Isolation and characterization of endometrial leukocyte populations by flow cytometry

Potentialities of the flow cytornetry method in studies of the qualitative and quantitative composition of endometrial immunocompetent cells are demonstrated. The studied leukocyte populations are found to occur in negligible quantities in the endometrium. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 117, N ^o 1, pp. 68–71, January, 1994.     

Ratio between the number of Th1 and Th2 lymphocytes in the peripheral blood and concentration of proinflammatory cytokines in lochia of women with postpartum endometritis

We estimated the percentage of CD4⁺ lymphocytes synthesizing IFN-γ and IL-4 in the peripheral blood and measured the concentrations of IL-1, IL-6, and TNF-α in lochia of patients with postpartum endometritis. T lymphocytes with intracellular IFN-γ prevailed in women with physiological course of the postpartum period. The number of cells containing IL-4 increased in patients with postpartum endometritis. On days 5–7 after childbirth the concentrations of proinflammatory cytokines IL-1β and TNF-α in lochia of these patients were higher than in healthy postpartum women (by 1.4 and 2.7 times, respectively). The concentrations of cytokines IL-1β, TNF-α, and IL-6 increased most significantly in patients with postpartum endometritis and active viral infection (by 3.4, 4.2, and 1.6 times, respectively). __________ Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 140, No. 12, pp. 622–624, December, 2005     

Effect of embryonic bone tissue on bone regeneration

Fragmented embryonic bone tissue stimulates bone regeneration. Bone formation starts not from implanted embryonic fragments, but in intact periosteum and endosteum containing cambial cells of the osteodifferon. In rabbits, recovery of damaged radial bone after implantation of fragmented embryonic bone tissue into bone defect was associated with a pronounced periosteal reaction and focal resorption of intact ulnar bone. Consolidation of damaged radial bone without implantation of fragmented embryonic bone tissue was incomplete in all experimental animals. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 130 No. 10, pp. 469–474, October, 2000     

In Vitro Development of Neural Progenitor Cells from Human Embryos

Behavior of stem/progenitor cells from the brain of human embryos during in vitro culturing was studied. Cultured cells from human embryonic brain developed and formed neurospheres heterogeneous by their cell composition. In a serum-containing medium some cells underwent differentiation by the neuronal pathway, while others remained in the stem state.     

Correlation of prolactin concentration with levels of other hormones and with ultrastructural morphometric parameters of the testes in the human fetus

Consideration is given to the correlations between variations of the prolactin concentration and other hormonal parameters in the human embryonic period. The interrelation between the prolactin concentration and ultrastructural parameters of the testes is also traced. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 119, N ^o 6, pp. 654–656, June, 1995 Presented by O. V. Volkova, Member of the Russian Academy of Medical Sciences     

Further study on the vascular basis for the reimplantation of the hand amputated through the palm

Summary Based on the anatomic data obtained from earlier studies on the vascular anatomy of the hand, the vascular architecture in the palm of the hand was studied on 60 sides of unembalmed adult upper extremities. Each palm was divided into 64 squares by 8 sagittal and 8 transverse sections. The vascular architecture in these squares and the arterial relations between them were observed and measured by angiography, operative microscopic dissection and computerised three-dimensional reconstruction. According to the pattern of the blood-vessels, the amputated palms can be classified into 4 types. The anatomic basis for the vascular anastomosis in each type is defined. There are three key-areas for the blood-supply of the palm and their significance is discussed. Apart from the 4 types of transversely amputated palms, the repair programe of the blood-vessles in 4 types of common obliquely amputated palms are also discussed.

---

Etude complémentaire de l'anatomie vasculaire de la main pour la réimplantation des amputations transpalmaires

Résumé Sur la base de données anatomiques obtenues lors de précédents travaux sur l'anatomie vasculaire de la main, l'architecture vasculaire palmaire a été étudiée sur 60 extrémités supérieures de cadavres d'adultes, non embaumés. Chaque paume a été divisée en 64 carrés par 8 sections sagittales et 8 sections transversales. L'architecture vasculaire à l'intérieur des carrés et les relations artérielles entre eux ont été étudiées et mesurées par angiographie, dissection au microscope opérateur et reconstruction computérisée en 3D. Les paumes amputées ont été regroupées en 4 types d'après la distribution des vaisseaux sanguins. Les données anatomiques concernant les anastomoses vasculaires sont précisées. Il existe trois zones clés pour l'irrigation de la paume. Leur importance quant à l'irrigation de la main est exposée. Outre la division des paumes amputées transversalement en 4 types, le programme de réparation de vaisseaux dans les 4 types d'amputations obliques communes de la paume et aussi discuté.

     

Comparative study of the effects of trophoblastin and polydeoxyribonucleotide preparations on cultured skin fibroblast

We studied the effects of trophoblastin and polydeoxyribonucleotide on the morphology and functional characteristics of fibroblasts in the culture of skin cells. Positive effects of trophoblastin and polydeoxyribonucleotide on fibroblast growth and survival during culturing were demonstrated. Trophoblastin exhibited similar effects on cells of early and late passages. Polydeoxyribonucleotide exhibited a more pronounced effect on cells of late passages, whose growth activity decreased with age. Being a multicomponent preparation, trophoblastin stimulated processes leading to positive dynamic of cell development and vital activity. Polydeoxyribonucleotide had a favorable impact on functioning of basal cell structures at the level of cell nucleus and maintained high level of cells with complete DNA set. __________ Translated from Kletochnye Tehnologii v Biologii i Medicine, No. 3, pp. 173–177, July, 2007     

Comparative analysis of differentiation and behavior of human neural and mesenchymal stem cells In Vitro and In Vivo

Comparative analysis of differentiation of human neural and mesenchymal stem cells in tissue culture and after transplantation into the brain was carried out using the same antibody set. Neural stem cells differentiated into all types of neural cells, are retained after transplantation, migrate, and form reciprocal relationships with the recipient brain. Mesenchymal stem cells were incapable of neural development under conditions of common culturing or after transplantation and retained the fibroblast-like status. Recipient filaments grew into mesenchymal stem cell transplants containing no neural cells due to local changes in the extracellular matrix at the site of transplantation. __________ Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 1, pp. 44–52, January, 2006     

Immunomorphological characteristics of endometrium in women with chronic endometritis

The number of monocytes, macrophages (CD14⁺), and large granular lymphocytes (CD56⁺) in the endometrium increased in chronic endometritis. These changes are an unfavorable prognositic signs for the onset of pregnancy. Combination therapy improved the phenotypic composition of endometrial cells. __________ Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 141, No. 1, pp. 113–115, January, 2006