Comparison of gas chromatography and immunoassay methods in measuring the distribution of dieldrin in rainbow trout tissues.

Studies have been conducted to determine the distribution of dieldrin in various tissues of rainbow trout when exposed to several dieldrin concentrations. Medium sized fish with an average weight and length of 195.4 +/- 30.5 g and 25.7 +/- 1.4 cm, respectively, were placed in groups of 6 in 300 L tanks containing purified and aerated water and maintained at 10 degrees C. Following an acclimatization period of 10 days, each group of fish was exposed to one of four dieldrin concentrations ranging from 50 to 80 ppb. After 24 hours, the fish were taken out of the tanks and sacrificed. The brain, gills, liver, muscles and skin were collected from each fish. Dieldrin was extracted from each tissue using SPE techniques and analyzed by both gas chromatography (GC) and enzyme linked immunosorbent assay (ELISA). Results of analyses by the two techniques were highly correlated. The results also showed that liver and skin tissues had the highest level of dieldrin residues. In comparing the means of the six fish samples, it was found that liver or skin contained about 1.5-fold the level in brain, about 4.0 fold the level in muscles and about 6.5 fold the level in gills. Immunoassay proved to be as reliable an analytical tool as gas chromatography in this case.     

Cleaner tanning practices for tannery pollution abatement: Role of enzymes in eco-friendly vegetable tanning

Concern about pollution related problems in the global scenario are persuading all the processing industries to adopt cleaner manufacturing practices. Thus, the leather industry is also under pressure to look for effective alternative tanning materials for chromium. Natural products like vegetable tannins are regaining importance. However, there are limitations in the use of vegetable tanning materials because of its high organic load in the effluent, which are difficult to degrade leading to high chemical oxygen demand (COD). Moreover, conventional vegetable tanning process requires partial pickling that involves the use of sodium chloride, to suppress osmotic swelling. This results in very high amount of total dissolved solid (TDS) content in wastewaters. In this investigation, an attempt has been made to design an eco-friendly vegetable tanning process combining pickle-free tanning and application of proteolytic enzymes to improve the exhaustion of vegetable tannins. Such an approach has resulted in more than 95% tannin exhaustion in the case of the experimental process, an increase of 10% compared with the conventional vegetable tanning process. The tanned leathers showed slight improvement in hydrothermal stability. Physical and tactile evaluation of experimental leathers has been better than conventionally tanned leathers. Surface colour values illustrated negligible variation in colour and shade between control and experimental leathers. The resultant leather showed opened up, split compact fibre structure that has been well coated, indicating that the enzyme assisted tanning process did not bring about any major change or destruction on the fibre structure of the leathers. The optimized system has been field tested in a commercial tannery. The results showed that the enzyme assisted tanning process is efficient in terms of improved quality of leather and also led to reduction in total solids (TS), chlorides and COD loads. The enzyme assisted tanning system presented appears to be a viable option for combating pollution arising from the conventional vegetable tanning system.     

Determination of organochlorine and organophosphate pesticide residues in fruits, vegetables and sediments.

A method is described for the determination of organochlorine and organophosphate pesticide residues in fruits, vegetables and sediments. The concentrated solvent extract was sealed in a polymeric membrane tube, dialysed in cyclohexane and the solvent replaced with hexane. The organophosphates were analysed on a specific thermionic detector without further clean-up. For the organochlorine pesticides the extract was eluted through 3 g of alumina and analysed on GC/ECD. The clean-up for sediment extract was carried out on a 10 g alumina column with 100 mL hexane containing 5% acetone and the eluate was concentrated to 5 mL. The detection limit for organophosphates on a 40 g sample and a final volume of 10 mL was on the average 0.01 mg/kg. The detection limit for organochlorine pesticides, with the final volume of 25 mL, was 0.005 mg/kg for all pesticides except for p,p'-DDT and endosulfan sulphate, which was 0.01 mg/kg. The detection limit for organochlorine pesticides in sediment, with the final volume of 2 mL, was less than 1 microgram/kg and for organophosphate pesticides less than 10 micrograms/kg when the final volume was made to 0.5 mL. At the detection limits the method produced a very high coefficient of variation for both organochlorine and organophosphate pesticides.     

Metabolic fate of dichloromethyl‐0,0‐diphenyl phosphonate (I) and dichloromethyl‐0,0‐di‐(p‐nitrophenyl) phosphonate (II) in/on rice plants∗

Abstract

Metabolic fate of two dichloromethyl diaryl phosphonates (³²P labelled) in/on rice plants was investigated. The test compounds were found to be less persistent on the surface of rice leaves with half lives 7.4 and 6.7 days respectively. Main degradation product from both the phosphonates were dichloromethyl phosphonic acid with trace of dichloromethyl‐O‐aryl phosphonate as a transitory intermediate product.     

Higher soybean production using honeybee and wild pollinators, a sustainable alternative to pesticides and autopollination

This is the first report showing that using honeybee (Apis mellifera) and wild pollinators complementary pollination can enhance soybean productivity (Glycine max). Current industrial production of soybean involves autopollination and high loads of pesticides. Therefore, growers have neglected possible biotic pollination despite suggestions that soybean benefit from insect pollinators. Reports advocating possible biotic pollination are based on experiments where bees are caged with flowering plants and the absence of pesticides, thus not in field conditions. Therefore, here we compared in field conditions soybean yield produced (1) independently of biotic pollinators, (2) with wild pollinators and (3) with honeybee colonies. Results showed an increase of +6.34 % of soybean yield in areas where wild pollinators had free access to flowers. The introduction of honeybee colonies further raised the yield of +18.09 %. Our findings therefore show that, though soybean is autogamous, allowing pollination by wild pollinators leads to higher yields. Moreover, adding honeybee mitigates pollination deficits and improves yield compared to current practices.