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1.
Microsatellite DNA in peach (Prunus persica L. Batsch) and its use in fingerprinting and testing the genetic origin of cultivars. 总被引:11,自引:0,他引:11
R Testolin T Marrazzo G Cipriani R Quarta I Verde M T Dettori M Pancaldi S Sansavini 《Génome》2000,43(3):512-520
We isolated and sequenced 26 microsatellites from two genomic libraries of peach cultivar 'Redhaven', enriched for AC/GT and AG/CT repeats, respectively. For 17 of these microsatellites, it was possible to demonstrate Mendelian inheritance. Microsatellite polymorphism was assayed in 50 peach and nectarine cultivars. Of the 1300 PCRs carried out, all but two produced amplified products of the expected size. All microsatellites were polymorphic, showing 2-8 alleles per locus. Heterozygosity ranged from 0.04-0.74 (mean 0.47); the discrimination power (PD) ranged from 0.04-0.84 (mean 0.60). Cultivar heterozygosity varied greatly, with one cultivar ('Independence') being homozygous at all loci. The set of microsatellites discriminated all cultivars investigated, except several sport mutations, i.e., 'Dixitime' vs. 'Springcrest', 'Compact Redhaven' vs. 'Redhaven', and two pairs of cultivars, 'Venus' vs. 'Orion' and 'Elegant Lady' vs. 'Rome Star', whose pedigrees are controversial. We were able to analyze the paternity of several cultivars. In most cases, the parenthood was confirmed. The comparison of three long-living 'Redhaven' accessions supplied by different repositories did not provide any evidence of somatic instability of microsatellites. Hence, microsatellites, ranked according to their information content, are recommended as markers of choice for peach fingerprinting and suggestions are provided for interpreting band profiles and the correct sizing of alleles. 相似文献
2.
Anna Maria Greco Pasquale Gambardella Raffaella Sticchi Diego D'aponte Pietro De Franciscis 《Chronobiology international》1988,5(3):217-225
Circadian rhythms of serotonin (5HT), its precursors tryptophan (TP) and 5-hydroxy-tryptophan (5HTP) and its acid catabolite 5-hydroxy-indoleacetic acid (5HIAA), were determined in the hypothalamus of control rats and rats which had been treated continuously with subcutaneous imipramine (10 mg/kg/day) for 2 weeks.
Rats were individually housed and entrained to LD12:12. Controls showed the 5HT and TP peaks in the light and dark periods respectively, as reported in the literature, but no inverted correlation (antiphase) between SHT and 5HIAA rhythms.
Imipramine significantly modified circadian rhythm characteristics: the 5HT acrophase was advanced, that of TP and 5HIAA was delayed. Imipramine also significantly increased hypothalamic SHT and TP concentrations. 相似文献
Rats were individually housed and entrained to LD12:12. Controls showed the 5HT and TP peaks in the light and dark periods respectively, as reported in the literature, but no inverted correlation (antiphase) between SHT and 5HIAA rhythms.
Imipramine significantly modified circadian rhythm characteristics: the 5HT acrophase was advanced, that of TP and 5HIAA was delayed. Imipramine also significantly increased hypothalamic SHT and TP concentrations. 相似文献
3.
Reduced mechanical activity of perfused rat heart following morphine or enkephalin peptides administration 总被引:1,自引:0,他引:1
Carlo Clô Claudio Muscari Benedetta Tantini Carla Pignatti Pasquale Bernardi Carlo Ventura 《Life sciences》1985,37(14):1327-1333
In the isolated and perfused rat heart, the addition of morphine, methionine-enkephalin or leucine-enkephalin to the coronary perfusate, significantly reduces the mechanical activity by negatively affecting both the heart rate and the developed tension. These effects are dose dependent and maximally evident with leucine-enkephalin. Furthermore all the opioids strongly reduce the activity of isoproterenol-stimulated hearts. The suggestion is made that opioid peptides directly influence the cardiac mechanical activity possibly by interacting with membrane-receptor systems. 相似文献
4.
In the Venice Lagoon (Italy), about 106 t (wet weight) of Ulva rigida biomass are produced annually as one of the major results of eutrophication. Harvests have been initiated to reduce negative
impacts of this biomass, however, due to the high costs of such effort, only 40 000 t yr-1 are currently being collected. At the moment, biotransformation into compost seems to be the only feasible technology for
utilising large quantities of Ulva biomass. We describe and discuss here a successful composting strategy together with the chemico-physical and microbiological
characteristics of the resultant composts. Our composting experiments were conducted at a scale of 20 t. The composting technology
utilises large proportions (70–90%) of Ulva biomass and results in a valuable, high-quality end product (compost and compost-based products). This process and the resulting
products represents a relatively simple way of utilising the Ulva biomass produced annually in the Venice Lagoon. 相似文献
5.
6.
Pasquale Petrilli Pietro Pucci Anna Maria Garzillo Giovanni Sannia Dr. Gennaro Marino 《Molecular and cellular biochemistry》1981,35(2):121-128
Summary Reactivity of sulphydryl groups of cytosolic and mitochondrial aspartate aminotransferases from ox heart has been studied.
A total of 5 and 7 cysteine residues per monomer are present in cAATo and mAATo, respectively. In native conditions only a
single sulphydryl group can be titrated by Nbs2 while the catalytic activity remains unchanged, however in the mitochondrial isozyme the reactivity depends on the functional
state of the enzyme. Reactivity toward NEM reveals the existence of a syncatalytic sulphydryl group in the cytosolic isozyme.
Titration of cAATo with pMB at pH 8 and pH 5 confirms the existence of two exposed sulphydryl groups with a different reactivity.
The results compared with those reported on the corresponding isozymes from pig and chicken heart show that syncatalytic sulphydryl
groups are of general occurrence in these enzymes. 相似文献
7.
Henry R. Black Samuel O. Thier Philip F. Felig James F. Jekel Joseph Belsky James L. Bernene Everett Cooper Vincent A. De Luca Jr. Martin H. Floch Marvin Garrell Pasquale E. Perillie Robert L. Piscatelli George F. Thornton 《The Yale journal of biology and medicine》1977,50(6):637-644
In July, 1975, the Departments of Internal Medicine at the Yale University School of Medicine and eight community hospitals in southern and western Connecticut formed the Yale Affiliated Hospital Program (YAHP) in Internal Medicine. The YAHP provides a planned and focused program of continuing education for medical staff and housestaff at the affiliated hospitals. Six formats for the over 1,000 rounds, lectures, and conferences given annually are used. The members of the YAHP also cooperate in housestaff and faculty recruiting, evaluation of quality of care and evaluation of the process of continuing medical education itself. This report summarizes the organization, goals and future plans of the YAHP. 相似文献
8.
9.
Eph receptor signalling casts a wide net on cell behaviour 总被引:11,自引:0,他引:11
Pasquale EB 《Nature reviews. Molecular cell biology》2005,6(6):462-475
Eph receptor tyrosine kinases mould the behaviour of many cell types by binding membrane-anchored ligands, ephrins, at sites of cell-cell contact. Eph signals affect both of the contacting cells and can produce diverse biological responses. New models explain how quantitative variations in the densities and signalling abilities of Eph receptors and ephrins could account for the different effects that are elicited on axon guidance, cell adhesion and cell migration during development, homeostasis and disease. 相似文献
10.