of available count data of Cryptosporidium: that collected from a river and that found in tap water in Japan. The dose-response relationships for noroviruses were estimated using assumed ID50 (10 or 100). The annual risk was higher than the US-EPA acceptable level (10(-4) [infection/ person-year]) but around the WHO level (10(-6) [DALYs/ person-year]). As suggested by others, since microbial concentrations are generally lognormally distributed, the arithmetic mean was directly related to the annual risk, suggesting that the arithmetic mean is more useful in representing the degree of microbial contamination than the geometric mean. 相似文献
104.
Tomakomai Large Scale Crude Oil Fire Experiments 总被引:6,自引:0,他引:6
Hiroshi Koseki Yusaku Iwata Yasutada Natsume Toru Takahashi Toshisuke Hirano 《Fire Technology》2000,36(1):24-38
This paper summarizes the results of large-scale crude oil fire experiments conducted in Tomakomai, Japan, in 1998 to obtain information that could be applied to the development of firefighting strategies for, and the design of, huge petroleum storages. Arabian light-equivalent crude oil was burned in pans 5-, 10-, and 20-m in diameter. Most of the experiments were performed under favorable conditions. Measured data include external radiation, infrared image of the flame, flame temperature, gas concentration inside the flame, and other burning characteristics. The height of the strongest radiant emittance was H/D=0.1 to 0.2, where D=pan diameter and H=height from the initial fuel surface, and a kind of fireball appeared occasionally at the intermittant flame zone. Emitted smoke particles were sampled on the ground and observed with a scanning electron micrograph, and the distribution of the diameters of primary smoke particles was examined. The average diameter of primary smoke particles is 53.0 (±10.5)nm. The dependence of burning characteristics and flame structure on pan diameter is discussed. The flame height of the 20-m diameter pan fire is 1.9 (±0.3) D. The burning rate increases as the pan diameters increase, but the radiative fraction decreases as pan diameter increases. 相似文献
105.
Hideyuki Nakano Toru Takahashi Takahiro Tanino Yasuhiko Shirota 《Dyes and Pigments》2010,84(1):102-107
Novel azobenzene-based photo-responsive amorphous molecular materials, 4-[bis(9,9-dimethylfluoren-2-yl)amino]-4′-cyanoazobenzene and 4-[bis(9,9-dimethylfluoren-2-yl)amino]-4′-nitroazobenzene, have been synthesized and the formation of surface relief grating on their amorphous films has been investigated. It was found that a relatively large surface relief grating could be inscribed on both amorphous films upon interference exposure to the writing laser beams. The modulation depth of the surface relief grating inscribed on the amorphous film of the cyano-substituted material was found to be larger than that inscribed on the film of the nitro-substituted one and seemed to be comparable to that inscribed on the amorphous film of the parent material, 4-[bis(9,9-dimethylfluoren-2-yl)amino]azobenzene. These results were discussed from the viewpoint of their trans–cis photoisomerizations as amorphous films and glass-transition temperatures. 相似文献
106.
107.
The enantiomeric composition of the monounsaturated triacylglycerols (TG) from cocoa butter was estimated. The monounsaturated
TG were separated into three fractions by reversed-phase high-performance liquid chromatography (HPLC), and each fraction
was subjected to the stereospecific analysis with chiral-phase HPLC. The results indicated that the major TG consisted of
equal amounts of 1-stearoyl-2-oleoyl-3-palmitoyl-sn-glycerol (SOP-sn-TG) and POS-sn-TG (47 mol%), 1,3-distearoyl-2-oleoyl-glycerol (SOS-TG) (33 mol%), and POP-TG (19 mol%). The contents of SOP-sn-TG and POS-sn-TG are 1.30 times that of the POP-TG content, and the SOS-TG content is 1.302 times that of the POP-TG content. The term “priority factor” is proposed for the ratio of the stearoyl group/palmitoyl group,
1:30 at thesn-1 andsn-3 or 1(3)-position. It shows a distinct specificity for particular fatty acids or their Coenzyme A esters in random esterification
at each position of the glycerol moiety in the biosynthesis of cocoa butter TG. 相似文献
108.
Ippei Takasaki Toshiaki Nagumo Toru Inaba Nobuyuki Yoshino Tadashi Maruyama 《Journal of Nuclear Science and Technology》2013,50(8):867-872
This article describes the method for measuring the isotopic abundance of 10B in nuclear grade boron carbide using inductively coupled plasma-quadrupole mass spectrometry (ICP-QMS). The results of investigation revealed that both the integration time and the dwell time have a major influence on the reproducibility of ICP-QMS measurements. As a result of optimization of the measurement conditions, reproducibility below 0.2% relative standard deviation (RSD) (0.17% RSD maximum) was achieved. In addition, the measured value of the isotopic abundance of 10B for each sample well agreed with the values measured by the TIMS. Thus, the method described in the present investigation was very effective in the analysis of isotopic abundance of 10B in B4C or H3BO3. The results of this study suggest that ICP-QMS could be applied to the precise analysis of the isotopic abundance of 10B required in the field of nuclear applications. 相似文献
109.
A recirculating system of aqueous trichloroethylene (TCE) solutions through the packed bed reactor with TiO2 pellets has been developed in order to mineralize TCE without difficulties for filtration and recovery of catalyst. The TiO2 pellets prepared by sol gel method have photocatalytic activity similar to commercially available PC-101 and PC-102 in the powder form and to ST-B11 pellets. In batch experiments with TiO2 powders, Degussa P-25 is the most active photocatalyst, which indicates that specific surface area is not an important factor controlling the photocatalytic activity in aqueous solutions. The degradation rates of TCE in the recirculating system with TiO2 pellets decreased in the presence of H2O2, while were remarkably accelerated by adding S2O8(2-). The presence of S2O8(2-) ions more than 0.01 mol dm(-3) completely suppressed hole-electron recombination and mineralized 50 ppm TCE with the 2 h irradiation. In a reactor without TiO2 photocatalysts, TCE was photodegraded by SO4- radicals which produced by photodissociation of S2O8(2-). The degradation rates increased with increase of the initial S2O8(2-) concentration. However, TCE was not mineralized but converted to intermediates which were slowly degraded to Cl- by continuing the irradiation. 相似文献
110.