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排序方式: 共有1339条查询结果,搜索用时 15 毫秒
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Y Furuya Y Yoshida T Katayama S Yamamoto A Kawamura 《Canadian Metallurgical Quarterly》1993,31(6):1637-1640
Polymerase chain reaction (PCR) with nested primer pairs was used to diagnose scrub typhus and identify the Rickettsia tsutsugamushi serotype. The primer pairs used for PCR were designed on the basis of the nucleotide sequence of the gene that encodes the 56-kDa antigen. Serotype-specific primers were used in the second PCR amplification. Five serovariants, the Gilliam, Karp, Kato, Kawasaki, and Kuroki strains of R. tsutsugamushi, were identified by nested PCR. In addition, the serotype identified by PCR with DNA from blood clots was the same as that of the strain isolated from five patients with scrub typhus. These findings indicate that this method is useful for diagnosis and identification of the rickettsial serotype in infected patients. 相似文献
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J.H. Kaneko T. Tanaka S. Kawamura Y. Oshiki K. Tsuji M. Katagiri K. Ochiai T. Nishitani F. Fujita A. Homma T. Sawamura T. Iida M. Furusaka 《Diamond and Related Materials》2005,14(11-12):2027
Radiation detector was made of a high-quality CVD polycrystalline diamond composed of frost column like structure diamond grains, and induced charge distribution spectra and drift velocities were measured by using alpha particles. As a result, the CVD polycrystalline achieved maximum induced charge of 83% of HP/HT type IIa diamond. Moreover, the CVD crystal had lower charge loss on electrons compared with the HP/HT type IIa diamond. Drift velocities of electrons and holes were ve = 7.7 × 104 and vh = 7.3 × 104cm/s at an electric field of 20 kV/cm, respectively. In addition, response function measurement for 14 MeV neutrons was carried out. 相似文献
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The authors reports the fabrication of a flip-chip InGaAsP-InAlAs superlattice avalanche photodiode using gas source molecular beam epitaxy. The incident light reaches the InGaAs photoabsorption layer through the InP substrate and an InGaAsP-InAlAs superlattice multiplication region which are transparent for wavelengths of 1.55 and 1.3 μm. The light reflection by the electrode enables the absorption layer to be as thin as 0.8 μm without significantly reducing the quantum efficiency. A maximum bandwidth of 17 GHz was obtained at a low multiplication factor because the transit time through the absorption layer is reduced 相似文献
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Hiroyuki?Y.?SuzukiEmail author Hidenori?Kuroki 《Metals and Materials International》2004,10(2):185-191
A new compacting method for powder materials is presented. High-Speed Centrifugal Compaction Process (HCP) utilizes centrifugal
force of about 10,000 g for the compaction. HCP is suitable for net shape compaction of fine sub-micron and nano-powders.
HCP possesses a unique compacting mechanism that is different from other colloidal processes including Pressure Casting (PC),
and has a number of useful characteristics. HCP has a higher compacting speed than PC, wide applicability for net shape formation,
as well as a defect removing function. Because of homogeneous and flawless green microstructure, HCP alumina shows superior
sinterability and higher strength and hardness than most of other aluminas.
This article is based on a presentation made in the symposium “The 3rd KIM-JIM Joint Symposium on Advanced Powder Materials“,
held at Korea University, Seoul, Korea, October 26–27, 2001 under auspices of The Korean Institute of Metals and Materials
and The Japan Institute of Metals. 相似文献
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Imoto Taiji; Ueda Tadashi; Tamura Tomohiro; Isakari Yoshimasa; Abe Yoshito; Inoue Makoto; Miki Takeyoshi; Kawano Keiichi; Yamada Hidenori 《Protein engineering, design & selection : PEDS》1994,7(6):743-748
Mutations around His15 which lie far away from the active site,stimulated glycol chitin activity of lysozyme at physiologicaltemperature. Del-Argl4Hisl5 lysozyme, a mutant lysozyme whoseArgl4 and Hisl5 were deleted together, and has the highest activityamong these mutant lysozymes, had a similar binding abilityto a trimer of N-acetyl-glucosamine, a substrate analogue, relativeto native lysozyme. This suggests that the increased activitywas due to an increased kcat in the catalysis reaction. TheH-D exchange rate of the N-1 proton in the Trp63 which is locatedin the active site cleft, was enhanced in the Del-Argl4Hisl5lysozyme, while 2-D proton NMR analysis revealed no conformationalchange around Trp63. We conclude that some sort of fluctuationat the active site might be required for the manifestation ofactivity. This theory is supported by the finding that the Del-Argl4Hisl5lysozyme showed a shift in temperature dependency of activityto lower temperatures compared with that of native lysozyme. 相似文献
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Maeda Yoshitake; Koga Hiroshi; Yamada Hidenori; Ueda Tadashi; Imoto Taiji 《Protein engineering, design & selection : PEDS》1995,8(2):201-205
To increase the folding yield of concentrated reduced lysozyme,we developed a renaturation method by means of dialysis fromconcentrated urea with redox agents. After lysozyme was incubatedin the reducing buffer (8 M urea solution) with oxidized glutathione,renaturation of reduced lysozyme was started by dialysis againstthe dialyzing buffer containing 8 M urea with redox agents.The urea concentration of the dialyzing bottle was graduallydiluted with dialyzing buffer without urea at a flow rate of0.1 ml/min by high pressure pump. Using this systematic dialysis,a concentration as high as 5 mg/ml of reduced lysozyme couldbe renaturated in 80% yield, while the folding yield was <5%even at a concentration of 1 mg/ml using a conventional rapiddilution method [Goldberg et al. (1991) Biochemistry, 30, 27902797].Therefore, it was concluded that gentle removal of urea fromdenatured proteins, dissolved in concentrated urea solution,by means of dialysis should be useful to renature denaturedproteins effectively. 相似文献