Polymer optical fiber for near infrared use

Using the empirical simulation that we developed to estimate loss spectra of amorphous polymers, we predicted the intrinsic loss spectrum of fluorine-containing polyarylate (FPAr) that should have a small attenuation loss in the near infrared region. Loss of FPAr is smaller than that of poly (methyl methacrylate), which is a typical optical polymer used as a core material in polymer optical fiber (POF), above 720 nm. FPAr was synthesized by phase transfer catalyzed polycondensation of 2,2-bis (4-hydroxyphenyl)-1,1,1,3,3,3-hexafluoropropane with isophthaloyl dichloride. Its glass-transition temperature (T_g) by DSC is 163°C. This indicates that FPAr has higher heat resistance than polycarbonate (PC) by about 10°C. A film of 100μm thick has over 85% transmittance from 330 to 900 nm. Values from a tension test are similar to those of PC. As a result, FPAr is expected to be a good core polymer for POF, because of its high T_g, good transparency, and good flexibility. Next, the POF made of FPAr was fabricated by a melt-flow spinning method. It had larger attenuation loss than predicted. The difference from the simulation was extrinsic loss caused by the fabricating process. Therefore, when a more suitable fabricating process is developed, loss of FPAr-POF can be decreased, so that it will be applicable to optical communications in the near infrared region. © 1993 John Wiley & Sons, Inc.     

A-STAR: Toward translating Asian spoken languages

This paper outlines the first Asian network-based speech-to-speech translation system developed by the Asian Speech Translation Advanced Research (A-STAR) consortium. Eight research groups comprising the A-STAR members participated in the experiments, covering nine languages, i.e., eight Asian languages (Hindi, Indonesian, Japanese, Korean, Malay, Thai, Vietnamese, and Chinese) and English. Each A-STAR member contributed one or more of the following spoken language technologies: automatic speech recognition, machine translation, and text-to-speech through Web servers. The system was designed to translate common spoken utterances of travel conversations from a given source language into multiple target languages in order to facilitate multiparty travel conversations between people speaking different Asian languages. It covers travel expressions including proper nouns that are names of famous places or attractions in Asian countries. In this paper, we describe the issues of developing spoken language technologies for Asian languages, and discuss the difficulties involved in connecting different heterogeneous spoken language translation systems through Web servers. This paper also presents speech-translation results including subjective evaluation, from the first A-STAR field testing which was carried out in July 2009.     

Reliability-guided Rayleigh backscattering spectrum correlation method for distributed strain measurements in optical fibres

We present a method of reliability-guided Rayleigh backscattering correlation for distributed strain measurements in optical fibres. In this method, a reference Rayleigh-backscattering-spectrum (RBS) range that is larger than the measurement RBS range is defined to extend the matching range. To obtain the best match between reference and measurement spectra, the zero-mean normalized cross correlation (ZNCC) is employed to evaluate the degree of similarity. The path for searching the maximum similarity matching pattern is guided using Newton’s iteration method. The reliability of the computed RBS shift is identified by the ZNCC coefficient distribution. The experiments show that the proposed method has high reliability in computing the RBS shift. Even at a relatively large strain (e.g. 5000?µ?), the proposed method can stably demodulate the strain within a relative error of ?1% and a spatial resolution of 1.6?cm over a 22-meter-long single-mode fibre. This shows that the proposed method has an advantage in regard to relatively large strain measurements.     

Topology optimization for designing strain-gauge load cells

Load cells are used extensively in engineering fields. This paper describes a novel structural optimization method for single- and multi-axis load cell structures. First, we briefly explain the topology optimization method that uses the solid isotropic material with penalization (SIMP) method. Next, we clarify the mechanical requirements and design specifications of the single- and multi-axis load cell structures, which are formulated as an objective function. In the case of multi-axis load cell structures, a methodology based on singular value decomposition is used. The sensitivities of the objective function with respect to the design variables are then formulated. On the basis of these formulations, an optimization algorithm is constructed using finite element methods and the method of moving asymptotes (MMA). Finally, we examine the characteristics of the optimization formulations and the resultant optimal configurations. We confirm the usefulness of our proposed methodology for the optimization of single- and multi-axis load cell structures.     

Geometrical design of thermoelectric generators based on topology optimization

This paper discusses an application of the topology optimization method for the design of thermoelectric generators. The proposed methodology provides the optimized geometry in accordance with various arbitrary conditions such as the types of materials, the volume of materials, and the temperature and shape of the installation position. By considering the coupled equations of state for the thermoelectric problem, we introduce an analytical model subject to these equations, which mimics the closed circuit composed of thermoelectric materials, electrodes, and a resistor. The total electric power applied to the resistor and the conversion efficiency are formulated as objective functions to be optimized. The proposed optimization method for thermoelectric generators is implemented as a geometrical optimization method using the solid isotropic material with penalization method used in topology optimizations. Simple relationships are formulated between the density function of the solid isotropic material with penalization method and the physical properties of the thermoelectric material. A sensitivity analysis for the objective functions is formulated with respect to the density function and the adjoint equations required for calculating it. Depending on the sensitivity, the density function is updated using the method of moving asymptotes. Finally, numerical examples are provided to demonstrate the validity of the proposed method. Copyright © 2012 John Wiley & Sons, Ltd.     

Expression and purification of recombinant human annexin A2 in Pichia pastoris and utility of expression product for detecting annexin A2 antibody

Annexin A2, a Ca2+-dependent phospholipid binding protein, is abundantly expressed in various human organs, which exists as either a membrane-associated, cytosolic or soluble form in serum. We constructed expression systems for recombinant human annexin A2 (rhA2) using Pichia pastoris. The systems are designed to secrete rhA2 as either the N- or C-terminally His6-tagged form to facilitate purification. Both types of rhA2 were overexpressed, but in the N-terminal-truncated form as revealed from the results of N-terminal amino acid sequencing and Western blotting. Therefore, further purification of N-terminally His6-tagged rhA2 was not feasible because of the removal of the N-terminal His6-tag sequence. C-terminally His6-tagged rhA2 was expressed as either a glycosylated or a nonglycosylated form, and the nonglycosylated form was purified using the combination of nickel-immobilized affinity, concanavalin A and cation exchanged column chromatographies. The solid-phase binding of rhA2 was examined by enzyme-linked immunosorbent assay (ELISA), which revealed the specific reactivity of rhA2 against an anti-annexin A2 monoclonal antibody. These results suggest that the expression system using P. pastoris is useful for the preparation of rhA2 that is applicable to the ELISA detection of the anti-annexin A2 antibody.     

Temperature programmed desorption study of adsorbed species formed by the decomposition of N2O on ion-exchanged copper zeolite catalysts

The nature of the adsorbed species on Cu-ZSM-5 (Cu-Z), Cu-Mordenite (Cu-M), and Cu-Y-zeolite (Cu-Y) was investigated by means of temperature programmed desorption (TPD). When dinitrogen monoxide (N₂O) came into contact with Cu-zeolites above 573 K, the decomposition of N₂O occurred accompanied by the formation of adsorbed oxygen species and adsorbed nitrogen oxide species. In the TPD runs, three O₂ desorption peaks appeared at temperatures of 623, 673, and 753 K and were named -, β-, and γ-peaks, respectively. The O₂ desorption at the - and γ-peaks became quickly saturated after contacting N₂O at 598 K, while the amount of O₂ desorbed at the β-peak increased with time, not reaching a constant level until 120 min of exposure. The activity for the decomposition of N₂O decreased with the accumulation of β-oxygen over the catalyst. The rate of N₂O decomposition depended upon the nature and amount of the copper zeolite catalysts available, as determined by the formation of - and/or β-oxygen.     

Comparative study on corpora for speech translation

This paper investigates issues in preparing corpora for developing speech-to-speech translation (S2ST). It is impractical to create a broad-coverage parallel corpus only from dialog speech. An alternative approach is to have bilingual experts write conversational-style texts in the target domain, with translations. There is, however, a risk of losing fidelity to the actual utterances. This paper focuses on balancing a tradeoff between these two kinds of corpora through the analysis of two newly developed corpora in the travel domain: a bilingual parallel corpus with 420 K utterances and a collection of in-domain dialogs using actual S2ST systems. We found that the first corpus is effective for covering utterances in the second corpus if complimented with a small number of utterances taken from monolingual dialogs. We also found that characteristics of in-domain utterances become closer to those of the first corpus when more restrictive conditions and instructions to speakers are given. These results suggest the possibility of a bootstrap-style of development of corpora and S2ST systems, where an initial S2ST system is developed with parallel texts, and is then gradually improved with in-domain utterances collected by the system as restrictions are relaxed.     

Single-cell analysis by a scanning thermal lens microscope with a microchip: direct monitoring of cytochrome c distribution during apoptosis process

We developed a microsystem for cell experiments consisting of a scanning thermal lens microscope detection system and a cell culture microchip. The microchip system was good for liquid control in microspace, and this results in secure cell stimulation and coincident in vivo observation of the cell responses. The system could detect nonfluorescent biological substances with extremely high sensitivity without any labeling materials and had a high spatial resolution of approximately 1 microm. This system was applied to monitoring of cytochrome c distribution in a neuroblastoma-glioma hybrid cell cultured in the microflask (1 mm x 10 mm x 0.1 mm; 1 microL) fabricated in a glass microchip. Cytochrome c release from mitochondria to cytosol during the apoptosis process was successfully monitored with this system. The cytochrome c detected with this system was estimated to be approximately 10 zmol. We concluded that the system was suitable for measuring the distribution of chemical substances in a single cell because the microchip is good for liquid handling in microspace and the thermal lens microscope has high sensitivity and spatial resolution.