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991.
目前对人工机械心脏瓣膜血液动力学特性的测试方法,大部分是采用实验装置进行的.针对模拟结果由于实验装置的不同而可能存在的差异,本研究通过使用SolidWorks软件中的插件COSMOSFloWorks,按照国际标准ISO5840-2005规定的试验条件,通过模拟实验对曾出现的笼球瓣、笼碟瓣、斜碟瓣和双叶瓣进行血液动力学特性进行测试.在压力均为200 mmHg的情况下,笼球瓣和笼碟瓣的跨瓣压差均大于40 mmHg,相应的有效开口面积约为1 cm2;斜碟瓣和双叶瓣跨瓣压差均小于20 mmHg,斜碟瓣的有效开口面积为1.5~2.1 cm2,双叶瓣为2.4~3.2 cm2,但它的返流百分比相对来说比较大.分析结果与通过试验装置进行测试的结果相一致,证实这种研究方法的可行性,可以为研制和开发新型人工机械心脏瓣膜提供一种实用工具. 相似文献
992.
993.
少量Sc对Al-Mg-Cu-Li-Zr合金组织与性能的影响 总被引:2,自引:0,他引:2
通过显微硬度测试、拉伸试验、SEM、TEM及能谱等方法研究了添加少量Sc对Al-4.0Mg-1.5Cu-1.0Li-0.12Zr合金微观组织与性能的影响.结果表明,Sc的加入能显著提高合金硬度、强度和塑性;促进时效初期δ'(Al3Li)相的均匀析出,形成尺寸细小的δ'相和尺寸较大的Al3Li/Al3(Sc,Zr)复合相,并延缓了δ'相的长大;Sc的添加还促进过时效阶段S'相的弥散析出,延缓软化进程.Z相的形成与时效温度密切相关,而与Ag的添加并无直接关系. 相似文献
994.
995.
Lanlan Li Mingfei Yang Jia Yu Sha Cheng Mashaal Ahmad Caihong Wu Xinwei Wan Bixue Xu Yaacov Ben-David Heng Luo 《International journal of molecular sciences》2022,23(18)
Prostate cancer (PCa) is a common malignant cancer of the urinary system. Drug therapy, chemotherapy, and radical prostatectomy are the primary treatment methods, but drug resistance and postoperative recurrence often occur. Therefore, seeking novel anti-tumor compounds with high efficiency and low toxicity from natural products can produce a new tumor treatment method. Matijin-Su [N-(N-benzoyl-L-phenylalanyl)-O-acetyl-L-phenylalanol, MTS] is a phenylalanine dipeptide monomer compound that is isolated from the Chinese ethnic medicine Matijin (Dichondra repens Forst.). Its derivatives exhibit various pharmacological activities, especially anti-tumor. Among them, the novel MTS derivative HXL131 has a significant inhibitory effect against prostate tumor growth and metastasis. This study is designed to investigate the effects of HXL131 on the growth and metastasis of human PCa cell lines PC3 and its molecular mechanism through in vitro experiments combined with proteomics, molecular docking, and gene silencing. The in vitro results showed that HXL131 concentration dependently inhibited PC3 cell proliferation, induced apoptosis, arrested cell cycle at the G2/M phase, and inhibited cell migration capacity. A proteomic analysis and a Western blot showed that HXL131 up-regulated the expression of proliferation, apoptosis, cell cycle, and migration-related proteins CYR61, TIMP1, SOD2, IL6, SERPINE2, DUSP1, TNFSF9, OSMR, TNFRSF10D, and TNFRSF12A. Molecular docking, a cellular thermal shift assay (CETSA), and gene silencing showed that HXL131 had a strong binding affinity with DUSP1 and TNFSF9, which are important target genes for inhibiting the growth and metastasis of PC3 cells. This study demonstrates that HXL131 exhibited excellent anti-prostate cancer activity and inhibited the growth and metastasis of prostate cancer cells by regulating the expression of DUSP1 and TNFSF9. 相似文献
996.
997.
998.
Zhi He Li Zheng Qiqi Chen Sen Xiong Zhide He Jiaxiang Hu Zhijun Ma Qian Zhang Jiayang He Lijuan Ye Liang He Jie Luo Xiaobin Gu Mingwang Zhang Ziting Tang Yuanyuan Jiao Yong Pu Jinxin Xiong Kuo Gao Bolin Lai Shiyong Yang Deying Yang Taiming Yan 《International journal of molecular sciences》2022,23(23)
Bmpr2 plays a central role in the regulation of reproductive development in mammals, but its role during ovarian development in fish is still unclear. To ascertain the function of bmpr2 in ovarian development in the ricefield eel, we isolated and characterized the bmpr2 cDNA sequence; the localization of Bmpr2 protein was determined by immunohistochemical staining; and the expression patterns of bmpr2 in ovarian tissue incubated with FSH and hCG in vitro were analyzed. The full-length bmpr2 cDNA was 3311 bp, with 1061 amino acids encoded. Compared to other tissues, bmpr2 was abundantly expressed in the ovary and highly expressed in the early yolk accumulation (EV) stages of the ovary. In addition, a positive signal for Bmpr2 was detected in the cytoplasm of oocytes in primary growth (PG) and EV stages. In vitro, the expression level of gdf9, the ligand of bmpr2, in the 10 ng/mL FSH treatment group was significantly higher after incubation for 4 h than after incubation for different durations. However, bmpr2 expression in the 10 ng/mL FSH treatment group at 2 h, 4 h and 10 h was significantly lower. Importantly, the expression level of bmpr2 and gdf9 in the 100 IU/mL hCG group had similar changes that were significantly decreased at 4 h and 10 h. In summary, Bmpr2 might play a pivotal role in ovarian growth in the ricefield eel, and these results provide a better understanding of the function of bmpr2 in ovarian development and the basic data for further exploration of the regulatory mechanism of gdf9 in oocyte development. 相似文献
999.
Zengpeng Han Nengsong Luo Yang Wu Jiaxin Kou Wenyu Ma Xin Yang Yuxiang Cai Lin Ma Lu Han Xiujie Wang Hualing Qin Qing Shi Jie Wang Chaohui Ye Kunzhang Lin Fuqiang Xu 《International journal of molecular sciences》2022,23(21)
As powerful tools for local gene delivery, adeno-associated viruses (AAVs) are widely used for neural circuit studies and therapeutical purposes. However, most of them have the characteristics of large diffusion range and retrograde labeling, which may result in off-target transduction during in vivo application. Here, in order to achieve precise gene delivery, we screened AAV serotypes that have not been commonly used as gene vectors and found that AAV13 can precisely transduce local neurons in the brain, with a smaller diffusion range than AAV2 and rigorous anterograde labeling. Then, AAV13-based single-viral and dual-viral strategies for sparse labeling of local neurons in the brains of C57BL/6 or Cre transgenic mice were developed. Additionally, through the neurobehavioral test in the ventral tegmental area, we demonstrated that AAV13 was validated for functional monitoring by means of carrying Cre recombinase to drive the expression of Cre-dependent calcium-sensitive indicator. In summary, our study provides AAV13-based toolkits for precise local gene delivery, which can be used for in situ small nuclei targeting, sparse labeling and functional monitoring. 相似文献
1000.
Yingjie Luo Yiwei Zhang Cimin Tao Hongfei Ni Xuesong Liu Yong Chen Yongjiang Wu Hang Song Tengfei Xu 《International journal of molecular sciences》2022,23(20)
Imidazolium-based ionic liquids are wildly used in natural product adsorption and purification. In this work, one typical polymeric ionic liquid (PIL) was synthesized by using L-proline as the anion, which exhibited excellent adsorption capacity toward tea polyphenol epigallocatechin gallate (EGCG). The adsorption conditions were optimized with the response surface method (RSM). Under the optimum conditions, the adsorption capacity of the PIL for EGCG can reach as high as 552 mg/g. Dynamics and isothermal research shows that the adsorption process of EGCG by the PIL particularly meets the quasi-second-order kinetic equation and monolayer adsorption mechanism. According to thermodynamic parameter analysis, the adsorption process is endothermic and spontaneous. The results of theoretical calculation by molecular docking also demonstrated the interaction mechanisms between EGCG and the ionic liquid. Considering the wide application of imidazolium-based ionic liquids in component adsorption and purification, the present study can not only be extended to other similar experimental mechanism validation, but also be representative for guiding the synthesis of PIL and optimization of adsorption conditions. 相似文献