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101.
Quantification of copepod gut content by differential length amplification quantitative PCR (dla-qPCR) 总被引:1,自引:0,他引:1
Christofer Troedsson Paolo Simonelli Verena Nägele Jens C. Nejstgaard Marc E. Frischer 《Marine Biology》2009,156(3):253-259
Quantification of feeding rates and selectivity of zooplankton is vital for understanding the mechanisms structuring marine
ecosystems. However, methodological limitations have made many of these studies difficult. Recently, molecular based methods
have demonstrated that DNA from prey species can be used to identify zooplankton gut contents, and further, quantitative gut
content estimates by quantitative PCR (qPCR) assays targeted to the 18S rRNA gene have been used to estimate feeding rates
in appendicularians and copepods. However, while standard single primer based qPCR assays were quantitative for the filter
feeding appendicularian Oikopleura dioica, feeding rates were consistently underestimated in the copepod Calanus finmarchicus. In this study, we test the hypothesis that prey DNA is rapidly digested after ingestion by copepods and describe a qPCR-based
assay, differential length amplification qPCR (dla-qPCR), to account for DNA digestion. The assay utilizes multiple primer
sets that amplify different sized fragments of the prey 18S rRNA gene and, based on the differential amplification of these
fragments, the degree of digestion is estimated and corrected for. Application of this approach to C. finmarchicus fed Rhodomonas marina significantly improved quantitative feeding estimates compared to standard qPCR. The development of dla-qPCR represents a
significant advancement towards a quantitative method for assessing in situ copepod feeding rates without involving cultivation-based
manipulation. 相似文献
102.
Improvements on Flood Alleviation in Germany: Lessons Learned from the Elbe Flood in August 2002 总被引:1,自引:0,他引:1
The increase in damage due to natural disasters is directly related to the number of people who live and work in hazardous
areas and continuously accumulate assets. Therefore, land use planning authorities have to manage effectively the establishment
and development of settlements in flood-prone areas in order to avoid the further increase of vulnerable assets. Germany faced
major destruction during the flood in August 2002 in the Elbe and Danube catchments, and many changes have been suggested
in the existing German water and planning regulations. This article presents some findings of a “Lessons Learned” study that
was carried out in the aftermath of the flood and discusses the following topics: 1) the establishment of comprehensive hazard
maps and flood protection concepts, 2) the harmonization of regulations of flood protection at the federal level, 3) the communication
of the flood hazard and awareness strategies, and 4) how damage potential can be minimized through measures of area precaution
such as resettlement and risk-adapted land use. Although attempts towards a coordinated and harmonized creation of flood hazard
maps and concepts have been made, there is still no uniform strategy at all planning levels and for all states (Lae
nder) of the Federal Republic of Germany. The development and communication of possible mitigation strategies for “unthinkable
extreme events” beyond the common safety level of a 100-year flood are needed. In order to establish a sustainable and integrated
flood risk management, interdisciplinary and catchment-based approaches are needed. 相似文献
103.
104.
Di Bernardino Annalisa Monti Paolo Leuzzi Giovanni Querzoli Giorgio 《Environmental Fluid Mechanics》2020,20(4):987-1005
Environmental Fluid Mechanics - We present results from water-channel experiments on neutrally-stable turbulent flows over staggered arrays of cubical obstacles modelling idealised urban canopies.... 相似文献
105.
Benito-Garzón Marta Fady Bruno Davi Hendrik Vizcaíno-Palomar Natalia Fernández-Manjarrés Juan 《Regional Environmental Change》2018,18(5):1427-1437
Regional Environmental Change - The adaptation of social-ecological systems such as managed forests depends largely on decisions taken by forest managers who must choose among a wide range of... 相似文献
106.
Ana L. De Toffoli Bruno H. Fumes 《Journal of environmental science and health. Part. B》2018,53(7):434-440
On-line in-tube solid phase microextraction (in-tube SPME) coupled to high performance liquid chromatography and tandem mass spectrometry (HPLC-MS/MS) was successfully applied to the determination of selected triazines in water samples. The method based on the employment of a packed column containing graphene oxide (GO) supported on aminopropyl silica (Si) showed that the extraction phase has a high potential for triazines extraction aiming to its physical-chemical properties including ultrahigh specific surface area, good mechanical and thermal stability and high fracture strength. Injection volume and loading time were both investigated and optimized. The method validation using Si-GO to extract and concentrate the analytes showed satisfactory results, good sensitivity, good linearity (0.2–4.0 µg L?1) and low detection limits (1.1–2.9 ng L?1). The high extraction efficiency was determined with enrichment factors ranging from 1.2–2.9 for the lowest level, 1.3–4.9 intermediate level and 1.2–3.0 highest level (n = 3). Although the analytes were not detected in the real samples evaluated, the method has demonstrated to be efficient through its application in the analysis of spiked triazines in ground and mineral water samples. 相似文献
107.
Md. Badiuzzaman Khan Mauro Masiol Caterina Bruno Alberto Pasqualetto Gian Maria Formenton Claudio Agostinelli Bruno Pavoni 《Environmental science and pollution research international》2018,25(32):31987-32000
A yearlong sampling campaign (2012–2013) was conducted in six major cities of the Veneto region to investigate the spatial-temporal trends and the factors affecting the polycyclic aromatic hydrocarbon (PAHs) variations and identify the local sources. Sixty samples per city were collected for analyses in every alternate month (April, June, August, October, December, and February): 10 samples per sampling site in 10 consecutive days of the months selected. Samples were ultrasonically extracted with acetonitrile and processed through high-performance liquid chromatography. Total Σ-PAH concentrations ranged from 0.19 to 70.4 ng m?3 with a mean concentration of 11.5 ng m?3. The mean benzo[a]pyrene (BaP) concentration reached 2.0 ng m?3, which is two-times higher than the limit set by the European Union. BaP contributed for 17.4% to the total concentration of PAHs, which showed the same pattern across the region with maxima during cold months and minima in the warm period. In this study, PAHs showed an inverse relationship with temperature, solar radiation, wind speed, and ozone. According to this study, biomass burning for household heating and cooking, followed by gaseous PAHs absorption on particles due to low atmospheric temperature, were the main reasons for increasing PAHs concentration in winter. Health risk, evaluated as lifetime lung cancer risk (LCR), showed a potential carcinogenic risk from the airborne BaPTEQ six-fold higher in the cold season than in the warm one. Diagnostic ratios and conditional probability functions were used to locate the sources, and results confirmed that local emission, overall domestic heating, and road transport exhausts were responsible for higher concentration rates of PAHs as well as of PM2.5. 相似文献
108.
109.
Distribution of PCDD/Fs and dioxin-like PCBs in sediment and plants from a contaminated salt marsh (Tejo estuary,Portugal) 总被引:1,自引:0,他引:1
Margarida Nunes Anaïs Vernisseau Philippe Marchand Bruno Le Bizec Fernando Ramos Miguel A. Pardal 《Environmental science and pollution research international》2014,21(4):2540-2549
Concentrations and profiles of 2,3,7,8-substituted polychlorinated dibenzo-p-dioxins, polychlorinated dibenzofurans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (dl-PCBs) were investigated in sediment and plants collected from a salt marsh in the Tejo estuary, Portugal. The highest PCDD/F and dl-PCB concentrations were detected in uncolonized sediments, averaging 325.25?±?57.55 pg g?1 dry weight (dw) and 8,146.33?±?2,142.14 pg g?1 dw, respectively. The plants Sarcocornia perennis and Halimione portulacoides growing in PCDD/F and dl-PCB contaminated sediments accumulated contaminants in roots, stems, and leaves. It was observed that PCDD/F and dl-PCB concentrations in roots were significantly lower in comparison with stems and leaves. In general, concentration of ΣPCDD/Fs and Σdl-PCBs in H. portulacoides tissues were found to be twofold higher than those in S. perennis, indicating a difference in the accumulation capability of both species. Furthermore, congener profiles changed between sediments and plant tissues, reflecting a selective accumulation of low chlorinated PCDD/Fs and non-ortho dl-PCBs in plants. 相似文献
110.
Paolo Littarru Luciano Vargiu 《Journal of the Air & Waste Management Association (1995)》2013,63(8):914-917
Abstract Fly ash from municipal solid waste incinerators (MSWIs) has been characterized in terms of polychlorinated dibenzyl-p-dioxin and polychlorinated dibenzofuran (PCDD/F) content. Increasing values of PCDD/Fs have been found to correlate with decreasing temperatures of sampling points in flue gas treatment lines of the plants, confirming other researchers’ findings about temperature as the major controlling parameter for the PCDD/F formation. Measured PCDD/F ratios show that de novo synthesis is the dominant formation mechanism. The increasing trends of particulate-bound PCDD/Fs can be explained not only through the dominant de novo synthesis process but also considering the adsorption of gaseous PCDD/Fs on fly ash deposits, even outside the typical de novo synthesis temperature ranges. The effective role of a post-combustor unit, imposed by Italian law to destroy PCDD/Fs, also needs to be carefully reconsidered. 相似文献