Novel experimental strategy for high resolution AFM imaging of membrane-associated bacterial toxins

Bacterial pore-forming toxins （PFTs） are essential virulence factors of many human pathogens. Knowl- edge of their structure within the membrane is critical for an understanding of their function in pathogenesis and for the development of useful therapy. Atomic force microscopy （AFM） has often been employed to structurally interrogate many membrane proteins, including PFTs, owing to its ability to produce sub-nanometer resolution images of samples under aqueous solution. However, an absolute prerequisite for AFM studies is that the samples are single-layered and closely-packed, which is frequently challenging with PFTs. Here, using the prototypical member of the cholesterol-dependent cytolysin family of PFTs, perfringolysin O （PFO）, as a test sample, we have developed a simple, highly robust method that routinely produces clean, closely-packed samples across the entire specimen surface. In this approach, we first use a small Teflon well to prepare the supported lipid bilayer, remove the sample from the well, and then directly apply the proteins to the bilayer. For reasons that are not clear, bilayer preparation in the Teflon well is essential. We anticipate that this simple method will prove widely useful for the preparation of similar samples, and thereby enable AFM imaging of the greatest range of bacterial PFTs to the highest possible resolution.     

Effect of nozzle orifice diameter on diesel spray tip penetration according to various spray models for CFD simulation with widely varying back pressure

CFD simulations of spray tip penetration with the standard KIVA3V, ‘original gas jet’ and ‘Normal gas jet profile with breakup length formula’ (NGJBL) spray models were performed to investigate the effects of nozzle orifice size and ambient gas density combinations on the spray penetration. The accuracy of the CFD simulation results was estimated by comparing them with available experimental data. The ambient gas density was varied in 12 kg/m³ intervals from 12 to 69 kg/m³ for each nozzle orifice diameter. The nozzle orifice diameters used were 119, 140, 183 and 206 mm. A total of 20 cases in the CFD simulations were considered with combinations of the 4 nozzle orifice diameters and 5 ambient gas densities. CFD simulations with the NGJBL spray model were more accurate than those with either the standard KIVA3V or gas jet spray models as the nozzle orifice diameter and ambient gas density was increased. The NGJBL and original gas jet model is more effective in predicting the spray tip penetration near the nozzle tip region.     

Vibration reduction of H/Shaft using an electromagnetic damper with mode change

The objective of this study is to develop a damper that can reduce the amplitude of vibration in various frequency ranges. Previous H/Shaft vibration reduction methods work in a passive way. A dynamic damper reduces the amplitude of vibration at its first mode, but vibration still appears at the second mode. A mass damper or hollow shaft can shift the natural frequency to a lower or higher region. The fixed operating frequency prevents vibration from being reduced outside the operating frequency range. The proposed damper uses electromagnets as either masses or actuators to change the damper mode between dynamic damper mode and mass damper mode. The electromagnetic damper (EMD) can change its mode to respond to the vibration excitation at both low and high frequencies. The vibration reduction performance was evaluated by FRF tests in laboratory and vehicle conditions. The results were compared with those of a dynamic damper and indicate that the amplitude of vibration is reduced by 95.6 % when the EMD is implemented on an H/Shaft, whereas only 61.9 % vibration reduction is achieved by the dynamic damper.     

自行车流视频识别判断方法研究

考虑算法的实时性,交通流个体识别往往采用轮廓特征来描述。由于自行车与行人的很多轮廓特征值相近,在复杂城市交通环境中,从慢行交通流中准确地识别出自行车是目前采用视频检测交通流的难点之一。通过现场视频检测获取足够的样本外轮廓数据,分析检测目标轮廓的高、宽、高宽比、面积等典型轮廓特征,发现目标轮廓高宽比特征能较好地识别出行人、自行车等慢行交通,并给出合适的取值范围。研究表明自行车高宽比能够较好地成为视频识别的依据,为自行车视频识别提供数据。     

甘泉铁路区间通过能力研究

阐述甘泉铁路车站分布和运量发展情况及货运量增加后区间通过能力不足的问题,介绍甘泉铁路行车组织和区间通过能力计算方法,计算各区间的通过能力,结合近期货物运量500万t、700万t、1000万t三种情况,计算需求通过能力,并给出提高通过能力的具体方案,为下一步货运量的增加做准备。     

使用有限元分析以改善重载运输车轴

分析了北美铁路货运发生的车轴故障,介绍了使用有限元对F级和K级车轴进行应力分析计算,研究车轴尺寸变化对应力的影响,并提出了改善重载车轴设计的建议.     

CFD Analysis of Ship-to-Ship Hydrodynamic Interaction

A numerical study of ship-to-ship interaction forces is performed using a commercial CFD code, and the results are compared with experimental data and with the results of a panel method analysis. Two ship models have been used in the interaction forces analysis: a tug and a tanker, advancing parallel to each other with different lateral distances and two different values of the fluid depth. Computations are carried out with four different flow models: inviscid and viscous flow with the free surface modeled as a rigid wall and inviscid and viscous flow with the deformable free surface. A fair agreement was obtained with available experimental data and results obtained by panel method. The influence of viscosity in the computations is found to be comparatively weak, while the wavemaking effects may be important, at small magnitude of the horizontal clearance.     

Are we ready to embrace connected and self-driving vehicles? A case study of Texans

While connected, highly automated, and autonomous vehicles (CAVs) will eventually hit the roads, their success and market penetration rates depend largely on public opinions regarding benefits, concerns, and adoption of these technologies. Additionally, the introduction of these technologies is accompanied by uncertainties in their effects on the carsharing market and land use patterns, and raises the need for tolling policies to appease the travel demand induced due to the increased convenience. To these ends, this study surveyed 1088 respondents across Texas to understand their opinions about smart vehicle technologies and related decisions. The key summary statistics indicate that Texans are willing to pay (WTP) $2910, $4607, $7589, and $127 for Level 2, Level 3, and Level 4 automation and connectivity, respectively, on average. Moreover, affordability and equipment failure are Texans’ top two concerns regarding AVs. This study also estimates interval regression and ordered probit models to understand the multivariate correlation between explanatory variables, such as demographics, built-environment attributes, travel patterns, and crash histories, and response variables, including willingness to pay for CAV technologies, adoption rates of shared AVs at different pricing points, home location shift decisions, adoption timing of automation technologies, and opinions about various tolling policies. The practically significant relationships indicate that more experienced licensed drivers and older people associate lower WTP values with all new vehicle technologies. Such parameter estimates help not only in forecasting long-term adoption of CAV technologies, but also help transportation planners in understanding the characteristics of regions with high or low future-year CAV adoption levels, and subsequently, develop smart strategies in respective regions.