Determination of the IgA 7 S/IgA 11 S ratio in human bronchial secretion by thin layer gel filtration immunoelectrophoresis (author's transl)

After extraction by treatment with Ecteola-cellulose, IgA 11 S and IgA 7 S from human bronchial secretion were first separated by thin-layer gel filtration in Ultrogel AcA 3-4, and then were analyzed by crossed immunoelectrophoresis with an immune serum against alpha chain. Precipitation areas were measured separately. A correlation factor was necessary to express IgA 7 S in terms of IgA 11 S: it was calculated with standard curves made with serum and colostral IgA.     

Comparative study of the activity of cobalt and nickel as promoters of HDS catalysts

A series of trimetallic (NiCoMo) hydrodesulphurisation supported catalysts were prepared using a successive impregnation method, varying the ratio of promoters Ni/(Ni + Co) and maintaining the ratio (Ni + Co)/Mo constant. Optima for higher thiophene hydrodesulphurisation activity were found for ratios Ni/(Ni + Co) of 0.7 and 0.8. It is suggested that the optima may be strongly dependent on method of catalyst preparation, and/or type of support employed. Presulphidation was found to increase the activity in NiMo more than in CoMo. Testing of commercial catalysts confirmed various differences between CoMo and NiMo catalysts.     

Oral findings in patients with active or inactive Crohn''s disease

The frequency and type of oral mucosal lesions, dental infections, and salivary constituents were evaluated in 53 patients with Crohn's disease, who were divided into inactive, mildly active, and severely active groups on the basis of clinical and endoscopic criteria. Buccal biopsies from nine patients with active disease showed morphologic changes that suggested Crohn's disease-related lesions. Panoramic radiographs revealed more infectious foci in the teeth of patients with active Crohn's disease than in patients with inactive disease. Salivary flow rate, buffering capacity, total protein, amylase, and IgA and IgG concentrations did not differ with respect to the activity of Crohn's disease. The observed mucosal inflammation in patients with active Crohn's disease, although high in frequency, was mild and did not need therapy, but the great number of dental infections in association with the activity of Crohn's disease should be taken into account in the treatment of these patients.     

The stereochemical configuration of lysobisphosphatidic acid from rat liver, rabbit lung and pig lung

After bilateral lesion of the posterior lateral thalamic nucleus in rats, studies been made of motor-feeding reflexes to light stimuli. Using spatial preference technique, it was shown that lesion of this nucleus (representing a part of the retino-tecto-thalamo-cortical system) disturbs differentiation of spatially uncoupled visual stimuli. These data confirm the idea on telencephalization of ancient retino-tectal part of the visual system in evolution and indicate that this part is involved into the analysis of spatial relationships.     

Biosynthesis and structure of glycosyl diglycerides,steryl glucosides,and acylated steryl glucosides

A particulate enzyme fraction from Mycobacterim smegmatis catalyzed the transfer of -14C-glucose from the UDP--14C-glucose into neutral glycolipids. The two major radioactive components were purified by column chromatography on O-diethylamino ethyl cellulose (acetate) and thin layer chromatography on silica gel in several solvents. The first product yielded a water-soluble component upon saponification, which had a hexoseglycerol ratio of 1:1 with all of the hexose being identified as glucose. The second product yielded a water-soluble component upon saponification which contained hexose and glycerol in a 2:1 ratio and, in addition to glucose, contained lesser amounts of mannose and galactose. Palmitate and oleate were the predominant fatty acids and were present in equimolar amounts. The products thus have been identified as monoglycosyldiglyceride and diglycosyldiglyceride. The diglycosyldiglyceride could also be labeled with -14C-galactose when UDP--14C-galactose served as the donor, but the monoglycosyldiglyceride was only slightly labeled with -14C-galactose. Membrane fractions from mung bean seedlings catalyzed the transfer of -14C-glucose from UDP--14C-glucose into a neutral glycolipid which has been purified by thin layer chromatography and analyzed by combined gas liquid chromatography-mass spectrometry. It was determined to be a steryl glucoside with the two major sterol components being betasitosterol and stigmasterol linked to beta-D-glucose. Particulate fractions from developing cotton fibers also catalyzed the formation of steryl glucosides and, in addition, they catalyzed the esterification of steryl glucosides at the 6 position of glucose with fatty acids (primarily palmitate and oleate) from an andogenous acyl donor. Both the glucosyl transferase and the acyltransferase have been solubilized with Triton X-100 and partially purified by chromatography on Sephadex G-200. The acyltransferase activity was reconstituted by the addition of the steryl glucoside and a phospholipid acyl donor.     

Effects of annealing on performance of InP solar cells on GaAs substrates

Effects of annealing on crystal structure and device characteristics of InP solar cells on GaAs substrates were studied. Two different methods, thermally cycled annealing during the growth, and rapid thermal annealing after the growth, were applied. The results obtained for the annealed solar cells were compared with those obtained for nonannealed and homoepitaxial solar cells. The dislocation density was significantly reduced by annealing from that of the non-annealed heteropitaxial solar cells, and most of the device characteristics were improved.