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71.
Cells employ proteins to perform metabolic functions and maintain active physiological state through charge transfer and energy conversion. These processes are carried out in a narrow space precisely and rapidly, which, no doubt, bring great difficulty for their detection and dissection. Fortunately, in recent years, the development and expansion of single-molecule technique in protein research make monitoring the dynamical changes of protein at single-molecule level a reality, which also provides a powerful tool for the further exploration of new phenomena and new mechanisms of life activities. This paper aims to summarize the working principle and essential achievements of single-molecule technique in protein research in recent five years. We focus on not only dissecting the difference of nanopores, atomic force microscope, scanning tunneling microscope, and optical tweezers technique, but also discussing the great significance of these single-molecule techniques in investigating intramolecular and intermolecular interactions, electron transport, and conformational changes. Finally, the opportunities and challenges of the single-molecule technique in protein research are discussed, which provide a new door for single-molecule protein research. 相似文献
72.
Long Tang Jianchun Jiang Guoqiang Song Yajing Wang Ziheng Zhuang Ying Tan Yan Xia Xianfeng Huang Xiaoqing Feng 《International journal of molecular sciences》2021,22(11)
Urolithins (hydroxylated 6H-benzo[c]chromen-6-ones) are the main bioavailable metabolites of ellagic acid (EA), which was shown to be a cognitive enhancer in the treatment of neurodegenerative diseases. As part of this research, a series of alkoxylated 6H-benzo[c]chromen-6-one derivatives were designed and synthesized. Furthermore, their biological activities were evaluated as potential PDE2 inhibitors, and the alkoxylated 6H-benzo[c]chromen-6-one derivative 1f was found to have the optimal inhibitory potential (IC50: 3.67 ± 0.47 μM). It also exhibited comparable activity in comparison to that of BAY 60-7550 in vitro cell level studies. 相似文献
73.
Fiber-based hygroresponsive torsional actuators provide desirable merits, such as light weight and shapeability, for developing smart systems to harvest energy from moisture which is a ubiquitous natural resource. A key challenge in this development is to realize moisture-triggered actuation combining large actuation and rapid responses. Here, a multiscale design strategy is explored to create high-performance hygroresponsive torsional actuators consisting of chitosan and multiwalled carbon nanotubes (MWCNTs). The superior actuation performance arises from the synergism of contributing factors at different scales, including 1) MWNCTs accelerate the water transport in primary twisted fibers (PTFs), fostering the rotation of PTFs upon moisture stimuli; 2) in situ-formed hierarchically-assembled twists realize cascade amplification of moisture-triggered actuation. Specifically, PTFs are self-twisted to generate secondary helical yarns, that are subsequently over-twisted to yield tertiary coiled yarn. The resultant yarn actuator can reach a maximum rotation speed of 11 400 rpm in 5 s, output gravitational potential energy of 2.4 J kg−1 and gravitational potential power of 0.053 W kg−1 during contraction. This work represents the first design of fiber-based actuators by virtue of moisture-triggered in situ formation of yarns. The established principles of multiscale design will enable high-performance fiber-based hygroresponsive actuators toward advanced intelligent textile and soft robotics. 相似文献
74.
75.
随着飞行器飞行速度和发动机燃烧室温度的提高, 金属类隐身涂层材料已无法满足环境使用要求。 CeO2
具有较高熔点、 抗氧化、 较低红外发射率等特点, 被认为是红外隐身应用的候选材料, 但其发射率仍无法满足
高温红外隐身需求。 本文通过固相反应法对 CeO2 进行 La3+ 掺杂改性, 研究掺杂量对 CeO2 粉体红外发射性能的
影响规律。 结果表明, 随着掺杂量从 10 mol% 增加到 30 mol%, 粉体在 3~6 μm 波段的发射率不断降低, 其中
Ce0.7La0.3O2 的红外发射率仅为 0.1。 相似文献
76.
选用微型齿轮结构作为研究对象,在使用MoldFlow进行模拟分析的基础上.对微型齿轮注射成型进行试验研究。 相似文献
77.
Effects of Austempering after Hot Deformation on the Mechanical Properties of Hot Rolled Si-Mn TRIP Steel Sheets 总被引:1,自引:0,他引:1
LIZhuang ZHANGPing-li WUDi 《材料热处理学报》2004,25(5):248-251
Excellent mechanical properties are obtained by austempering after hot deformation without subsequent heat treatment in the present Si-Mn TRIP steel sheets. Isothermal holding time after finishing rolling has affected the mechanical properties of this steel. The results show that the sample exhibits a good combination of ultimate tensile strength and total elongation when it is held at the bainite transformation temperature after hot deformation. The stability of retained austenite increases with an increase of isothermal holding time, and a further increase in the holding duration results in a decrease of it. The tensile strength, total elongation and strength ductility reach the maximum values (774MPa, 33% and 25542MPa% respectively) for this sort of hot rolled Si-Mn TRIP steel using the optimal technology. 相似文献
78.
79.
等离子喷涂纳米Al2O3-13%TiO2涂层组织与性能 总被引:2,自引:0,他引:2
采用大气等离子喷涂方法制备了纳米结构Al2O3-13%TiO2(质量分数,下同)涂层,进行了SRV滑动磨损试验,通过扫描电镜对涂层的断口显微组织及磨损表面形貌进行了观察,并测定了涂层的显微硬度.结果表明,该纳米涂层具有独特的显微组织结构特征,层片间具有冶金结合特征的界面较多,界面结合得到明显改善,其显微硬度明显高于微米涂层,具有优良的耐磨性能,其磨损面积仅为常规微米涂层的1/5,界面结合改善是耐磨性提高的主要原因. 相似文献
80.
高温高压设备瞬态密封分析的三维力学与传热学模型 总被引:2,自引:0,他引:2
以大型加氢反应器的高压法兰密封结构为研究对象,建立了瞬态及稳态密封分析的三维力学与传热学模型,主要解决了螺栓载荷的动态模拟和螺栓孔内空气环层的传热计算问题。利用该模型对加氢反应器的瞬态及稳态密封状况进行了计算。 相似文献