In order to thoroughly investigate the diversity of glacier microorganisms, four DNA extraction methods with different lysis patterns
were tested and two screened methods (the Bosshard-Bano method and the Zhou method) were optimized for the most effective
form of the filter membrane (cut vs. uncut), the DNA extraction method, and the precipitation method. The two optimized
methods were then compared with the commercial Mo-Bio DNA extraction kit, and the results showed that the kit was generally
suitable for extraction of microorganism DNA from glacier surface snow. Procedurally, it was found that a modified Bosshard-
Bano method (i.e., cutting the filter membrane into pieces, using a specific lysis pattern [lysozyme (5 mg/mL)-protease K
(1 mg/mL)-CTAB (1%)-SDS (1%)], performing the extraction only once by chloroform-isoamyl alcohol (24:1), and conducting
DNA precipitation by pure ethanol) was also an effective and less expensive method for extraction of microorganism DNA from
glacier surface snow. 相似文献
The Laohugou Glacier No. 12 is the largest valley glacier in Qilian Mountains, which is located in northern Qinghai-Tibet Plateau. Movement is the basic characteristic of glaciers, and is also an important distinction from other terrestrial natural ice. Glacier changes not only reflect climate change, but also play an important role in humanity society. In the arid regions of western China, glaciers are becoming an important water source. We use the GPS receiver (South-Lingrui S82) as data platform with the aid of RTK measurement technology to observe the surface velocity of Laohugou Glacier No. 12. Surface velocity data shows that the maximum value appears at an altitude of 4,750–4,850 m during the period of 2008–2009. During this period, the west branch surface velocity reached 32.6 m per year at an altitude near 4,830 m, the east branch surface velocity reached 32.4 m per year at the altitude near 4,770 m. Comparing the surface velocity data during 2008–2009 with observation results in 1959, the glacier velocity slowed down about 11%. 相似文献
This paper attempts to explore the temporal and spatial nature of the marginal revenue of land, total factor productivity (TFP) change and its three components: technical change (TC), technical efficiency change (TEC) and scale efficiency change (SEC) as seen in Chinese agricultural production from 1995 to 1999. Based on county-level data, the study utilized both stochastic frontier and mapping analyses methods. The results show that growth in the marginal revenue of land was diverse across various regions, where most gain occurred in eastern coastal zone, while loss was in Northwest and North China. China has experienced moderate decreases in annual TFP change (-0.26%) with considerable regional variations. Specifically, the administrative intervention in grain production and the deterioration of the agricultural technology diffusion system led to a moderate drop in annual TFP change. County-level mapping analyses took into account interregional variances in TFP and its components. Regarding components of TFP, TEC differences explain the majority of regional dispersions in TFP. As developed areas in China, the Huang-Huai-Hai region and the Beijing-Tianjin-Tangshan economic zone face the challenges of land conversion and grain security amidst the process of urbanization. 相似文献
Geotechnical and Geological Engineering - The stability monitoring of surrounding rock in layered roadway is an important method to ensure the safety of deep mining coal mine. In view of the actual... 相似文献
Hydraulic fracturing is an essential technology for the development of unconventional resources such as tight gas. The evaluation of the fracture performance and productivity is important for the design of fracturing operations. However, the traditional dimensionless fracture conductivity is too simple to be applied in real fracturing operations. In this work, we proposed a new model of dimensionless fracture conductivity (FCD), which considers the irregular fracture geometry, proppant position and concentration. It was based on the numerical study of the multistage hydraulic fracturing and production in a tight gas horizontal well of the North German Basin. A self-developed full 3D hydraulic fracturing model, FLAC3Dplus, was combined with a sensitive/reliability analysis and robust design optimization tool optiSLang and reservoir simulator TMVOCMP to achieve an automatic history matching as well as simulation of the gas production. With this tool chain, the four fracturing stages were history matched. The simulation results show that all four fractures have different geometry and proppant distribution, which is mainly due to different stress states and injection schedule. The position and concentration of the proppant play important roles for the later production, which is not considered in the traditional dimensionless fracture conductivity FCD. In comparison, the newly proposed formulation of FCD could predict the productivity more accurately and is better for the posttreatment evaluation.
The study of Precambrian rifts, such as those found in the Tarim craton, is of great interest in the field of deep petroleum exploration as the source rocks of the rifts are depositional deep lacustrine facies. In this study, two buried rifts (Manjiaer and Wushi rifts) that may exist in the Tarim craton are identified based on the seismic profiles. Through the analysis of the outcrops and stratigraphic columns of the identified rifts, two types of rifts are classified and their evolution is divided into different stages. The evolution of the Manjiaer rift, which experiences frequent volcanic activity, can be roughly divided into three rift periods. Meanwhile, the evolution of the Wushi rift can be simply divided into two rift periods during the Nanhuan–Sinian period. A noticeably stronger activity of the Manjiaer rift in comparison with that of the Wushi rift was noted. The paleogeography of the Proterozoic Tarim craton was also reconstructed, and the prospect for deep petroleum exploration in the Tarim craton is proposed. 相似文献