Heavy metal analyses in the marine environment - approaches to quality control

The validity of analyses of elements in the femto- and picomolar ranges in waters and sediments can be assessed on the basis of several criteria: an evaluation of the chemical technique; the use of primary standards and intercalibration exercises; and chemical and geochemical coherence of the results. Herein are reported analyses of ruthenium, platinum, palladium, rhenium, iridium and gold in oceanic samples. The gold concentrations in surface seawaters, at least 25 times less than those previously reported, are in accord with the concentrations of their periodic table neighbors as well as with crustal and sedimentary contents. The remarkable concentration of rhenium in seawater, in relation to its very low crustal abundance, can be explained by the inertness of the perrhenate ion.     

A modified linear analysis of a wave-energy conversion buoy

The analysis of a pneumatic-type wave-energy conversion buoy is developed assuming independence of the buoy heaving motion and the motion of the water column within the center pipe. Results of the analysis are then compared with experimental data in a study of the relative air velocity within the turbine passage. The results compare very well. The effect of the variation of the center pipe length is found to be significant for periods about the surge chamber resonance but is negligible in the neighborhood of the heaving resonance period. Further, the theory is applied to a prototype buoy study of the U.S. Coast Guard, and a dimensionless design curve is developed from the results of the prototype analysis.     

Preservation of enzymic activity in marine plankton by low-temperature freezing

The measurement of enzymic activity in plankton communities is useful in the study of marine ecosystems. Such measurements can lead to a clearer understanding of the biological transformations in plankton communities at a particular time. However, the assays are somewhat time-consuming. To facilitate analysis of large numbers of samples, we have developed a method of quick-freezing of whole cells and of cell-free extracts of the diatom, Skeletonema costatum, followed by storage at ?60°C for up to one week. No loss in either the electron transport system (ETS) or the glutamate dehydrogenase (GDH) activity occurs. Similar conditions of storage can be used for the preservation of ETS activity in the marine copepod Calanus pacificus. Also, no measurable loss in either the GDH or the ETS activity is detectable after the quick-frozen whole cells of S. costatum have been kept frozen for over a year.     

The hydrogen sulphide boundary in the Black Sea in the 1920s and 1980s

Maps of the boundary topography for anaerobic waters in the Black Sea are plotted for the summers of the 1920s and 1980s. The mean depths of the H₂S boundary for this period are determined. It is shown that the variation in the distribution and average location of the hydrogen sulphide zone has remained constant for the last 60 years.Translated by Mikhail M. Trufanov.