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排序方式: 共有417条查询结果,搜索用时 15 毫秒
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F. Cynthia Martin Monika Hiller Pietro Spitali Stijn Oonk Hans Dalebout Magnus Palmblad Amina Chaouch Michela Guglieri Volker Straub Hanns Lochmüller Erik H. Niks Jan J. G. M. Verschuuren Annemieke Aartsma-Rus André M. Deelder Yuri E. M. van der Burgt Peter A. C. 't Hoen 《Proteomics. Clinical applications》2014,8(3-4):269-278
64.
Melatonin prevents mitochondrial dysfunctions and death in differentiated skeletal muscle cells 下载免费PDF全文
Sara Salucci Michela Battistelli Valentina Baldassarri Debora Burini Elisabetta Falcieri Sabrina Burattini 《Microscopy research and technique》2017,80(11):1174-1181
Oxidative stress increase induces cellular damage and apoptosis activation, a mechanism believed to represent a final common pathway correlated to sarcopenia and many skeletal muscle disorders. The goal of this study is to evaluate if melatonin, a ROS scavenger molecule, is able to counteract or modulate myotube death. Here, differentiated C2C12 skeletal muscle cells have been treated with melatonin before chemicals known to induce apoptotic death and oxidative stress, and its effect has been investigated by means of morpho‐functional analyses. Ultrastructural observations show melatonin protection against triggers by the reducing of membrane blebbing, chromatin condensation, myonuclei loss and in situ DNA cleavage. Moreover, melatonin is able to prevent mitochondrial dysfunctions which occur in myotubes exposed to the trigger alone. These findings demonstrate melatonin ability in preventing apoptotic cell death in skeletal muscle fibers in vitro, suggesting for this molecule a potential therapeutic role in the treatment of various muscle disorders. 相似文献
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M. Luisa Demattè Gesualdo M. Zucco Sergio Roncato Paola Gatto Elena Paulon Raffaele Cavalli Michela Zanetti 《Holz als Roh- und Werkstoff》2018,76(4):1093-1100
The effect a wooden-built environment exerts on the physical and psychological well-being of people has mostly been investigated by evaluation, through a limited number of sensory modalities, of small size stimuli. In this research, two real-size wooden and plaster indoor settings were used to examine the influence of wood on people’s emotions and perceptions. Three questionnaires assessed participants’ multimodal sensory, affective, and cognitive responses to the settings (Semantic Differential questionnaire), their emotional state (Positive and Negative Affect Schedule), and their environmental behaviour and biophilia degree (Nature Relatedness Scale). The results revealed that wood induces more positive emotions than plaster, and that individual biophilia degree appears to influence the tactile, auditory, and olfactory evaluation of the settings. It furthermore indicates how wood used in real buildings positively influences human psychological well-being and highlights the importance of considering multiple sensory modalities when investigating wood–human interaction. 相似文献
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Wörner M Lioubashevski O Basel MT Niebler S Gogritchiani E Egner N Heinz C Hoferer J Cipolloni M Janik K Katz E Braun AM Willner I Niederweis M Bossmann SH 《Small (Weinheim an der Bergstrasse, Germany)》2007,3(6):1084-1097
Nanostructures with long-term stability at the surface of gold electrodes are generated by reconstituting the porin MspA from Mycobacterium smegmatis into a specially designed monolayer of long-chain lipid surfactant on gold. Tailored surface coverage of gold electrodes with long-chain surfactants is achieved by electrochemically assisted deposition of organic thiosulfates (Bunte salts). The subsequent reconstitution of the octameric-pore MspA is guided by its extraordinary self-assembling properties. Importantly, electrochemical reduction of copper(II) yields copper nanoparticles within the MspA nanopores. Electrochemical impedance spectroscopy, reflection electron microscopy, and atomic force microscopy (AFM) show that: 1) the MspA pores within the self-assembled monolayer (SAM) are monodisperse and electrochemically active, 2) MspA reconstitutes in SAMs and with a 10-nm thickness, 3) AFM is a suitable method to detect pores within SAMs, and 4) the electrochemical reduction of Cu2+ to Cu0 under overpotential conditions starts within the MspA pores. 相似文献
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Michela Pecoraro Stefania Marzocco Ada Popolo 《International journal of molecular sciences》2021,22(21)
Hypoxia is the leading cause of death in cardiomyocytes. Cells respond to oxygen deprivation by activating cytoprotective programs, such as mitochondrial connexin43 (mCx43) overexpression and the opening of mitochondrial KATP channels, aimed to reduce mitochondrial dysfunction. In this study we used an in vitro model of CoCl2-induced hypoxia to demonstrate that mCx43 and KATP channels cooperate to induce cytoprotection. CoCl2 administration induces apoptosis in H9c2 cells by increasing mitochondrial ROS production, intracellular and mitochondrial calcium overload and by inducing mitochondrial membrane depolarization. Diazoxide, an opener of KATP channels, reduces all these deleterious effects of CoCl2 only in the presence of mCx43. In fact, our results demonstrate that in the presence of radicicol, an inhibitor of Cx43 translocation to mitochondria, the cytoprotective effects of diazoxide disappear. In conclusion, these data confirm that there exists a close functional link between mCx43 and KATP channels. 相似文献
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Viviana De Martino Michela Rossi Giulia Battafarano Jessica Pepe Salvatore Minisola Andrea Del Fattore 《International journal of molecular sciences》2021,22(22)
Osteosarcoma (OS) is a skeletal tumor affecting mainly children and adolescents. The presence of distance metastasis is frequent and it is localized preferentially to the lung, representing the main reason for death among patients. The therapeutic approaches are based on surgery and chemotherapeutics. However, the drug resistance and the side effects associated with the chemotherapy require the identification of new therapeutic approaches. The understanding of the complex biological scenario of the osteosarcoma will open the way for the identification of new targets for its treatment. Recently, a great interest of scientific community is for extracellular vesicles (EVs), that are released in the tumor microenvironment and are important regulators of tumor proliferation and the metastatic process. At the same time, circulating extracellular vesicles can be exploited as diagnostic and prognostic biomarkers, and they can be loaded with drugs as a new therapeutic approach for osteosarcoma patients. Thus, the characterization of OS-related EVs could represent a way to convert these vesicles from antagonists for human health into therapeutic and/or diagnostic agents. 相似文献
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Ruffolo Silvestro A. La Russa Mauro F. Ricca Michela Belfiore Cristina M. Macchia Andrea Comite Valeria Pezzino Antonino Crisci Gino M. 《Bulletin of Engineering Geology and the Environment》2017,76(1):11-20
Bulletin of Engineering Geology and the Environment - The deterioration of a stone material is related to its pore structure, which affects the interaction between surface and environmental agents.... 相似文献