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PURPOSE: We investigated Sertoli cells in testicular biopsies with carcinoma in situ (CIS) in respect to cytokeratin expression to elucidate the status of Sertoli cell differentiation adjacent to CIS in human testes. Cytokeratin 18 intermediate filaments indicate a state of undifferentiation usually observed in Sertoli cells of prepubertal testes. MATERIALS AND METHODS: 29 testicular biopsies presenting CIS were investigated by means of immunohistochemistry, using a polyclonal antibody against placental-alkaline phosphatase to detect CIS cells and a monoclonal antibody against human cytokeratin 18 to show expression of cytokeratin 18 intermediate filaments in Sertoli cells. RESULTS: All tubules bearing CIS showed positive cytokeratin expression of Sertoli cells if tubules were devoid of normal germ cells. However, a total of 13 specimen revealed CIS cells together with normal germ cells. In the presence of CIS cells together with round or elongated spermatids, adjacent Sertoli cells did not express cytokeratin immunoreactivity. In the case of combined presence of CIS and spermatogonia and primary spermatocytes, Sertoli cells could be found either immunopositive or immunonegative, and were positive in tubules with CIS and spermatogonia only. CONCLUSIONS: Sertoli cells associated with CIS cells undergo a process of dedifferentiation, seen by the re-expression of cytokeratin intermediate filaments. We suggest that this dedifferentiation results in a loss of Sertoli cell function and leads to a cessation of spermatogenic activity. 相似文献
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PE Borchardt SM Quadri RS Freedman HM Vriesendorp 《Canadian Metallurgical Quarterly》1997,44(3):283-293
Sensory-perceptual abnormalities in people with autism are discussed from two perspectives: published firsthand accounts and existing psychological research evidence. A range of abnormalities, including hyper- and hyposensitivity, sensory distortion and overload, and multichannel receptivity and processing difficulties, are described in firsthand accounts and frequently portrayed as central to the autistic experience. A number of dangers are inherent in uncritically accepting these accounts at face value and in any wider generalization to the autistic population as a whole. Evidence from clinical studies suggests that unusual sensory responses are present in a majority of autistic children, that they are manifested very early in development, and that they may be linked with other aspects of autistic behavior. In addition, experimental studies using a range of indices have found evidence of unusual responses to sensory stimuli in autistic subjects. However the clinical and experimental research to date suffers from serious methodological limitations and more systematic investigation is warranted. Key issues for future psychological research in the area are identified. 相似文献
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介绍了马兰矿南五采区煤仓施工方法,认为利用反井钻机施工煤仓,工艺简单、安全高效,同时就煤仓施工中存在的问题提出了建议. 相似文献
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随着计算机网络的迅速发展,网络安全问题正变得日益重要。文章介绍了计算机网络系统IP层安全概况和入侵者对IP层常采用的入侵手段,以及为防止这种入侵所采取的措施。 相似文献
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略论中国传统合院住宅形式的固化与沿用 总被引:1,自引:1,他引:0
合院式住宅是中国人沿用了二千多年的典型形制,除了其能满足采光、通风的要求和建造相对简化外,中国传统社会早期的中霤祭祀和以五行为基本元素的统括万物的宇宙模式对于中国式合院住宅的固化与沿用,也起了不可忽视的作用。 相似文献
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神马尼龙66高强力轻型帘布在保持帘布高强力、尺寸稳定性、耐热性、耐疲劳、耐冲击的良好特性的前提下,采用SNT高强度工业丝生产技术,充分挖掘工业丝的强力优势,把富余的强力通过进一步降低纤维密度转化为帘布面积优势的扩大,从而提高轮胎生产的帘布出胎率,使轮胎生产厂享受尼龙66帘布品质的同时,实现降低成本的目的。 相似文献
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Jiayong Cao Yu Zhu Jinsong Wang Wensheng Yin Guanghong Duan 《IEEE transactions on magnetics》2004,40(6):3490-3494
This paper investigates three kinds of permanent-magnet arrays used in planar motors with polarity centers distributed in the lattices of a matrix. The magnetic field of every kind of magnet array is analyzed by analytical methods. First, we give a Laplace equation of magnetic scalar potential and a series of boundary conditions. Then, we derive the analytical expressions of magnet field strength and magnet flux density in the air gap of each kind of magnet array by the method of separation of variables. Finally, we compare the three types of magnet arrays on the basis of the analytical solutions. 相似文献