Towards stable diagnostic setups in clinical proteomics: Absolute quantitation of peptide biomarkers using MALDI‐TOF‐MS

In routine clinical diagnostics, peptide biomarkers are most commonly quantified using immunological techniques but these methods often lack sensitivity and/or specificity. Hence, quantitative mass spectrometry detection is desirable as an alternative diagnostic tool. To date, quantitative mass spectrometry is mostly based on ESI‐MS coupled to LC, requiring highly sophisticated instrumentation and knowledge and is time consuming and expensive. In contrast, MALDI‐TOF‐MS is a very simple, sensitive and rapid method for the detection of peptide biomarkers. However, the infeasibility of absolute quantification has been a tremendous handicap to the use of MS in stable clinical diagnostics. Here, we describe the development of a technical platform based on ClinProt particles and heavy‐isotope internal peptide standards for the fast and reliable preparation of samples. This combines the advantages of MALDI‐TOF as a read‐out system with absolute quantitation of peptide biomarkers. As a proof‐of‐concept, this platform was successfully employed for the absolute determination of the concentration of the highly abundant serum peptide des‐Ala‐Fibrinopeptide A in 45 serum samples from healthy donors. Such technology essentially contributes to the development of a stable MALDI‐TOF‐MS‐based clinical assay.     

Remote Laboratory Architecture for the Validation of Industrial Control Applications

The seven engineering degrees of the Higher Technical School of Design Engineering (ETSID), Technical University of Valencia, include in their formation programs, subjects, and laboratory projects to instruct students in the aspects of the design, development, and validation of applications for process control, automation, industrial informatics, and embedded systems. Moreover, the authors participate in European projects of education such as the International Network of Embedded System (INES) and the European Project Semester (EPS), where exchange students remotely perform the first phase of their projects at ETSID from their home universities. To significantly reduce the cost of installing a huge number of real prototypes in labs and to fulfill the distance requirements of the exchange students, a remote laboratory architecture, i.e., simPROCes, has been designed. simPROCes not only permits the teleoperation of simulators/real prototypes but also allows that complete control applications be remotely tested and validated. simPROCes is precisely specified to be independent of the model of computer, data acquisition card, programming language, and operating system, and is transparent to the programmer and easy to use. This system is useful both for the education and development of control application purposes. A water tank process shows the installation procedure of simPROCes to test and validate control applications. The experience of applying simPROCes in the electronic engineering degree and within the framework of INES and EPS has been successfully rated by student surveys.     

Performance of a proposed complete wetting surface counter flow channel type liquid desiccant air dehumidifier

An idea that improves the wettability over the surfaces of a cylindrical dehumidifier channel was proposed and experimentally proved. Fibrous sheets were attached to the inner surfaces of the channel. The capillary effect of fibers sustains the complete wetting of the heat and mass transfer surfaces. The air to be dehumidified and cooled flows upward in the annulus space between the two layers of fibrous sheets, which are saturated with the downward flowing desiccant solution. The permeability of the fibrous sheet was determined experimentally. It was 2.43 × 10^?10 m². The measured solution flow rate due to the capillary suction of the sheets was Γ_in,min = 1.12 kg/h m. The liquid desiccant tested was H₂O/CaCl₂ with salt concentration ratios ranging from 35 to 40%. The measured distribution of the solution flow rate along the circumference of the sheets at the outlet showed 5% deviation from the average flow rate. This is a good indication for the good wettability of walls inside the dehumidifier.Feeding the solution by this mechanism has many advantages over spray feeding. Beside sustaining complete surface wetting, it also prevents channel blockage with solution, which is a main factor in increasing the air pressure drop. About 95% of the air pressure drop is saved in this study by avoiding these problems. A simple theoretical model for the heat and mass transfer processes inside the dehumidifier was developed and experimentally validated. In general, there is good agreement between the predicted and measured data. The developed model was utilized to study the effect of the different parameters on the dehumidifier performance. For a 1 m height dehumidifier with an inlet specific humidity and air temperature of 0.0234 kg_v/kg_a, and 35 °C, respectively, the predicted outlet air specific humidity was 0.0102 kg_v/kg_k and the corresponding outlet air temperature was 27.4 °C. The inlet solution temperature and salt concentration were 25 °C and 40%, respectively.     

A Web-based integrated design system: its applications on conceptual design stage

This paper describes the development of a Web-based integrated system for collaborative product development evolving from marketing analysis to prototype generation. The proposed system encompasses a marketing information system (MIS), a human resources management (HRM) system, a supply-chain management (SCM) system, a communication media, an integrated product design studio, a user interface and databases. It enables project planners, marketing analysts, designers, suppliers, and manufacturing planners to work at the early stages to reduce any unnecessary wasted time, resources, and costs, thus increasing the total product quality, maximising the organisation resources used, and reducing the total product cost and product lead time to better face global competition. The tangible advantage of implementing this system is that it provides an integrated environment for total product development from concept to realisation. Therefore, an efficient product development process is generated. One case study is demonstrated and discussed to validate the proposed system.     

Experimental Investigation of the Grain Noise in Interferometric Detection of Ultrasonic Waves

Besides their other obvious advantages over conventional ultrasonic sensors, laser interferometers offer optical diffraction limited apertures that are far smaller than the acoustic wavelength in the specimen under inspection. This unique feature can be exploited for the purposes of super-resolution near-field acoustic microscopy, which detects the rapidly decaying evanescent vibrations produced by surface and near-surface scatterers such as small fatigue cracks, pores, anomalous grains, etc. However, higher resolution also means higher sensitivity to inherent microscopic material inhomoge-neities. In this paper, experimental results are presented for the incoherent material noise in 2024 aluminum and Ti-6Al-4V titanium alloys at two different nominal frequencies of 5 and 10 MHz. It is shown that the incoherent grain noise significantly increases as the illuminated spot size decreases. Above the acoustic wavelength, the observed phenomenon is mainly due to the increasing sensitivity of the receiver to propagating scattered waves generated in the interior of the specimen. Below the acoustic wavelength, the further increasing material noise is mainly due to evanescent vibrations caused by nearby scatterers.     

Strength and deformation characteristics and small strain properties of undisturbed gravelly soils

A series of medium-scale and large-scale triaxial and unconfined compression tests was conducted in order to evaluate the strength and deformation characteristics and small strain properties of undisturbed well-graded gravelly soils retrieved from three tunnel excavation sites in Toyama prefecture, Japan. Undisturbed gravelly soils were taken by means of a new sampling method using thick water-soluble polymer solutions. The strength and deformation characteristics were evaluated mainly by performing sustained loading and large amplitude unloading and reloading cycles during otherwise monotonic loading at a constant strain rate in drained triaxial compression tests. During isotropic consolidation and shearing, at several stress states, eleven very small vertical cycles were applied to evaluate the quasi-elastic deformation property at small strain levels around 0.001% by static measurement. Dynamic measurements using a pair of accelerometers attached to the side surface of the specimen and wave sources attached to the top cap were also conducted at the same stress levels as static measurements in a single test. Several effects including grading characteristics and pressure level on the difference between the moduli measured statically and dynamically were discussed. The relationship between the small strain and strength properties of undisturbed gravelly soils was evaluated. The small strain properties of air-dried dense Toyoura sand in large-scale triaxial compression tests were also investigated in this study to compare the results of undisturbed gravelly soils.     

Co-occurrence of mycotoxins in commercial formula milk and cereal-based baby food on the Qatar market

The present study was conducted to explore the occurrence of mycotoxins in commercial baby foods in Doha-Qatar. LCMS/MS- and HPLC-based analysis of baby food (n = 67) for 12 mycotoxins confirmed the presence of aflatoxin M1 (AFM1, 33%), ochratoxin A (OTA, 31%), deoxynivalenol (DON, 27%), aflatoxin B1 (AFB1, 22%), fumonisin B2 (FB2, 10%), zearalenone (ZEN, 4%) and T-2 toxin (2%). Noodles exhibited the maximum contamination percentage, with 33% of the samples being contaminated above the EU maximum limits, for at least one mycotoxin. Among the multi-grain flake samples, up to 28% and for the milk and milk-based-cereal samples, 14% contained at least one mycotoxin above the EU maximum limits. From all cereal-based food samples, 22%, 5%, 2% and 2% were concurrently contaminated with 2, 3, 4 and 5 mycotoxins, respectively. The occurrence of toxicological important mycotoxins in Qatari market warrants the implementation of strict regulatory limits to protect human health.     

A dynamic joint scheduling and call admission control scheme for IEEE 802.16 networks

We propose a dynamic joint scheduling and call admission control (CAC) scheme for service classes defined in IEEE 802.16 standard. Using priority functions, equipped with service weights and service arrival rates, the proposed scheduling scheme differentiates service classes from each other. Based on obtained priority values, we first allocate the achievable bandwidth proportionally. Within individual service classes, we then use appropriate local schedulers to transmit packets accordingly. Moreover, instead of immediate admitting or blocking a new connection request, the proposed CAC scheme computes the average transmission rate that can be allocated to that connection during a time interval. The connection is admitted if its required rate is satisfied while at the same time QoS requirements of ongoing connections are not violated. Our numerical results demonstrate the effectiveness of the proposed schemes compared to the other schemes in the literature.     

Correlation between nanoindentation response and wear characteristics of CrN-based coatings deposited by an Arc-PVD method

The examination of the existing relationships between nanoindentation responses and tribological properties of the nanostructured CrN, Cr(CN), and (CrTi)N coatings was the matters to be considered in this research. A cathodic arc physical vapor deposition machine was therefore implemented to apply the chosen coatings on the DIN 1.2510 tool steel substrate. Moreover, an X-ray diffraction and a field emission scanning electron microscope were utilized in order to show the features regarding microstructure and morphology of these very coatings. The mechanical and tribological behavior of the coatings was expected to be assessed with the use of a nanoindentation and pin-on-disc wear tests. According to the obtained result, the wear resistance and hardness value of the (CrTi)N coating were proved to be much better than those of the CrN and Cr(CN). Linear equations were proposed between wear rate/hardness and friction coefficient/hardness to evaluate the correlation between mechanical and tribological properties. The presence of a quadratic equation between the friction coefficient and the plastic deformation index was also discovered.