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预算编制执行中存在的问题及对策

1部门预算编制中存在的问题(1)尚未建立科学的标准体系和管理体系。一方面,由于国家、省、市出台的行政事业单位开支标准制度相对滞后,执行中“超标”现象普遍存在,增加了建立科学合理的预算定额标准体系的可操作难度。另一方面,由于资源调查基础数据不够准确、真实,不能正确反     

莱芜市莱城区全力搞好矿产资源管理工作

0引言莱芜市莱城区位于鲁中地区,辖15个乡镇(办事处),865个行政村(居委会),总面积1 853 km2,人口94．2万。境内已发现54种矿产,其中已探明储量的矿产有22种,以煤、铁资源最为丰富。铁矿以富矿为主,资源储量约4．5亿t;煤矿资源储量约8亿t。区内矿山企业160处,总生产能力1．33×107t/a。由于区内地形复杂,矿山企业分散,给矿产资源的管理带来很大难度。     

BAC end sequencing of Pacific white shrimp <Emphasis Type="Italic">Litopenaeus vannamei</Emphasis>: a glimpse into the genome of Penaeid shrimp

Little is known about the genome of Pacific white shrimp (Litopenaeus vannamei). To address this, we conducted BAC (bacterial artificial chromosome) end sequencing of L. vannamei. We selected and sequenced 7 812 BAC clones from the BAC library LvHE from the two ends of the inserts by Sanger sequencing. After trimming and quality filtering, 11 279 BAC end sequences (BESs) including 4 609 paired- ends BESs were obtained. The total length of the BESs was 4 340 753 bp, representing 0.18% of the L. vannamei haploid genome. The lengths of the BESs ranged from 100 bp to 660 bp with an average length of 385 bp. Analysis of the BESs indicated that the L. vannamei genome is AT-rich and that the primary repeats patterns were simple sequence repeats (SSRs) and low complexity sequences. Dinucleotide and hexanucleotide repeats were the most common SSR types in the BESs. The most abundant transposable element was gypsy, which may contribute to the generation of the large genome size of L. vannamei. We successfully annotated 4 519 BESs by BLAST searching, including genes involved in immunity and sex determination. Our results provide an important resource for functional gene studies, map construction and integration, and complete genome assembly for this species.     

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Taxonomic and genetic status of lancelet in Weihai coastal waters based on mitochondrial DNA sequence

Lancelets (subphylum Cephalochordata) are a transitional species between invertebrates and vertebrates. They are currently listed in the Second Order of Protected Animals in China. Lancelets were first documented in the waters around the city of Weihai (Shandong, China) in 2002. However, little is known about the phylogeny of this population. We analyzed the sequences of cytochrome b (Cyt b) and cytochrome oxidase c subunit I (CO I) genes from samples collected from coastal waters in the cities of Weihai and Qingdao (~150 km to the south). We analyzed 176 sequences, of which 150 were novel sequences and 26 were obtained from GenBank. Our results suggest that (1) lancelets in the two cities belong to the species Branchiostoma japonicus and have a high level of genetic diversity; (2) there is a high level of gene flow and low level of genetic differentiation between lancelets from the two cities; (3) demographic expansion occurred an estimated 1.1 million years (Ma) ago (mid Pleistocene) for lancelets in Weihai-Qingdao; and (4) the divergence between B. belcheri and B. japonicus was estimated at between 37.75 Ma (early Oligocene)-46.5 Ma (late Eocene).     

Industrial structural changes in Hong Kong, China under one country, two systems framework

Hong Kong has undergone substantial economic transformations and developed into a sophisticated business and financial center in the Asia-Pacific region,since the return of sovereignty to China as a Special Administrative Region under the One Country Two Systems (OCTS) in 1997.This paper discusses and analyzes the industrial structural changes of Hong Kong in recent decades as well as its future challenges and opportunities.The data and finding reveal that even though Hong Kong will face fierce competition from the Mainland’s cities as the rise of China,the important role as a bridge between China and the rest of the world will brace Hong Kong itself under the OCTS for developing into a service hub for business and trade in the Asia-Pacific region.     

Molecular identification based on ITS sequences for Kappaphycus and Eucheuma cultivated in China

The systematic classification of the Eucheumatoideae is difficult because of their variable morphology and interpretation of reproductive structures. Kappaphycus and Eucheuma specimens cultivated on the Hainan and Fujian coast of China were introduced from Vietnam, the Philippines and Indonesia. Combined with morphological characteristics, all Kappaphycus and Eucheuma cultivated strains were identified by internal transcribed spacer (ITS) sequences. The phylogenetic tree was constructed using neighbor-joining and maximum likelihood methods. The results indicate that different ITS sequence lengths occurred in the different genera and species. An obvious difference in morphology could be found in the protuberance shape between Kappaphycus and Eucheuma. The protuberance in Eucheuma was thorn-like and in Kappaphycus was wartlike or papillate. Their ITS sequence lengths differed significantly in nucleotide variation rates up to 58.55%-63.90%. All nucleotide variations occurred in the ITS1 and ITS2 regions except for five nucleotide transversions in the 5.8S rDNA region. In addition, the difference was at the branches among congeneric species. Kappaphycus sp. had branches with small buds, while K. alvarezii did not have such a feature. The nucleotide variation rates varied from 7.02% to 7.48% among species; within the same species of the clades it was <1.20%. Eucheumatoideae algae cultivated in China consisted of three clades, K. alvarezii, Kappaphycus sp., and E. denticulatum. The results indicate that ITS sequence analysis was an effective way for identification of interspecies and intraspecies phylogenetic relationships and might provide a clue for molecular identification of algal Eucheumatoideae.