Aedes (Ochlerotatus) surcoufi (Theobald, 1912). Restoration of the binomen; morphological analysis; position in the holarctic "excrucians" complex (author's transl)

The study of the morphology of eggs, larvae, pupae and adults of Aedes excrucians from Germany, Italy and France has led the authors to rise again the binomen Aedes surcoufi (Theobald, 1912) to particularize the european populations. At the same time, the characteristics of the different species of the Aedes excrucians complex are discussed.     

Accepting its random coil nature allows a partial NMR assignment of the neuronal Tau protein

A combined strategy to obtain a partial NMR assignment of the neuronal Tau protein is presented. Confronted with the extreme spectral degeneracy that the spectrum of this 441 amino acid long unstructured protein presents, we have introduced a graphical procedure based on residue type-specific product planes. Combining this strategy with the search for pairwise motifs, and combining the spectra of different Tau isoforms and even of peptides derived from the native sequence, we arrive at a partial assignment that is sufficient to map the interactions of Tau with its molecular partners. The obtained assignments equally confirm the absence of regular secondary structure in the isolated protein.     

Remote fluorescence imaging of dynamic concentration profiles with micrometer resolution using a coherent optical fiber bundle

Dynamic concentration profiles within the diffusion layer of an electrode were imaged in situ using fluorescence detection through a multichannel imaging fiber. In this work, a coherent optical fiber bundle is positioned orthogonal to the surface of an electrode and is used to report spatial and temporal micrometric changes in the fluorescence intensity of an initial fluorescent species. The fluorescence signal is directly related to the local concentration of a redox fluorescent reagent, which is electrochemically modulated by the electrode. Fluorescence images are collected through the optical fiber bundle during the oxidation of tris(2,2'-bipyridine)ruthenium(II) to ruthenium(III) at a diffusion-limited rate and allow the concentration profiles of Ru(II) reagent to be monitored in situ as a function of time. Tris(2,2'-bipyridine)ruthenium(II) is excited at 485 nm and emits fluorescence at 605 nm, whereas the Ru(III) oxidation state is not fluorescent. Our experiments emphasize the influence of two parameters on the micrometer spatial resolution: the numerical aperture of optical fibers within the bundle and the Ru(II) bulk concentration. The extent of the volume probed by each individual fiber of the bundle is discussed qualitatively in terms of a primary inner-filter effect and refractive index gradient. Experimentally measured fluorescence intensity profiles were found to be in very good agreement with concentration profiles predicted upon considering planar diffusion and thus validate the concept of this new application of imaging fibers. The originality of this remote approach is to provide a global view of the entire diffusion layer at a given time through one single image and to allow the time expansion of the diffusion layer to be followed quantitatively in real time.     

Aerosol lidar intercomparison in the framework of the EARLINET project. 1. Instruments

In the framework of the European Aerosol Research Lidar Network to Establish an Aerosol Climatology (EARLINET), 19 aerosol lidar systems from 11 European countries were compared. Aerosol extinction or backscatter coefficient profiles were measured by at least two systems for each comparison. Aerosol extinction coefficients were derived from Raman lidar measurements in the UV (351 or 355 nm), and aerosol backscatter profiles were calculated from pure elastic backscatter measurements at 351 or 355, 532, or 1064 nm. The results were compared for height ranges with high and low aerosol content. Some systems were additionally compared with sunphotometers and starphotometers. Predefined maximum deviations were used for quality control of the results. Lidar systems with results outside those limits could not meet the quality assurance criterion. The algorithms for deriving aerosol backscatter profiles from elastic lidar measurements were tested separately, and the results are described in Part 2 of this series of papers [Appl. Opt. 43, 977-989 (2004)]. In the end, all systems were quality assured, although some had to be modified to improve their performance. Typical deviations between aerosol backscatter profiles were 10% in the planetary boundary layer and 0.1 x 10(-6) m(-1) sr(-1) in the free troposphere.     

From data topology to a modular classifier

This article describes an approach to designing a distributed and modular neural classifier. This approach introduces a new hierarchical clustering that enables one to determine reliable regions in the representation space by exploiting supervised information. A multilayer perceptron is then associated with each of these detected clusters and charged with recognizing elements of the associated cluster while rejecting all others. The obtained global classifier is comprised of a set of cooperating neural networks and completed by a K-nearest neighbor classifier charged with treating elements rejected by all the neural networks. Experimental results for the handwritten digit recognition problem and comparison with neural and statistical nonmodular classifiers are given.Received: 1 October 2002, Accepted: 21 November 2002, Published online: 6 June 2003     

A novel packaging approach for RF MEMS switching functions on alumina substrate

This paper reports the design of a hermetic-compatible wafer-scale package for RF MEMS based components. The presented packaging concept consists in encapsulating the whole RF device or subsystem instead of encapsulating each MEMS component separately, which will reduce the device size and cost. This approach is based on the MEMS fabrication technology on ceramic substrate and the use of laser drilled vias hole techniques to realize full metallized vias in alumina substrates. These vias holes will allow a low loss RF signal transmission inside the package without breaking its hermeticity. Hence, several packaged switching networks prototypes, based on ohmic contact MEMS switches, have been designed following this approach and the packaging electromagnetic impact on these components has been especially studied to result on good performance devices.     

A hybrid method for detecting the onset of local necking by monitoring the bulge forming load

Strain-based Forming Limit Diagrams (FLD), which are typically obtained under linear or quasi-linear loading conditions, describe the limiting strains in terms of the major and minor in-plane strains before the onset of necking or the final failure (FFD). These strains can be detected by analysing the strain field in the vicinity of necking or cracking defects. It has generally been agreed that the loading versus time signal is not suitable for detecting necking processes. A novel hybrid method of detecting the onset of necking based on the experimental and simulated bulging load is presented in this paper. This method consists mainly in comparing the experimental forming load, i.e., a load showing plastic instability, with the numerical predictions obtained by performing finite element simulation. The simulation of the bulging process does not include any damage or failure criteria. A homogeneous forming load can therefore be simulated without requiring any information about the localization. This method was applied to detecting the onset of local necking in circular and elliptic quasistatic bulge tests on sheet material, with a diameter of 200 mm. Two materials were tested, a 0.8 mm thick DP450 Dual Phase steel sheet and a 1 mm thick AA6016-T4 aluminium sheet. The onset of necking observed with our method was compared with the results obtained by performing Hogström’s analysis based on the measured strain field over time and similar necking strains were obtained. Beside, the Bressian Williams Hill (BWH) shear criterion was identified for each test from experimental results. A slight scattering of the shear stress values was observed.     

New potential for preparation of performing h-BN coatings via polymer pyrolysis in RTA furnace

An innovative IR irradiation annealing process is used to increase the crystallization ratio of hexagonal boron nitride (h-BN) coatings on pure titanium pellets. Since the polymer pyrolysis route requires heating the green polymer at high temperature to convert it into a ceramic, the use of IR radiation furnace (compared to a resistive furnace) allows achievement of better crystallized h-BN while the substrate remains at relatively low temperature (<1000 °C). Annealing treatments have been performed under argon or nitrogen using either a halogen lamp or a Rapid Thermal Annealing (RTA) furnace. Advanced structural and chemical characterizations have shown a good chemical stability of the coatings. In addition, it has been revealed that samples annealed under Ar present a micro-composite structure at the interphase composed of a μ-layer of TiB₂/TiB/Ti(N)_x between the coating and the substrate, whereas samples annealed under nitrogen display a simpler structure at the interphase, with only TiN.     

Desorption of large molecules with light-element clusters: Effects of cluster size and substrate nature

This contribution focuses on the conditions required to desorb a large hydrocarbon molecule using light-element clusters. The test molecule is a 7.5 kDa coil of polystyrene (PS61). Several projectiles are compared, from C₆₀ to 110 kDa organic droplets and two substrates are used, amorphous polyethylene and mono-crystalline gold. Different aiming points and incidence angles are examined. Under specific conditions, 10 keV nanodrops can desorb PS61 intact from a gold substrate and from a soft polyethylene substrate. The prevalent mechanism for the desorption of intact and ‘cold’ molecules is one in which the molecules are washed away by the projectile constituents and entrained in their flux, with an emission angle close to ∼70°. The effects of the different parameters on the dynamics and the underlying physics are discussed in detail and the predictions of the model are compared with other published studies.