Total synthesis of miltirone

A concise synthesis of miltirone from 6-isopropyl-7-methoxy-1-tetralone is described, in which the naphthol was oxidized with Dess-Martin periodinane to yield miltirone in good yield. Published in Khimiya Prirodnykh Soedinenii, No. 6, pp. 541–543, November–December, 2006.     

苹果蠹蛾性信息素的合成研究进展

综述了用Wittig反应构建烯烃、炔化物还原、烯烃硼氢化、山梨酸衍生物格氏偶联、油脂的氧化-还原等方法,合成苹果蠹蛾性信息素(8E,10E-十二碳二烯-l-醇)的研究进展。并介绍了其部分类似物的合成方法。参考文献21篇。     

Ultrastructural and Morphological Effects in T-Lymphoblastic Leukemia CEM-SS Cells Following Treatment with Nordamnacanthal and Damnacanthal from Roots of Morinda elliptica

Background: Morinda elliptica (family Rubiaceae), locally known as ‘mengkudu kecil’, has been used by the Malays for medicinal purposes. Anthraquinones isolated from the roots of Morinda elliptica, namely nordamnacanthal and damnacanthal, have been widely reported to exhibit anticancer and antioxidant properties in various cancer models in vitro and in vivo. Aim: This study analyzed the morphological and ultrastructural effects of damnacanthal and nordamnacanthal on T-lymphoblastic leukemia CEM-SS cells. Method: Light microscopy, Giemsa staining, Wright’s staining, scanning electron microscopy, and transmission electron microscopy were carried out to determine apoptosis, necrosis, and ultrastructural changes that occurred within the cells. Results: The outcomes showed that these compounds induced cell death by apoptosis and necrosis, specifically at higher doses of 10 and 30 μg/mL. Condensation and fragmentation of the nuclear chromatin, which further separated into small, membrane-bound vesicles known as apoptotic bodies, were observed in the nuclei and cytoplasm. The plasma membranes and cytoskeletons also showed marked morphological changes upon treatment with damnacanthal and nordamnacanthal, indicating apoptosis. Conclusion: Therefore, we report that damnacanthal and nordamnacanthal exhibit anticancer properties by inducing apoptosis and necrosis in CEM-SS cells, and they have potential as a drug for the treatment of T-lymphoblastic leukemia.     

Fungicidal lipid-transfer peptide from <Emphasis Type="Italic">Daucus carota sativa</Emphasis> seeds

A non-specific lipid-transfer peptide (nsLTP) with fungicidal activity was isolated from Daucus carota sativa carrot seeds. Peptides were purified by a method including aqueous extraction, anion-exchange chromatography over CM-TSK-650M, and HPLC over a column of 250/8/4 Protein@Peptide C₁₈ using an acetonitrile gradient. The molecular weight of the peptide was determined as 9624 Da by mass spectrometry. The peptide was found to have fungicidal activity against the pathogenic fungus Verticillium dahliae. The partial N-terminal sequence, which was highly homologous to the N-terminal sequences of lipid-transfer peptides from seeds of rice, tobacco, and maize, was determined using Edman automated sequencing. __________ Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 371–373, July–August, 2007.     

Flat-twisted tubing: Novel column design for spiral high-speed counter-current chromatography

The original spiral tube assembly for high-speed counter-current chromatography (HSCCC) is further improved by a new tube configuration called “flat-twisted tubing” which was made by extruding the tube (1.6 mm I.D.) through a narrow slot followed by twisting along its axis forming about 1 cm twisted screw pitch. This modification interrupts the laminar flow of the mobile phase through the tube and continuously mixes the two phases through the column. The performance of this spiral tube assembly was tested by three types of two-phase solvent systems with different polarities each with a set of suitable test samples such as DNP-amino acids, dipeptides and proteins at the optimal elution modes. In general all these test samples yielded higher resolution with the lower mobile phase than the upper mobile phase. In the most hydrophobic two-phase solvent system composed of hexane–ethyl acetate–methanol–0.1 M hydrochloric acid (1:1:1:1, v/v/v/v), DNP–amino acids were separated with Rs-a (peak resolution based on the same column capacity adjusted for comparison) at 4.40 and 73% of stationary phase retention at a flow rate of 0.5 ml/min with the lower mobile phase. In the polar solvent system composed of 1-butanol–acetic acid–water (4:1:5, v/v/v), dipeptide samples were resolved with Rs-a at 4.06, compared to 2.79 with the cross-pressed tube assembly at 45% stationary phase retention, each at a flow rate of 1 ml/min. Finally in the aqueous–aqueous polymer phase systems composed of polyethylene glycol 1000 – dibasic potassium phosphate each 12.5% (w/w) in water, protein samples were resolved with Rs-a at 2.53 compared to 1.10 with the cross-pressed tube assembly at 52% of stationary phase retention, each at a flow rate of 1 ml/min. These results indicate that the present system substantially improves the partition efficiency with a satisfactory level of stationary phase retention by the lower mobile phase.     

新疆牛至挥发油化学成分的分析

气相色谱 -质谱技术对新疆牛至花和茎的挥发油化学成分进行分析研究。从牛至茎和花中分别鉴定了 4 2和 37种成分 ,占挥发油总量的 95 %以上 ,茎和花的挥发油化学成分相差很大     

Separation,preconcentration and speciation of chromium by solid phase extraction on immobilised ferron

A sensitive and simple method for determination of chromium species after separation and preconcentration by solid phase extraction (SPE) has been developed. For the determination of the total concentration of chromium in solution, Cr(VI) was efficiently reduced to Cr(III) by addition of hydroxylamine and Cr(III) was preconcentrated on a column of immobilised ferron on alumina. The adsorbed analyte was then eluted with 5?mL of hydrochloric acid and was determined by flame atomic absorption spectrometery. The speciation of chromium was affected by first passing the solution through an acidic alumina column which retained Cr(VI) and then Cr(III) was preconcentrated by immobilised ferron column and determined by FAAS. The concentration of Cr(VI) was determined from the difference of concentration of total chromium and Cr(III). The effect of pH, concentration of eluent, flow rate of sample and eluent solution, and foreign ions on the sorption of chromium (III) by immobilised ferron column was investigated. Under the optimised conditions the calibration curve was linear over the range of 2–400?µg?L^?1 for 1000?mL preconcentration volume. The detection limit was 0.32?µg?L^?1, the preconcentration factor was 400, and the relative standard deviation (%RSD) was 1.9% (at 10?µg?L^?1; n?=?7). The method was successfully applied to the determination of chromium species in water samples and total chromium in standard alloys.