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911.
The full second‐law analysis, developed in Part 1 (M. Pons and A. Kodama, Int. J. Energy Res. 2000; 24 : 251–262) is applied to experimental results. That approach takes into account the irreversibilities due to the open character of the cycle. Measurements are performed on a solid desiccant cooling unit operated in the ventilation mode. Experimental data permit us to establish the entropy balance of the unit. The results show that the sum of all the considered entropy productions completely explain the difference between the Carnot COP and the actual COP of the unit. The effects of three experimental parameters are investigated: the rotation speed of the dehumidifier (desiccant wheel), the air velocity and the regeneration temperature. Experimental results show that there exists an optimal rotation speed which results from a combination between the different entropy productions in the cycle. When the air velocity is increased, together with an accordingly optimized rotation speed, the cooling capacity increases while the COP decreases due to increases in the entropy productions in the dehumidifier and sensible heat exchanger. Moreover, it appears that the most significant entropy productions take place in the dehumidifier and heating system. In the investigated experimental unit, these two entropy productions have similar magnitudes. However, when the regeneration temperature is increased, the irreversibilities due to mass exchanges with outside air become comparable to these and surely should not be forgotten in a global optimization of the process. The present analysis is a solid basis for reducing the largest entropy productions thus optimizing the process. Copyright © 2000 John Wiley & Sons, Ltd. 相似文献
912.
Sumio Michnick Jean-Louis Roustan Fabienne Remize Pierre Barre Sylvie Dequin 《Yeast (Chichester, England)》1997,13(9):783-793
The possibility of the diversion of carbon flux from ethanol towards glycerol in Saccharomyces cerevisiae during alcoholic fermentation was investigated. Variations in the glycerol 3-phosphate dehydrogenase (GPDH) level and similar trends for alcohol dehydrogenase (ADH), pyruvate decarboxylase and glycerol-3-phosphatase were found when low and high glycerol-forming wine yeast strains were compared. GPDH is thus a limiting enzyme for glycerol production. Wine yeast strains with modulated GPD1 (encoding one of the two GPDH isoenzymes) expression were constructed and characterized during fermentation on glucose-rich medium. Engineered strains fermented glucose with a strongly modified [glycerol] : [ethanol] ratio. gpd1Δ mutants exhibited a 50% decrease in glycerol production and increased ethanol yield. Overexpression of GPD1 on synthetic must (200 g/l glucose) resulted in a substantial increase in glycerol production (×4) at the expense of ethanol. Acetaldehyde accumulated through the competitive regeneration of NADH via GPDH. Accumulation of by-products such as pyruvate, acetate, acetoin, 2,3 butane-diol and succinate was observed, with a marked increase in acetoin production. © 1997 John Wiley & Sons, Ltd. 相似文献
913.
914.
Bun-Ichiro Ono Kazuyasu Kijima Nobuya Ishii Takahiro Kawato Akio Matsuda Andrzej Paszewski Sumio Shinoda 《Yeast (Chichester, England)》1996,12(11):1153-1162
We examined how the activity of O-acetylserine and O-acetylhomoserine sulphydrylase (OAS/OAH) SHLase of Saccharomyces cerevisiae is affected by sulphur source added to the growth medium and genetic background of the strain. In a wild-type strain, the activity was repressed if methionine, cysteine or glutathione was added to the growth medium. However, in a strain deficient of cystathionine γ-lyase, cysteine and glutathione were repressive, but methionine was not. In strains deficient of serine O-acetyltransferase (SATase), OAS/OAH SHLase activity was low regardless of sulphur source and was further lowered by cysteine and glutathione, but not by methionine. From these observations, we concluded that S-adenosylmethionine should be excluded from being the effector for regulation of OAS/OAH SHLase. Instead, we suspected that S. cerevisiae would have the same regulatory system as Escherichia coli for sulphate assimilation; i.e. cysteine inhibits SATase to lower the cellular concentration of OAS which is required for induction of the sulphate assimilation enzymes including OAS/OAH SHLase. Subsequently, we obtained data supporting this speculation. 相似文献
915.
Yuki Hirose Tomo Ohno Dr. Sefan Asamitsu Kaori Hashiya Dr. Toshikazu Bando Prof. Dr. Hiroshi Sugiyama 《Chembiochem : a European journal of chemical biology》2022,23(2):e202100533
Abnormally expanded CAG/CTG repeat DNA sequences lead to a variety of neurological diseases, such as Huntington's disease. Here, we synthesized a cyclic pyrrole-imidazole polyamide (cPIP), which can bind to the minor groove of the CAG/CTG DNA sequence. The double-stranded DNA melting temperature (Tm) and surface plasmon resonance assays revealed the high binding affinity of the cPIP. In addition, next-generation sequencing showed that the cPIP had high specificity for its target DNA sequence. 相似文献
916.
Masafumi Yamaguchi Yoshiro Ohmachi Takahiko Oh'hara Yoshiaki Kadota Mitsuru Imaizumi Sumio Matsuda 《Progress in Photovoltaics: Research and Applications》2001,9(3):191-201
GaAs single‐junction and InGaP/GaAs multi‐junction thin‐film solar cells fabricated on Si substrates have great potential for high‐efficiency, low‐cost, lightweight and large‐area space solar cells. Heteroepitaxy of GaAs thin films on Si substrates has been examined and high‐efficiency GaAs thin‐film solar cells with total‐area efficiencies of 18·3% at AM0 and 20·0% at AM 1·5 on Si substrates (GaAs‐on‐Si solar cells) have been fabricated. In addition, 1‐MeV electron irradiation damage to GaAs‐on‐Si cells has been studied. The GaAs‐on‐Si cells are found to show higher end‐of‐life efficiency than the conventional GaAs cells fabricated on GaAs substrates (GaAs‐ on‐GaAs cells) under high‐fluence 1‐MeV electron irradiation of more than 1 × 1015 cm−2. The first space flight to make use of them has been carried out. Forty‐eight 2 × 2 cm GaAs‐on‐Si cells with an average AM0 total‐area efficiency of 16·9% have been evaluated in the Engineering Test Satellite No.6 (ETS‐VI). The GaAs‐on‐Si cells have been demonstrated to be more radiation‐resistant in space than GaAs‐on‐GaAs cells and 50, 100 and 200‐μm‐thick Si cells. These results show that the GaAs‐on‐Si single‐junction and InGaP/GaAs‐on‐Si multi‐junction cells have great potential for space applications. Copyright © 2001 John Wiley & Sons, Ltd. 相似文献