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11.
Kojima Shuichi; Takagi Nobuyuki; Minagawa Tetsuya; Fushimi Noriko; Miura Kin-ichiro 《Protein engineering, design & selection : PEDS》1999,12(10):857-862
We have previously shown that replacing the P1-site residue(Ala) of chicken ovomucoid domain 3 (OMCHI3) with a Met or Lysresults in the acquisition of inhibitory activity toward chymotrypsinor trypsin, respectively. However, the inhibitory activitiesthus induced are not strong. In the present study, we introducedadditional amino acid replacements around the reactive siteto try to make the P1-site mutants more effective inhibitorsof chymotrypsin or trypsin. The amino acid replacement AspTyrat the P2' site of OMCHI3(P1Met) resulted in conversion to a35000-fold more effective inhibitor of chymotrypsin with aninhibitor constant (Ki) of 1.17x1011 M. The Ki valueof OMCHI3(P1Met, P2'Ala) indicated that the effect on the interactionwith chymotrypsin of removing a negative charge from the P2'site was greater than that of introducing an aromatic ring.Similarly, enhanced inhibition of trypsin was observed whenthe AspTyr replacement was introduced into the P2' site of OMCHI3(P1Lys).Two additional replacements, AspAla at the P4 site and ArgAlaat the P3' site, made the mutant a more effective inhibitorof trypsin with a Ki value of 1.44x109 M. By contrast,ArgAla replacement at the P3' site of OMCHI3(P1Met, P2'Tyr)resulted in a greatly reduced inhibition of chymotrypsin, andAspAla replacement at the P4 site produced only a small changewhen compared with a natural variant of OMCHI3. These resultsclearly indicate that not only the P1-site residue but alsothe characteristics, particularly the electrostatic properties,of the amino acid residues around the reactive site of the proteaseinhibitor determine the strength of its interactions with proteases.Furthermore, amino acids with different characteristics arerequired around the reactive site for strong inhibition of chymotrypsinand trypsin. 相似文献
12.
Keiji Kobayashi 《Journal of the American Ceramic Society》1990,73(11):3500-3502
F− - and OH− -free ZnO-B2 O3 -SiO2 -Al2 O3 -P2 O5 glasses used for semiconductor-device passivation or insulation are investigated with regard to compositional dependencies for thermal expansion, viscosity points, and metal oxide semiconductor (MOS) capacitor properties. The experimental data show that thermal expansion increases, and flow points decrease, when P2 O5 is substituted for B2 O3 . MOS capacitors passivated by OH− - and F− -free ZnO-based glasses exhibit normal capacitance-voltage curves. 相似文献
13.
Keiji Hayashi Tomoki Shiraishi Kazuho Toyoda Fumiya Tanaka Taiga Mori Takumi Hata 《Computer Physics Communications》2011,(9):2032-2035
Laws of dynamic nano-friction (i.e., continuous wearless friction) were searched for under steady spatial distributions of the local quasi-temperature, by molecular dynamics (MD) simulations. The temperature control of the non-conservative model was carried out by extending the isothermal MD method using the Nosé–Poincaré thermostat. The results suggested that the threshold phenomenon characterizes sliding-velocity dependence of the nano-frictional force between crystal lattices constituting a nano-electromechanical system (NEMS). This phenomenon was turned out to be a universal feature, whether heat transfer to the environment exists or not. 相似文献
14.
Mitsuyoshi Nagao Masahito Yamamoto Keiji Suzuki & Azuma Ohuchi 《International Transactions in Operational Research》2001,8(1):31-45
In this paper, we propose a face identification system based on the Mahalanobis–Taguchi System (MTS). The MTS is one of the pattern recognition methods frequently used in quality engineering, and can perform robust pattern recognition by using training data, including noise. It is likely that this advantage will allow the effective implementation of a robust face identification system against lighting and face position fluctuations. Moreover, the MTS can optimize the number of attributes required for identification by using the orthogonal array and the signal/noise (SN) ratio. The face identification system has to deal with many users and the amount of data in a facial image is large. Therefore, the time required for identification can be decreased and the amount of data in the facial image database can be reduced by performing the optimization. We confirmed the effectiveness of the proposed system through practical experiments. The experimental results revealed that the MTS was an effective method for robust face identification, and could effectively reduce the number of attributes required for identification. 相似文献
15.
Keiji Kamimura Jun'ichiro Muto Kazunori Akiyama 《Journal of Materials Science: Materials in Electronics》1991,2(4):244-247
Electrical conductivity of evaporated zinc phthalocyanine (ZnPc) films, doped with orthochloranil (o-CA) molecules, is investigated. Benzoico-CA solution is introduced in the film and benzene is removed by vapourization afterwards. The film conductivity at first increased and then decreased with incremental doping. The maximum conductivity of the sample appeared at doping level × (=o-CA/ZnPc) 0.2 and is about 103 times as large as that of the as-evaporated one. About a ten-fold further increase in the conductivity is obtained by the heat-treatment of 150 °C for 12 h. The conductivity change by doping is explained by taking into account thato-CA molecules behave not only as acceptors in the film but destructively for the film structure. The phase change of ZnPc by benzoic solution is also discussed. 相似文献
16.
In this study, thermomechanical properties of titanium-nickel (Ti-Ni) shape memory alloy (SMA) films are investigated in order to derive constitutive relations. Ti-Ni SMA films, deposited by DC magnetron sputtering under controlled film composition, are characterized by uniaxial tensile tests. At room temperature (R.T.), Ti-Ni films having Ti contents less than 50 at% exhibit superelastic behavior, and those having Ti contents greater than 50 at% exhibit shape memory behavior. However, the Ni—53.2 at% Ti film fractured at a tensile strain of 0.8% because of an increase in brittleness with increasing Ti content. At elevated temperatures, Ti-Ni films having Ti contents of 50.2 to 52.6 at% undergo phase change from martensite to austenite. The Young's modulus of the Ti-Ni films depends on temperature at each phase, regardless of film composition. Film composition does, however, affect the measured material constants bA, bM, cA, and cM. Stress-strain curves calculated from the constructed constitutive equation closely agree with those obtained from tensile tests, for both the martensite and austenite phases. The constitutive equations are expected to find great utility in the design of Ti-Ni film-actuated microelectromechanical systems (MEMS). 相似文献
17.
Weidong Ding Ryota Hayashi Kohei Ochi Junya Suehiro Kiminobu Imasaka Masanori Hara Noriaki Sano Eiichi Nagao Tadao Minagawa 《Dielectrics and Electrical Insulation, IEEE Transactions on》2006,13(6):1200-1207
Chemical byproducts analysis has been recognized as a powerful diagnosis method for SF6 gas-insulated switchgear (GIS). The authors have previously demonstrated that a carbon nanotube (CNT) gas sensor could detect partial discharge (PD) generated in SF6 gas. However, PD-generated decomposition gas species, which were responsible for the CNT gas sensor response, have not been identified yet. In this paper, two kinds of experiments were conducted in order to identify the responsible decomposition gas species. At first, the decomposition gas molecules adsorbed on CNTs were analyzed by Fourier transformation infrared (FTIR) spectroscopy. FTIR absorbance was observed around 735 cm-1 after CNTs were exposed to PD generated in SF6. In the second experiment, the CNT gas sensor responses to typical SF6 decomposition products (HF and SF4) were examined. The CNT gas sensor responded to these gases in the same way as to PD generated in SF6. SF4 response was larger than HF response. Based on these results, SF 4 and SOF2 emerged as candidates for the responsible decomposition gases. Electrochemical interactions between adsorbed gas molecules and CNT were discussed based on theoretical predictions of molecular orbital calculations. The calculation results suggested that both of SOF2 and SF4 could increase the CNT gas sensor conductance 相似文献
18.
Makoto Ishiguro Katsufumi Ohmuro Yukito Saitoh Yuuta Takahashi Jun Watanabe Tsutomu Arai Yoji Ito Keiji Mihayashi 《Journal of the Society for Information Display》2012,20(11):598-603
We have successfully developed a quarter‐wave retardation film (QWF) for wide viewing angle 3D liquid crystal displays (3D‐LCDs) that provides high luminance, low crosstalk, low color change, and low head‐tilt‐angle dependency. It was found that the out‐of‐plane retardation (Rth) of the QWF in the LCD needs to be close to 0 nm in order to improve the 3D display properties at an off‐axis position and that the in‐plane retardation (Re) needs to be adjusted from 120 to 130 nm to achieve low color change with head tilting. We adopted a coating process for making our QWF because of its potential for retardation control. 3D‐LCDs with this QWF whose Rth was nearly zero had high performance and allowed off‐axis other than on‐axis. 相似文献
19.
Toshihiko Takaya Hidenori Kawamura Yoshihiro Minagawa Masahito Yamamoto Azuma Ohuchi 《Artificial Life and Robotics》2006,10(2):177-184
Blimp robots are attractive as indoor flying robots because they can float in the air, land safely with low energy, and stay
in motion for a long time compared with other flying robots. However, controlling blimp robots is difficult because they have
nonlinear characteristics, are influenced by air streams, and can easily be influenced by inertia. Therefore, a robust and
adaptive control system is needed for blimp robots. The applied research that has studied the features of indoor flying robots
in recent years has prospered. Operating an indoor blimp robot for a long time is difficult because the payload is small,
multiple batteries cannot be stacked, and the design of a thruster that gives freedom to the entire blimp robot is difficult.
Therefore, an autonomous charge that allows operation for a long time is needed. We have developed a method of landing with
orbital control of the charge point that gives autonomy to a blimp robot. The possibility of landing with orbital control
is shown.
This work was presented in part at the 10th International Symposium on Artificial Life and Robotics, Oita, Japan, February
4–6, 2005
An erratum to this article is available at . 相似文献
20.
Xingzhi Wang Fuyuki Sato Keiji Tanimoto Niveda Rajeshwaran Lakshmi Thangavelu Makoto Makishima Ujjal K. Bhawal 《International journal of molecular sciences》2021,22(19)
Periodontal inflammation is a common inflammatory disease associated with chronic inflammation that can ultimately lead to alveolar attachment loss and bone destruction. Understanding autophagy and pyroptosis has suggested their significant roles in inflammation. In recent years, studies of differentiated embryo-chondrocyte expressed genes 1 and 2 (Dec1 and Dec2) have shown that they play important functions in autophagy and in pyroptosis, which contribute to the onset of periodontal inflammation. In this review, we summarize recent studies on the roles of clock genes, including Dec1 and Dec2, that are related to periodontal inflammation and other diseases. 相似文献