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101.
A Kato T Kitamura C Sugimoto Y Ogawa K Nakazato K Nagashima WW Hall K Kawabe Y Yogo 《Canadian Metallurgical Quarterly》1997,142(5):875-882
Human polyomavirus JC virus (JCV), the causative agent of progressive multifocal leukoencephalopathy, is ubiquitous in humans, infecting children asymptomatically then persisting in renal tissue. Since JCV DNA can readily be detected from urine, it should be a useful tool with which to study the mode of virus transmission in humans. Based on this notion, we examined the extent to which JCV was transmitted from the American to Japanese populations in Okinawa Island, Japan. (A population of about 50 000 American soldiers and families have been stationed in Okinawa since 1945.) Four JCV types (A to D) were identified in American populations in U.S.A., whereas only type B was prevalent in elder Japanese in Okinawa who had reached adulthood by 1945. Thus, types A, C, and D served as indicators of the transmission of JCV from American to Japanese populations. We then examined whether types A, C, and D were detectable in Japanese in Okinawa aged 30-50 years who may have been in contact with Americans during childhood. However, all the 125 isolates from the younger Japanese population were type B without exception. From this finding, we concluded that JCV is rarely transmitted between human populations. 相似文献
102.
Tomoyuki Kawaguchi Yasuhiro Rachi Wataru Sugimoto Yasushi Murakami Yoshio Takasu 《Journal of Applied Electrochemistry》2006,36(10):1117-1125
Highly dispersed ternary PtRuRh/C anode catalysts for direct methanol fuel cells were prepared with various contents and their electro-catalytic activities towards methanol oxidation at 25 °C and 60 °C were examined to investigate the influence of the catalyst composition. Electrocatalysts were prepared by a co-impregnation method using ethanolic solutions of metal precursors and carbon black followed by pyrolysis under reducing conditions. X-ray diffraction analysis revealed that the fcc peaks shifted to higher diffraction angles with increasing Rh content, indicating the alloying of Rh into the fcc structure. In terms of the mass specific current density, the activity towards methanol oxidation differed significantly depending on the catalysts composition and cell temperature. The catalyst prepared at a ratio of Pt:Ru:Rh = 1:1:2 exhibited the highest activity at 60 °C of 155 A (g-Pt)−1 at 0.5 V vs. RHE. 相似文献
103.
A Myoumoto N Manabe Y Imai Y Kimura M Sugimoto Y Okamura M Fukumoto K Sakamaki Y Niwano H Miyamoto 《Canadian Metallurgical Quarterly》1997,59(8):641-649
Two monoclonal antibodies capable of inducing granulosa cell apoptosis were produced against granulosa cells prepared from antral follicles of pig ovaries. The healthy follicles, 4-5 mm in diameter, were dissected from the ovaries of gilts, and then granulosa cells were isolated. BALB/c female mice were immunized with the isolated granulosa cells. Antibodies against the granulosa cells were detected by immunofluorescent staining using frozen ovarian sections. The isolated spleen cells prepared from immunized mice producing antibodies against the granulosa cells were fused with Sp2/O-Ag 14 mouse myeloma cells by standard hybridization techniques. Two hybridoma clones, PFG-1 and PFG-2, which produced specific IgM antibodies against granulosa cells were selected. Western blotting analysis revealed that PFG-1 and PFG-2 antibodies specifically recognized cell-membrane proteins with molecular weights of 55 and 70 kD and isoelectric points of 5.9 and 5.4, respectively. The monoclonal antibodies immunohistochemically reacted with granulosa cells of healthy follicles. When the isolated granulosa cells prepared from healthy follicles were cultured in medium containing 0.1 or 10 micrograms/m/PFG-1 or PFG-2 antibodies, respectively, the cells underwent apoptosis as determined by nuclear morphology, DNA electrophoresis and flow cytometric analysis. In conclusion, these two monoclonal antibodies against granulosa cells have cell-killing activity in cultured granulosa cells. 相似文献
104.
Y Abe S Umemura K Sugimoto N Hirawa Y Kato N Yokoyama T Yokoyama J Iwai M Ishii 《Canadian Metallurgical Quarterly》1995,8(1):74-79
A method of reducing the amount of data required to reconstruct an image is described. In this scheme, fully sampled low spatial frequency data are acquired up to a given cutoff frequency and above this point, only alternate lines are sampled. Two images are produced, one of low definition and one of high definition but aliased. The proposed algorithm unwraps the aliased data, which are then used to enhance the low pass image, yielding a best estimate of the true image. The reduced sampling technique is shown to afford biological images that are almost indistinguishable from those obtained from a complete data set. 相似文献
105.
The characteristics of control system design using a universal learning network (ULN) are such that both the controlled systems
and their controller are represented in a unified framework, and that the learning stage of the ULN can be executed by using
not only first-order derivatives (gradient) but also the higher order derivatives of the criterion function with respect to
parameters. ULNs have the same generalization ability as neural networks. So the ULN controller is able to control the system
in a favorable way under an environment which is little different from the environment of the control system at the learning
stage. However, stability cannot be sufficiently realized. In this paper, we propose a robust control method using a ULN and
second-order derivatives of that ULN. Robust control, as considered here, is defined as follows. Even though the initial values
of the node outputs are very different from those at the learning stage, the control system is able to reduce its influence
to other node outputs and can control the system as in the case of no variation. In order to realize such robust control,
a new term concerning the variation is added to the usual criterion function, and the parameters are adjusted so as to minimize
the above-mentioned criterion function using second-order derivatives of the criterion function with respect to the parameters.
Finally, it is shown that the ULN controller constructed by the proposed method works effectively in a simulation study of
a non-linear crane system.
This work was presented, in part, at the International Symposium on Artificial Life and Robotics, Oita, Japan, February 18–20,
1996 相似文献
106.
This paper demonstrates the low-voltage and low-power operation of a MOS sample-and-hold circuit while preserving speed and accuracy, aiming at the realization of a pipelined low-voltage and low-power analog-to-digital converter on a system large-scale integrated circuit. It was fabricated by utilizing 0.35-/spl mu/m CMOS technology. The main feature of this circuit is that all the input, signals, and output are in the current form. The circuit consists of simple current mirrors. In order to eliminate the signal-dependent current transfer ratio error, voltages at the drain terminals of mirror transistors are fixed as constant. A source degeneration resistor, which is a transistor in the triode operational region, is connected to a mirror transistor in order to alleviate the influence of the threshold and transconductance parameter variations. Control signals are boosted in voltage and applied to the gate of switch NMOS transistors in the signal path in order to reduce the on-resistance of analog switches. A differential configuration is adopted throughout the entire circuit and effectively cancels switch feedthrough errors. As a result, a 30-MS/s operation with a signal-to-noise ratio (SNR) of 56 dB from a 1-V supply has been achieved, when the input current is /spl plusmn/200 /spl mu/A. The chip even operated down to 0.85 V with a 20-MHz clock. The SNR was measured as 50 dB with an input current of /spl plusmn/100 /spl mu/A. 相似文献
107.
Effective acid sites for the dehydration of 2-propanol and isomerization of 1-butene were found to be generated by glass formation of calcium metaphosphate. The number of these sites with strong acidity remarkably decreased by the crystallization of the glass, where the condensation of hydroxyl groups were significant. Thus, the sites must be the monohydrogen phosphate protons. 相似文献
108.
The inhibitory effects of a potent nitric oxide (NO) synthase inhibitor, N(G)-nitro-L-arginine methyl ester (L-NAME), on 2-deoxy-D-glucose (2-DG)-induced hyperglycemia were investigated in rats. L-NAME significantly inhibited 2-DG-induced hyperglycemia, although N(G)-nitro-D-arginine methyl ester (D-NAME) did not affect it. A similar NO synthase inhibitor, N(G)-monomethyl-L-arginine (L-NMMA), also inhibited 2-DG-induced hyperglycemia. The antagonistic effects of L-NAME are unrelated to the cholinergic system, since the muscarinic receptor antagonist scopolamine did not affect 2-DG-induced hyperglycemia. The neuronal NO synthase inhibitor 7-nitroindazole (7-NI) did not reduce 2-DG-induced hyperglycemia, but rather enhanced it. Our results suggest that NO may be involved in glucose homeostasis and that the inhibitory effects of L-NAME on 2-DG-induced hyperglycemia are not related to muscarinic receptors or neuronal NO synthase. 相似文献
109.
Resistivity measurements on the hydrogenation disproportionation desorption recombination (HDDR) phenomena in near stoichiometric Nd---Fe---B alloys were carried out in the temperature range of T=740–900 °C in order to investigate the effect of additional elements such as Co, Ga and Zr. These studies were combined with detailed temperature-pressure-analysis to relate the observed changes in resistivity with the solubility of hydrogen in the rare-earth metal. Higher relative resistivity changes can be observed when lower disproportionation temperatures are applied. This tendency depends on the amount of hydrogen absorbed in the disproportionated mixture. The addition of Co leads to a longer completion time of the disproportionation reaction. In particular, the alloy with both Co and Ga additions exhibits the longest completion time. These observations suggest that Co stabilises the 2-14-1 phase and Ga addition enhances this effect. These additional elements decrease the number of hydrogen atoms absorbed per formula unit and also increase the onset hydrogen pressure of the disproportionation and recombination reactions. On desorption, Co and Ga additions decrease the activation energy of the recombination reaction and lead to a higher reaction rate of these alloys compared to that of the ternary alloy, which is especially pronounced during the initial stages of recombination and which may be relevant for the development of anisotropic features in the recombined state. 相似文献
110.
Tsugaru K. Sugimoto Y. Noda M. Ito T. Suwa Y. 《Solid-State Circuits, IEEE Journal of》1990,25(3):653-659
A +5-V single-power-supply 10-b video BiCMOS sample-and-hold IC is described. Video speed, low power, and 10-b accuracy sample-and-hold operation have been achieved using a complementary connected buffer format sample switch. A high-speed p-n-p transistor used in the sample switch is formed by a combination of n-p-n and PMOS transistors. The sample-and-hold operation is accomplished by feeding back the hold capacitor voltage to the sample switch inputs, so that the inputs transfer symmetrically for the hold capacitor voltage at any input level. The sample-and-hold IC has been implemented in 1.2-μm BiCMOS technology and evaluated. The following results have been obtained: 185-MHz 3-dB bandwidth at 22-pF hold capacitor, 63-dB signal-to-noise ratio at 8-MHz full-scale input, 20-ns acquisition time at 1-V step input, 15-ns switch setting time, and 0.1% linearity error. Power dissipation is 150 mW 相似文献