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1.
Four murine monoclonal antibodies (mAbs) designated as C9E8, A10, G12, and G8 which recognized both Serpulina hyodysenteriae and S. innocens were produced and characterized. The mAbs reacted with whole cell antigens in ELISA, indirect immunofluorescence and immunoblot assays. The mAbs did not show any cross reactivity in rapid dot ELISA or immunoblot assay with Leptospira icterohemorrhagiae, Campylobacter jejuni and Escherichia coli. Treatment of whole cell suspension with proteinase K and sodium periodate indicated that the reacting epitopes of the mAbs were protein in nature. The genus-specific antigens were identified as heat-stable proteins with molecular weight in the range of 26 to 45 kDa. Immunofluorescence and immunogold labelling studies showed that the antibody-binding epitopes were exposed on the outer-surface of the spirochaetal cell wall. The mAbs inhibited growth of reference strains of both S. hyodysenteriae and S. innocens in vitro but failed to cause agglutination. The detection of spirochaetal forms directly in fecal smears or paraffin-embeded tissue sections from experimentally infected pigs indicated that such mAbs were potentially useful for the diagnosis of swine spirochaetosis. This is the first report of mAbs identifying and characterizing common antigens of porcine Serpulina. 相似文献
2.
Efficient compression and network adaptive video coding for distributed video surveillance 总被引:1,自引:1,他引:0
The availability of cheap network based video cameras and the prevalence of wireless networks has lead to a major thrust towards
deployment of large scale Distributed Video Surveillance (DVS) systems. This has opened up an important area of research to
deal with the issues involved in DVS system for efficient collection and transmission of large scale video streams from the
cameras at the guarded sites, to the end users in possibly constrained network conditions. In this paper, we propose a framework
based on content-based video classification and scalable compression scheme to provide a robust bandwidth efficient video
transmission for DVS. The scheme builds on a Discrete Wavelet Transform (DWT) based Color-Set Partitioning for Hierarchical
Trees (CSPIHT) coding to obtain a scalable bitstream. Wavelet domain segmentation and compression assists in development of
a DVS architecture. The architecture includes a novel module for dynamic allocation of Network bandwidth based on the current
available resources and constraints. Different frame constituents are optimally coded based on their relative significance,
perceptual quality, and available estimate of network bandwidth. Experimental result over different video sequences and simulations
for Network conditions demonstrate the efficient performance of the approach. 相似文献
3.
This paper presents OS-Guard(On-Site Guard), a novel on-site signature based framework for multimedia surveillance data management. One of the major concerns in widespread
deployment of multimedia surveillance systems is the enormous amount of data collected from multiple media streams that need
to be communicated, observed and stored for crime alerts and forensic analysis. This necessitates investigating efficient
data management techniques to solve this problem. This work aims to tackle this problem, motivated by the following observation,
more data does not mean more information. OS-Guard is a novel framework that attempts to collect informative data and filter out non-informative data on-site, thus
taking a step towards solving the data management problem. In the framework, both audio and video cues are utilized by extracting
features from the incoming data stream and the resultant real valued feature data is binarized for efficient storage and processing.
A feature selection process based on association rule mining selects discriminant features. A short representative sample
of the whole database is generated using a novel reservoir sampling algorithm that is stored onsite and used with an support
vector machine to classify an important event. Initial experiments for a Bank ATM monitoring scenario demonstrates promising
results. 相似文献
4.
Céline Cohen Rory Giles Victoria Sergeyeva Nitesh Mittal Patrick Tabeling Djamal Zerrouki Jean Baudry Jérôme Bibette Nicolas Bremond 《Microfluidics and nanofluidics》2014,17(5):959-966
The capacity of microfluidic technology to fabricate monodisperse emulsion droplets is well established. Parallelisation of droplet production is a prerequisite for using such an approach for making high-quality materials for either fundamental or industrial applications where product quantity matters. Here, we investigate the emulsification efficiency of parallelised drop generators based on a flow-focusing geometry when incorporating the role of partial wetting in order to make emulsion droplets with a diameter below 10 μm. Confinement intrinsically encountered in microsystems intensifies the role played by interfaces between liquids and solids. We thus take advantage of partial wetting to enhance the maximum confinement accessible due to liquid flow focusing. We compare the performances brought by partial wetting to more established routes such as step emulsification. We show that the step configuration and the partial wetting regime are both well suited for being parallelised and thus open the way to the production of fine and calibrated emulsions for further applications. Finally, this new route of emulsification that exploits partial wetting between the fluids and the channel walls opens possibilities to the formation of substantially smaller droplets, as required in many fields of application. 相似文献
5.
The cooking kinetics of meat emulsions containing various fillers was determined by monitoring changes in hydration properties such as cooking loss and water-holding capacity during smokehouse cooking. Press juice, consumer cook test and emulsion stability of cooked product were also determined. The fillers used were buttermilk powder, corn starch, microcrystalline cellulose, modified corn starch, modified wheat flour, soy-protein concentrate and whey-protein concentrate. The cooking process was modelled using reaction kinetics and Eyring's absolute reaction rate theory. Enthalpy and entropy changes of activation were calculated for various properties and fillers. 相似文献
6.
R.C. Mittal 《国际计算机数学杂志》2015,92(10):2139-2159
A technique to approximate the solutions of nonlinear Klein–Gordon equation and Klein–Gordon-Schrödinger equations is presented separately. The approach is based on collocation of cubic B-spline functions. The above-mentioned equations are decomposed into a system of partial differential equations, which are further converted to an amenable system of ODEs. The obtained system has been solved by SSP-RK54 scheme. Numerical solutions are presented for five examples, to show the accuracy and usefulness of proposed approach. The approximate solutions of both the equations are computed without using any transformation and linearization. The technique can be applied with ease to solve linear and nonlinear PDEs and also reduces the computational work. 相似文献
7.
Abstract: Pedestrian detection techniques are important and challenging especially for complex real world scenes. They can be used for ensuring pedestrian safety, ADASs (advance driver assistance systems) and safety surveillance systems. In this paper, we propose a novel approach for multi-person tracking-by-detection using deformable part models in Kalman filtering framework. The Kalman filter is used to keep track of each person and a unique label is assigned to each tracked individual. Based on this approach, people can enter and leave the scene randomly. We test and demonstrate our results on Caltech Pedestrian benchmark, which is two orders of magnitude larger than any other existing datasets and consists of pedestrians varying widely in appearance, pose and scale. Complex situations such as people occluded by each other are handled gracefully and individual persons can be tracked correctly after a group of people split. Experiments confirm the real-time performance and robustness of our system, working in complex scenes. Our tracking model gives a tracking accuracy of 72.8% and a tracking precision of 82.3%. We can further reduce false positives by 2.8%, using Kalman filtering. 相似文献
8.
9.
Kaur Amanpreet Gupta Govind P. Mittal Sangeeta 《Wireless Personal Communications》2022,123(2):1625-1667
Wireless Personal Communications - Node localization is one of the essential services where sensor nodes in the wireless sensor network collaborate to provide location information of sensor nodes... 相似文献
10.
For precise industrial applications, a manipulator must have high positioning accuracy and repeatability. However, variations in performance of the manipulator are attributed to improper selection of dimensions of design and process parameters and its tolerance. There have been almost no attempts to optimize these parameters and tolerance of the manipulator, by which performance variations will be minimum. This paper presents an offline approach to select optimal parameters and tolerance simultaneously which minimizes the manufacturing cost and delivers the target performance level. To determine the optimal parameters and tolerances, a modification in differential evolution algorithm is proposed, which helps in incorporating the effect of uncertainty in optimization process. Proposed approach has been illustrated by selecting optimal parameters and tolerance of a 2-DOF RR planar manipulator. The performance of this approach has been compared with modified genetic algorithm. It is observed that differential evolution algorithm provides optimal results with lesser computations compared to genetic algorithm. 相似文献