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81.
82.
Filippo Favretto Dr. Michael Assfalg Dr. Mariana Gallo Prof. Daniel Oscar Cicero Dr. Mariapina D'Onofrio Prof. Henriette Molinari 《Chembiochem : a European journal of chemical biology》2013,14(14):1807-1819
Human liver fatty acid binding protein (hL‐FABP) has been reported to act as an intracellular shuttle of lipid molecules, thus playing a central role in systemic metabolic homeostasis. The involvement of hL‐FABP in the transport of bile salts has been postulated but scarcely investigated. Here we describe a thorough NMR investigation of glycocholate (GCA) binding to hL‐FABP. The protein molecule bound a single molecule of GCA, in contrast to the 1:2 stoichiometry observed with fatty acids. GCA was found to occupy the large internal cavity of hL‐FABP, without requiring major conformational rearrangement of the protein backbone; rather, this led to increased stability, similar to that estimated for the hL‐FABP:oleate complex. Fast‐timescale dynamics appeared not to be significantly perturbed in the presence of ligands. Slow motions (unlike for other proteins of the family) were retained or enhanced upon binding, consistent with a requirement for structural plasticity for promiscuous recognition. 相似文献
83.
Improvement on Sliding Wear Behavior of Al/Cast Iron Tribopair by CNT's Reinforcement of an Al Alloy
Oscar Carvalho Mihaela Buciumeanu Delfin Soares Jose Gomes Filipe S. Silva 《摩擦学汇刊》2013,56(4):643-653
The results presented in this work show the wear characterization of Al-Si matrix composites reinforced by multiwall carbon nanotubes (MWCNTs) under dry reciprocating sliding conditions against a grey cast iron (GCI) The wear resistance is investigated as a function of the carbon nanotube (CNT) content that varied from 2 to 6 wt%. The results demonstrated that the CNT content plays a relevant role in the wear behavior by substantially reducing the wear loss of Al-Si CNT composites. Further, it reduces the wear loss of the grey cast iron counterface. A physical model able to explain the improved behavior in both mating materials is depicted from experimental results. 相似文献
84.
One basic principle of fluid mechanics used to resolve practical problems in hydraulic engineering is the Bernoulli theorem along a streamline, deduced from the work-energy form of the Euler equation along a streamline. Some confusion exists about the applicability of the Bernoulli theorem and its generalization to open-channel hydraulics. In the present work, a detailed analysis of the Bernoulli theorem and its extension to flow in open channels are developed. The generalized depth-averaged Bernoulli theorem is proposed and it has been proved that the depth-averaged specific energy reaches a minimum in converging accelerating free surface flow over weirs and flumes. Further, in general, a channel control with minimum specific energy in curvilinear flow is not isolated from water waves, as customary state in open-channel hydraulics. 相似文献
85.
86.
This paper considers the design of a software sensor (or soft-sensor) for the on-line estimation of the biological activities of a colony of aerobic micro-organisms acting on activated sludge processes, where the carbonaceous waste degradation and nitrification processes are taken into account. These bioactivities are intimately related to the dissolved oxygen concentration. Two factors that affect the dynamics of the dissolved oxygen are the respiration rate or the oxygen uptake rate (OUR) and the oxygen transfer function (K(l)a). These items are challenging topics for the application of recursive identification due the nonlinear characteristic of the oxygen transfer function, and to the time-varying feature of the respiration rate. In this work, OUR and the oxygen transfer function are estimated through a software sensor, which is based on a modified version of the discrete extended Kalman filter. Numerical simulations are carried out in a predenitrifying activated sludge process benchmark and the obtained results demonstrate the applicability and efficiency of the proposed methodology, which should provide a valuable tool to supervise and control activated sludge processes. 相似文献
87.
We have developed a method for calibrating subnanometer movements of a piezoelectric actuator with picometer accuracy and for a wide range of frequencies. This range make this calibration useful for scanning probe microscopes, particularly for an apertureless scanning near-field optical microscope in which the tip is dithered to modulate the optical signal. The setup consists of a Michelson interferometer that has a mobile arm capable of moving more than one fringe. The piezoelectric actuator to be calibrated vibrates at the desired frequency in the other arm. Net displacement can be calculated by simultaneous measurement of an interferometric signal and its derivative. Hysteresis of the system can be also measured. It will be shown that the actuator response is linear only for the low-frequency region (in our case as much as approximately 10 kHz). Above that frequency range, higher harmonics appear and cannot be neglected to obtain real displacement. Finally, it will be shown that the use of higher harmonics in calibration or detection schemes (that rely on the linearity of the response) must be validated, and this technique has proved adequate for that purpose. 相似文献
88.
Castillo E Fontenla-Romero O Guijarro-Berdiñas B Alonso-Betanzos A 《Neural computation》2002,14(6):1429-1449
The article presents a method for learning the weights in one-layer feedforward neural networks minimizing either the sum of squared errors or the maximum absolute error, measured in the input scale. This leads to the existence of a global optimum that can be easily obtained solving linear systems of equations or linear programming problems, using much less computational power than the one associated with the standard methods. Another version of the method allows computing a large set of estimates for the weights, providing robust, mean or median, estimates for them, and the associated standard errors, which give a good measure for the quality of the fit. Later, the standard one-layer neural network algorithms are improved by learning the neural functions instead of assuming them known. A set of examples of applications is used to illustrate the methods. Finally, a comparison with other high-performance learning algorithms shows that the proposed methods are at least 10 times faster than the fastest standard algorithm used in the comparison. 相似文献
89.
Diego Gutierrez Francisco J. Seron Adolfo Munoz Oscar Anson 《Computers & Graphics》2006,30(6):994-1010
This paper presents a physically based simulation of atmospheric phenomena. It takes into account the physics of non-homogeneous media in which the index of refraction varies continuously, creating curved light paths. As opposed to previous research on this area, we solve the physically based differential equation that describes the trajectory of light. We develop an accurate expression of the index of refraction in the atmosphere as a function of wavelength, based on real measured data. We also describe our atmosphere profile manager, which lets us mimic the initial conditions of real-world scenes for our simulations. The method is validated both visually (by comparing the images with the real pictures) and numerically (with the extensive literature from other areas of research such as optics or meteorology). The phenomena simulated include the inferior and superior mirages, the Fata Morgana, the Novaya–Zemlya, the Viking's end of the world, the distortions caused by heat waves and the green flash. 相似文献
90.
O. H. Willemsen S. T. De Zwart M. G. H. Hiddink Oscar Willemsen 《Journal of the Society for Information Display》2006,14(8):715-722
Abstract— In this paper, the design of a lenticular‐based 2‐D/3‐D display for mobile applications is described. This display combines look‐around capability with good 3‐D resolution. In order to allow high‐resolution datagraphic applications, a concept based on actively switched lenses has been developed. A very noticeable problem for such displays is the occurrence of dark bands. Despite slanting the lenticular and defocusing the lens, banding becomes unacceptable when the display is viewed from an angle. As a solution, fractional viewing systems to reduce the banding intensity by almost two orders of magnitude is introduced. The resulting 3‐D display can be viewed from any horizontal direction without banding. 相似文献