Functionalization of single- and multi-walled carbon nanotubes with cationic amphiphiles for plasmid DNA complexation and transfection

The possibility of delivering DNA efficiently to cells represents a crucial issue for the treatment of both genetic and acquired diseases. However, even although the efficiency of non-viral transfection systems has improved in the last decade, none have yet proven to be sufficiently effective in vivo. We report herein our results on the functionalization of single-walled carbon nanotubes (SWNT) and multi-walled carbon nanotubes (MWNT) by two cationic amphiphiles (lipid RPR120535 and pyrenyl polyamine), their use for the complexation of plasmid DNA, and their efficiency in transfecting cells in vitro. The experiments have shown that the efficiency of transfection is higher when using SWNT instead of MWNT, and that transfection efficiency is similar or slightly higher when using nanoplexes (SWNT/lipid RPR120535/DNA) instead of lipoplexes (lipid RPR120535/DNA) and several orders of magnitude higher than that of naked DNA. This study therefore shows both that the transfection is better when using SWNTs and that it is dependent on the nature of the amphiphilic molecules adsorbed on the nanotubes.      

Application of the orthogonal collocation method to determination of temperature distribution in cylindrical conductors

In this work, an analytical model for the determination of the temperature distribution in cylindrical heater components with characteristics of nuclear fuel rods, is presented. The heat conductor is characterized by an arbitrary number of solid walls and different types of materials, whose thermal properties are taken as function of temperature. The heat conduction fundamental equation is solved numerically with the method of weighted residuals (MWR) using a technique of orthogonal collocation. The results obtained with the proposed method are compared with the experimental data from tests performed in the TRIGA IPR-R1 research reactor localized at the CDTN/CNEN (Centro de Desenvolvimento da Tecnologia Nuclear/Comissão Nacional de Energia Nuclear) at Belo Horizonte in Brazil.     

Coupled visco-mechanical and diffusion void growth modelling during composite curing

Most critical processing step during long fiber reinforced epoxy matrix composite laminate manufacturing is the polymerization stage. If not optimized, it gives birth to defects in the bulk material, such as voids. These defects are considered as possible sources of damage in the composite parts. The aim of this work is to model the evolution of void growth in thermoset composite laminates after ply collation (autoclave processes) or resin impregnation (RTM, LCM process). A coupled mechanical and diffusion model is presented to better predict the final void size at the end of polymerization. Amongst the parameter investigated, onset of pressure application and diffusive species concentration where found to have a major effect on void size evolution during curing process.     

Multi-laboratory simulator studies on effects of serum proteins on PTFE cup wear

A multi-laboratory, simulator study investigated the wear of polytetrafluorethylene (PTFE) cups run in bovine serum. Each laboratory used its own test protocol with a variety of simulator types. Our wear model incorporated 32 mm dia CoCr heads matched to PTFE cups run with serum protein-concentrations in the range 17–69 mg/ml. The multi-lab data demonstrated that protein-concentration had the most significant effect on wear performance. Both inverted and anatomical cups followed the same trend with first a rapid increase in wear-rates apparent for the initially low-protein levels and then a wear-rate reduction effect becoming apparent beyond 17 mg/ml of proteins. The results showed that as the protein concentration increased from 17 to 69 mg/ml, the magnitude of the wear-rates increased 200% but the protein wear-rate gradient decreased 24–60% with “inverted” and “anatomical” cups, respectively. This effect was more pronounced with ‘anatomical” than “inverted” cups. Thus, the wear-trends with “inverted” cups were generally the more consistent, particularly at the low-protein levels. Increasing the serum volume by two-fold in one study increased the PTFE wear-magnitudes approximately 40% and the protein-wear gradient by 30%. These PTFE wear phenomena were consistent with the concept that low-concentrations of proteins promoted polymer wear but high-protein concentrations resulted in a protein-degradation phenomenon which progressively masked the actual polymer wear. In the selected protein range 17–69 mg/l, the multi-laboratory simulator data consistently overestimated the average clinical wear-rate by at least 50–100% depending on protein range. It would, therefore, appear clinically relevant to study PTFE wear with an inverted-cup model using a large volume of serum but only in low-protein concentrations. The protein-related wear phenomena observed with PTFE cups in this multi-laboratory project may also have relevance for wear-simulation of UHMWPE cups.     

Weighted proportional window control of TCP traffic

This article describes a technique for weighted proportional window control of elastic traffic such as that generated by TCP. This is achieved through the modification of the receiver's advertised window of TCP connections sharing the bottleneck link while taking into account the price that each user of a connection has paid for the service and the total number of active connections sharing the bottleneck link. Copyright © 2001 John Wiley & Sons, Ltd.     

Numerical simulation of non-perforating impacts on shielded gas-filled pressure vessels

In order to calibrate the output of hydrocode simulations of hypervelocity impacts on shielded gas-filled pressure vessels, Light Gas Gun impact experiments were performed. In a first step, tests were performed on so-called equivalent Whipple shield (EWS) configurations having basically the same set-up as the shielded pressure vessels (i.e. bumper thickness and - material, stand-off and backwall plate thickness and -material). Purpose was the determination of the impact conditions that lead to penetration into the backwall plate but not perforation of it or leakage through the impacted area. In a second step, impact tests on the corresponding shielded pressure vessels were performed with the same test conditions as the EWS. The purpose of the tests was the investigation whether leakage occurs when the vessel's front wall is not perforated, but just cratered. The test conditions lead to no leakage in all tests. The most important measured damage parameter was the crater depth of the deepest crater in the vessel's front wall/the backwall plate of the EWS, respectively. Hydrocode simulations were then performed to assess the capability of the numerical tool to correctly predict the damage on the impacted vessel surface. Normal impacts of aluminium spheres against shielded vessels were simulated using AUTODYN-2D, including and evaluating the effect of the static stress induced in the vessel walls by the inner pressure. Particular attention was focused on the exact determination of the maximum crater depth caused by the debris cloud impact on the vessel wall/the backwall plate of the EWS, respectively. Bumper and projectile were represented by SPH particles, the vessel shell was represented by a Lagrange grid. The results showed a very good agreement with the measured crater depths of the experiments.     

VIRCON: Interactive System for Teaching Construction Management

The focus in this paper is on a system, developed by the writers, called VIRCON (short for VIRtual CONstruction), in which the traditional construction planning is combined with 3D∕4D models of the project. To facilitate current best practices with 3D∕4D models of the project, VIRCON has been implemented using object-oriented programming, client/server configuration, database management information, and CAD systems. The real innovation in the design of VIRCON is associated with the unique scheduling and simulation engine developed to integrate cost planning and scheduling and accommodate integrated cross-impact analysis. VIRCON has been validated by means of student group projects on a course where many of the project management techniques are being taught. The teaching approach conducted with the utilization of VIRCON has shown the way forward in creating a dynamic and interactive learning atmosphere. This paper also outlines the experience gained from teaching construction planning fundamentals by means of the VIRCON system.     

Creep crack initiation and creep crack growth assessments in welded structures

In this study, creep crack initiation and creep crack growth in welded structure are analysed. An interaction phenomenon between base metal and weld metal in a cross weld plate is highlighted with a finite element model. A simplified method based on the reference stress approach is proposed to evaluate C* in welded structures. This simplified method is applied for creep crack initiation and creep crack growth assessments in the case of a double edge notched tension cross-welded plate. Correlations between crack initiation time and C* on the one hand, and between creep crack growth rate and C* on the other hand are used. Creep crack initiation time estimation for the full size welded plate is very conservative when crack initiation properties of CT specimen are used. Concerning creep crack growth evaluation, simplified estimation is in good agreement with experimental results when CT specimen crack propagation properties are used.