A high performance liquid chromatography method for simultaneous detection of 20 bioactive components in tea extracts

Tea is the second most widely consumed beverage and contains various bioactive compounds. A simple method to analyze these compounds is of great scientific and commercial interest. In this work, a 30 min HPLC method was developed using a simple gradient elution system, and the mobile phases and elution gradients were optimized. This method separated 17 polyphenols and three alkaloid compounds in tea extracts, including catechins, alkaloids, phenolic acids, flavonols, and flavone, which are responsible for the bioactivity and flavor of tea. Excellent linearity was observed for all standard calibration curves, and correlation coefficients were above 0.9994. Heatmap analysis demonstrated significant separation between green, black, and pu‐erh tea samples. The method described here is accurate and sensitive enough for the determination of active components in tea and could potentially be applied to other food products for the comprehensive investigation of their quality.     

Positive solutions of impulsive boundary value problems for first-order nonlinear functional differential equations

In this paper, we study the problem on the existence of positive solutions for a class of impulsive periodic boundary value problems of first-order nonlinear functional differential equations. By using the fixed point theorem in cones and some analysis techniques, we present some sufficient conditions which guarantee the existence of one and multiple positive solutions for the impulsive periodic boundary value problems. Our results generalize and improve some previous results. Moreover, our results show that positive solutions for the impulsive periodic boundary value problems may be yielded completely by some proper impulsive conditions (see Example 4.1 and Remark 4.2 in Sect. 4), and also implies that proper impulsive conditions are of great significance to simulate processes, optimal control, population model and so on.     

Correlation between atomic size and elastic properties/glass transition temperature in metallic glasses

<正>As a relative newcomer in the glass family,metallic glass challenges our standpoint on crystalline metals and extends our knowledge concerning the nature of glasses[1].Metallic glasses have attracted considerable attention because of their unique mechanical,physical,and chemical properties[2-4].Newly developed bulk metallic glass can combine toughness and strength beyond the benchmark ranges established by the     

3,5-二甲基-亚硝基哌嗪代谢物对DNA烷化作用的理论研究

用从头计算法在MP2/6-311＋G(d,p)水平上对3,5-二甲基-亚硝基哌嗪(DMNP)及其类似物经代谢生成DNA烷化剂的机理进行了研究. 探讨了N’原子上取代基的变化对DMNP代谢物生成α-位和γ-位两个烷化中心的影响, 解释了两个烷化中心的生成活性与化合物的致癌性之间的关系. 结果表明, α-位和γ-位代谢物越容易生成活泼亲电中间体, 其母体化合物的致癌活性越高. 但如果有一个烷化中心丧失活性, 就会明显减弱化合物的致癌活性. 因此, DMNP类化合物的致癌性取决于其α-位和γ-位的协同烷化作用, 在评价DMNP的致癌强度时需同时考虑两个烷化中心的烷化能力以及二者之间的关系.     

DSC法测定PS玻璃化温度的再研究

对Fevotte提出的由DSC曲线构建玻璃化温度谱图的方法进行了改进,将橡胶态含量为0.5时的温度定义为聚合物的Tghr.采用改进Fevotte方法重新研究了PS的玻璃-橡胶态转变,PS的Tghr值比半比热外推法结果Tghc高约4K,当考察热处理时间和降温速率对聚合物玻璃-橡胶态转变的影响规律时,改进方法得到的结果与理论分析更为一致.聚合物的Tghr受热历史影响,随热处理条件改变,通过对不同降温速率冷却后样品滞后焓数据的拟合,推测PS样品的Tghr在377.5～390.9K范围内变化.     

Z-scheme structure SnS2–Au–CdS with excellent photocatalytic performance for simultaneous removal of Cr(VI) and methyl orange

Research on Chemical Intermediates - A highly efficient Z-scheme photocatalyst SnS2–Au–CdS was fabricated by photoreduction and thiourea medium deposition methods. It was found that Au...     

Application of Cationic Gemini Surfactant 12-3-12 and Methylene Blue in Simple Detection of DNA and Investigation of Interaction Mechanism

The present work aims to propose a new simple assay for deoxyribonucleic acids (DNA) by applying cationic Gemini surfactant 12-3-12 and cationic dye methylene blue (MB) as resonance light scattering (RLS) probes. The formation of MB-DNA-12-3-12 complex at pH 3.99 results in enhanced RLS signals at 369 nm, which is proportional to the concentration of DNA from 0 to 0.88 mg L^?1, with determination limit of 0.014 mg L^?1. Most foreign substances do not interfere in the detection and three synthetic samples are analyzed satisfactorily. transmission electron microscopy (TEM), FTIR, and ultraviolet spectra are used to investigate the interaction mechanism.     

Study of Characterization and Application on the Binding of Photochromic PAzoM26-b-PAA296 Diblock Copolymer with Bovine Serum Albumin by Spectroscopic Techniques

We have reported the synthesis and characterization of amphiphilic diblock copolymer {2-[4-(4-methoxy phenyl azo) phenoxy] hexyl acrylate co-acrylic acid} (PAzoM₂₆-b-PAA₂₉₆) and investigated the binding of this copolymer to bovine serum albumin (BSA) by TEM, ultraviolet-visible (UV-vis) absorption spectra, spectrofluorimetry, and synchronous fluorescence spectra techniques. The copolymer is photochromic and can self-assemble to form microspheres or vesicles, which could be potentially utilized in drug delivery system and the separation and purification of protein. We first present the inherent binding information using the fluorescence quenching technique, such as the quenching mechanism, binding constants, number of binding sites, thermodynamic parameters, and interaction driven forces. The binding distance and energy transfer efficiency were obtained based on Forster nonradiation energy transfer theory.