全文获取类型
收费全文 | 534篇 |
免费 | 38篇 |
学科分类
生物科学 | 572篇 |
出版年
2024年 | 2篇 |
2023年 | 3篇 |
2022年 | 8篇 |
2021年 | 5篇 |
2020年 | 7篇 |
2019年 | 4篇 |
2018年 | 11篇 |
2017年 | 7篇 |
2016年 | 11篇 |
2015年 | 15篇 |
2014年 | 22篇 |
2013年 | 37篇 |
2012年 | 28篇 |
2011年 | 33篇 |
2010年 | 25篇 |
2009年 | 21篇 |
2008年 | 31篇 |
2007年 | 39篇 |
2006年 | 26篇 |
2005年 | 36篇 |
2004年 | 30篇 |
2003年 | 32篇 |
2002年 | 20篇 |
2001年 | 10篇 |
2000年 | 8篇 |
1999年 | 15篇 |
1998年 | 8篇 |
1997年 | 3篇 |
1996年 | 8篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1993年 | 5篇 |
1992年 | 8篇 |
1991年 | 8篇 |
1990年 | 4篇 |
1989年 | 5篇 |
1988年 | 4篇 |
1987年 | 3篇 |
1986年 | 1篇 |
1984年 | 4篇 |
1983年 | 5篇 |
1982年 | 2篇 |
1979年 | 2篇 |
1978年 | 3篇 |
1974年 | 3篇 |
1972年 | 2篇 |
1971年 | 1篇 |
1970年 | 2篇 |
1969年 | 1篇 |
1965年 | 1篇 |
排序方式: 共有572条查询结果,搜索用时 15 毫秒
1.
Y Hamano H Kodama M Yanagisawa Y Haraguchi M Mori S Yokota 《The journal of histochemistry and cytochemistry》1988,36(1):29-35
We investigated light and electron microscopic localization of ornithine transcarbamylase (OTC) in rat intestinal mucosa. In the immunoblotting assay of OTC-related protein, a single protein band with a molecular weight of about 36,500 is observed in extracts of liver and small intestinal mucosa but is not observed in those of stomach and large intestine. For light microscopy, tissue slices of the digestive system were embedded in Epon and stained by using anti-bovine OTC rabbit IgG and the immunoenzyme technique. For electron microscopy, slices of these and the liver tissues were embedded in Lowicryl K4M and stained by the protein A-gold technique. By light microscopy, the absorptive epithelial cells of duodenum, jejunum, and ileum stained positively for OTC, but stomach, large intestine, rectum, and propria mucosa of small intestine were not stained. Electron microscopy showed that gold particles representing the antigenic sites for OTC were confined to the mitochondrial matrix of hepatocytes and small intestinal epithelial cells. However, the enzyme was detected in mitochondria of neither liver endothelial cells, submucosal cells of small intestine, nor large intestinal epithelial cells. Labeling density of mitochondria in the absorptive epithelial cells of duodenum, jejunum, and ileum was about half of that in liver cells. 相似文献
2.
T Endo M Ikeda M Ohmori E Anzai K Haraguchi T Onaya 《Biochemical and biophysical research communications》1992,187(2):887-893
We have produced rabbit antibody against a synthetic peptide corresponding to N-terminal region of the extracellular domain of human thyrotropin receptor (hTSH-R) (N peptide, aminoacid residues 29-57). Western blot analysis revealed that N-peptide antibody recognized recombinant hTSH-R stably expressing in CHO-K1 cells as a mol. wt. about 104 kDa regardless in the presence or absence of disulfide-reducing agent. The band was not detected in untransfected CHO-K1 cells and no band was also stained by the antibody absorbed with N-peptide. In a reducing condition, the antibody also bound the rat receptor from FRTL5 cells as the same molecular size (104 kDa). These results clearly indicate that TSH-R is composed of a single subunit and that two subunit model for the TSH-R may reflect artifactual proteolytic cleavage of the receptor during membrane preparation. 相似文献
3.
S. G. Nugroho J. Lumbanraja H. Suprapto Sunyoto H. Haraguchi M. Kimura 《Plant and Soil》1996,181(2):287-293
Yearly and seasonal (rainy and dry seasons) variations of CH4 emission from a Sumatra paddy field were measured for 3 years. The mean CH4 emission rates during the growth period were in the range of 16.0–26.1 mg CH4 m-2 h-1 for the chemical fertilizer plots and 23.3–34.9 mg CH4 m-2 h-1 for the plots with rice straw application, respectively. The increase in the amounts of CH4 emission by rice straw application were from 1.3 to 1.6 times. There was no significant difference in the mean CH4 emission rates between rainy and dry seasons.Total amounts of CH4 emitted during the period of rice growth were in the ranges of 29.5–48.2 and 43.0–64.6 g CH4 m-2 for the plots applied with chemical fertilizer and those with rice straw application, respectively. Nearly the same amounts of CH4 were emitted in the first and second half of the growth period, irrespective of rice straw application. 相似文献
4.
A case of transient hypothyroidism in the course of hypokalemic myopathy is reported. A 69-year-old woman had severe muscle weakness and marked potassium deficiency associated with alkalosis during treatment with thiazide diuretics. The cause of muscle weakness proved to be hypokalemic myopathy confirmed by clinical findings and muscle biopsy. After the episode of hypokalemic myopathy, serum levels of thyroid hormone were lowered (T4; 3.8 micrograms/dl, T3; 54 ng/dl) and that of TSH was elevated (25.1 microU/ml). Antithyroid microsomal antibody was positive (1:25600) and anti-thyroglobulin antibody was negative. About one month after potassium supplement, her thyroid functions returned to normal, along with normalization of serum potassium level. This is the first documented case report of hypokalemic myopathy accompanied by transient hypothyroidism in a patient with autoimmune thyroiditis. We suggest that this transient hypothyroidism might be induced by hypokalemia during the course of autoimmune thyroiditis. 相似文献
5.
6.
nanos1: a mouse nanos gene expressed in the central nervous system is dispensable for normal development 总被引:2,自引:0,他引:2
Haraguchi S Tsuda M Kitajima S Sasaoka Y Nomura-Kitabayashid A Kurokawa K Saga Y 《Mechanisms of development》2003,120(6):721-731
A mouse nanos (nanos1) gene was cloned and its function was examined by generating a gene-knockout mouse. The nanos1 gene encodes an RNA-binding protein, which contains a putative zinc-finger motif that exhibits similarity with other nanos-class genes in vertebrates and invertebrates. Although nanos1 is not detected in primordial germ cells, it is observed in seminiferous tubules of mature testis. Interestingly, maternally expressed nanos1 is observed in substantial amounts in oocytes, but the amount of maternal RNA is rapidly reduced after fertilization, and the transient zygotic nanos1 expression is observed in eight-cell embryos. At 12.5 days postcoitum, nanos1 is re-expressed in the central nervous system and the expression continues in the adult brain, in which the hippocampal formation is the predominant region. The nanos1 -deficient mice develop to term without any detectable abnormality and they are fertile. No significant neural defect is observed in terms of their behavior to date. 相似文献
7.
Kazuhiro Haraguchi Shigeru Saito Hiromichi Tanaka Tadashi Miyasaka 《Nucleosides, nucleotides & nucleic acids》2013,32(2-4):483-493
Abstract Pummerer-type rearrangement of uracil nucleosides having a phenylseleno group in the 2′-, 3′-, and 5′-positions took place upon oxidation with MCPBA in CH2Cl2 followed by treatment with acid anhydrides to yield α-acyloxyselenides. 相似文献
8.
The sweetpotato weevil Cylas formicarius (Fabricius) (Coleoptera: Brentidae) and West Indian sweetpotato weevil Euscepes postfasciatus (Fairmaire) (Coleoptera: Curculionidae) are major pests of sweet potato Ipomoea batatas (L.) Lam., in tropical and subtropical regions. Effects of intra‐ and inter‐specific competition on the number of progeny (fecundity), body weight and developmental time of C. formicarius and E. postfasciatus were examined in single‐ and mixed‐species treatments under laboratory conditions. Cylas formicarius tended to outcompete E. postfasciatus, whereas E. postfasciatus rather than C. formicarius tolerated higher conspecific densities. We discuss the implications of the results for pest management and resource partitioning of pestiferous weevils. 相似文献
9.
Akari Nishigaki Mihoko Maruyama Munenori Numata Chisako Kanzaki Shun‐Ichi Tanaka Hiroshi Y. Yoshikawa Masayuki Imanishi Masashi Yoshimura Yusuke Mori Kazufumi Takano 《Engineering in Life Science》2020,20(9-10):395-401
It is known that interfaces have various impacts on crystallization from a solution. Here, we describe crystallization of acetaminophen using a microflow channel, in which two liquids meet and form a liquid–liquid interface due to laminar flow, resulting in uniform mixing of solvents on the molecular scale. In the anti‐solvent method, the microflow mixing promoted the crystallization more than bulk mixing. Furthermore, increased flow rate encouraged crystal formation, and a metastable form appeared under a certain flow condition. This means that interface management by the microchannel could be a beneficial tool for crystallization and polymorph control. 相似文献
10.