Although lipases are widely applied in a wide variety of reactions, available information on the factors that are responsible for the substrate specificities of lipases from different sources is scarce. In this paper, nine lipase‐producing microorganism strains were isolated from oil‐containing samples. The properties of these enzymes, including pH optima, temperature optima, thermal stability, and pH stability, vary significantly with the different sources from which these lipases were isolated. The substrate specificities of the nine lipases, including fatty acid and positional specificities, were also studied. The fatty acid specificities of the nine lipases were observably different toward 15 substrates with different carbon chain lengths, different saturation degrees and different side chains. The lipases from S3Penicillium citrinum (PCL), MJ1Aspergillus niger (ANL), MJ2Aspergillus oryzae (AOL), YM Bacillus coughing (BCL), S9Geotrichum candidum (GCL), and S11Candida lypolytica (CLL) showed the strongest specificities to short‐chain esters, and the other lipases showed strong selectivity for medium‐ or long‐chain and branched esters. The positional specificities were examined by analyzing the hydrolytic products of triolein catalyzed by the nine lipases, using TLC. The lipases can be mainly classified into two groups by their specificities for the ester bond at position 2 of triglycerides; one group can selectively hydrolyze the ester bond at position 2 of the triglycerides, the other group cannot. All these nine lipases were divided into four groups by hierarchical clustering analysis on the basis of the results of fatty acid and positional specificities. 相似文献
We propose a novel online multiple object tracker taking structure information into account. State-of-the-art multi-object tracking (MOT) approaches commonly focus on discriminative appearance features, while neglect in different levels structure information and the core of data association. Addressing this, we design a new tracker fully exploiting structure information and encoding such information into the cost function of the graph matching model. Firstly, a new measurement is proposed to compare the structure similarity of two graphs whose nodes are equal. With this measurement, we define a complete matching which performs association in high efficiency. Secondly, for incomplete matching scenarios, a structure keeper net (SKnet) is designed to adaptively establish the graph for matching. Finally, we conduct extensive experiments on benchmarks including MOT2015 and MOT17. The results demonstrate the competitiveness and practicability of our tracker.